FlyBase Allele Report: Dmel\Mrp4[EP3221]

General Information

Symbol

Dmel\Mrp4^EP3221

Species

D. melanogaster

Name

FlyBase ID

FBal0123254

Feature type

allele

Associated gene

Dmel\Mrp4

Associated Insertion(s)

P{EP}Mrp4^EP3221

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-, Kraut et al., 2001)

Progenitor genotype

Associated Insertion(s)

P{EP}Mrp4^EP3221

Cytology

Description

P{EP}Mrp4^EP3221 is inserted 88 bp upstream of the Mrp4 transcription start site.

(Huang and Haddad, 2007)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:11,569,162..11,569,162 [+]

(Huang and Haddad, 2007, Gene Disruption Project members, 2001-, Kraut et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

anterior fascicle & synapse | supernumerary, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

larval intersegmental nerve, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

Detailed Description

Statement

Reference

Flies expressing Mrp4^EP3177 using Scer\GAL4^da.G32 or Scer\GAL4^Act.PU or Scer\GAL4^elav.PU display a profound increase in recovery time after anoxia compared with controls.

Mrp4^EP3177/Mrp4^EP3221 flies show an increase in recovery time after anoxia compared with controls.

No pupae form when Mrp4^EP3221 is expressed with Scer\GAL4^da.G32 under chronic hypoxia conditions. This is in contrast to controls which show no larval developmental defects and die during late pupal stages under the same conditions.

(Huang and Haddad, 2007)

Mutant larvae expressing EP3221^EP3221 under the control of Scer\GAL4^elav-C155 have ISN neuron pathfinding defects. Mutant larvae expressing EP3221^EP3221 under the control of Scer\GAL4^elav-C155 have excessive or ectopic synapses.

(Kraut et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

EP3221^EP3221

Mrp4^EP3221

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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