FlyBase Allele Report: Dmel\UQCR-14L[GD9481]

General Information

Symbol

Dmel\UQCR-14L^GD9481

Species

D. melanogaster

Name

FlyBase ID

FBal0201980

Feature type

allele

Associated gene

Dmel\UQCR-14L

Associated Insertion(s)

Carried in Construct

P{GD9481}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD9481}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD9481

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal stress response | RU486 conditional, with Scer\GAL4^{αTub84B.Switch.PK}

(Copeland et al., 2009)

female semi-fertile | RU486 conditional, with Scer\GAL4^{αTub84B.Switch.PK}

(Copeland et al., 2009)

lethal, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

lethal - all die before end of larval stage, with Scer\GAL4^da.G32

(Copeland et al., 2009)

lethal - all die before end of larval stage | RU486 conditional, with Scer\GAL4^{αTub84B.Switch.PK}

(Copeland et al., 2009)

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

long lived | female | RU486 conditional, with Scer\GAL4^{αTub84B.Switch.PK}

(Copeland et al., 2009)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during larval stage, with Scer\GAL4^da.G32

(Copeland et al., 2009)

some die during larval stage | RU486 conditional, with Scer\GAL4^{αTub84B.Switch.PK}

(Copeland et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

adult heart | RU486 conditional, with Scer\GAL4^{Hand.ΔVM.Switch}

(Tricoire et al., 2014)

larval brain | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression of CG17856^GD9481 under the control of Scer\GAL4^{Hand.ΔVM.Switch} (initiated by feeding flies RU486 throughout development and adulthood) results in significant heart dilatation. This phenotype is partially rescued upon treatment with methylene blue.

(Tricoire et al., 2014)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in an increased number of GFP-positive aggregates within neuroblast lineage cells in the larval brain compared to controls (these aggregates may indicate cell death or a disturbance of internal cellular structures).

(Neumüller et al., 2011)

Adults expressing UQCR-14L^GD9481 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

(Schnorrer et al., 2010)

Ubiquitous expression using Scer\GAL4^Switch.tub, induced during larval development and adulthood, prolongs life span in female flies and has variable effects on male life span. Female fertility is reduced. Flies also show improved tolerance to paraquat poisoning, but do not show improved starvation resistance. High level induction causes larval lethality.

Ubiquitous expression using Scer\GAL4^Switch.tub from the onset of adulthood only does not confer robust longevity effects in male or female flies. However, female fertility is reduced.

(Copeland et al., 2009)

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 0% or 40-50% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 40-50% or 50-60% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 20-30% or 50-60% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

9481

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

UQCR-14L^GD9481, Scer\GAL4^insc-Mz1407 is a non-suppressor of abnormal neuroanatomy phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407

(Lee et al., 2013)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

UQCR-14L^GD9481, Scer\GAL4^insc-Mz1407 is a non-suppressor of type II neuroblast | increased number phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407

(Lee et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of CG17856^GD9481 does not suppress the increase in the number of type II neuroblasts which is seen in the central brain of animals expressing N^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of Scer\GAL4^insc-Mz1407.

(Lee et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v33016