FlyBase Allele Report: Dmel\DopEcR[c02142]

General Information

Symbol

Dmel\DopEcR^c02142

Species

D. melanogaster

Name

FlyBase ID

FBal0161995

Feature type

allele

Associated gene

Dmel\DopEcR

Associated Insertion(s)

PBac{PB}DopEcR^c02142

Carried in Construct

Also Known As

DopEcR^PB1

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

Mutagen

piggyBac activity

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Ishimoto et al., 2013)

Mutagen

piggyBac activity

(Bloomington Drosophila Stock Center, 2004.11.6)

Progenitor genotype

Associated Insertion(s)

PBac{PB}DopEcR^c02142

Cytology

Description

Insertion in the second intron.

(Ishimoto et al., 2013)

Allele components

Component

Use(s)

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:4,378,274..4,378,274 [+]

(Kajtor et al., 2025, Saito et al., 2025, Aranda et al., 2017, Ishimoto et al., 2013, Inagaki et al., 2012, Bloomington Drosophila Stock Center, 2004.11.6)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Petruccelli et al., 2016)

CEA with DopEcR^GAL4

(Petruccelli et al., 2016)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of alcohol use disorder

is exacerbated by Egfr^KK100051

(Petruccelli et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior | chemical conditional

(Aranda et al., 2017)

abnormal courtship behavior | male limited, with DopEcR^RNAi.UAS.cEa, Scer\GAL4^unspecified

(Evans et al., 2014)

abnormal learning, with DopEcR^RNAi.UAS.cEa, Scer\GAL4^unspecified

(Evans et al., 2014)

abnormal memory | adult stage

(Ishimoto et al., 2013)

abnormal memory | adult stage (with Df(3L)ED4341)

(Ishimoto et al., 2013)

abnormal neurophysiology | adult stage

(Ishimoto et al., 2013)

abnormal neurophysiology | adult stage (with Df(3L)ED4341)

(Ishimoto et al., 2013)

abnormal tarsal response | chemical conditional

(Inagaki et al., 2012)

abnormal tarsal response | nutrition conditional

(Inagaki et al., 2012)

chemical resistant | adult stage

(Aranda et al., 2017, Petruccelli et al., 2016)

chemical resistant | adult stage (with DopEcR^GAL4)

(Petruccelli et al., 2016)

chemical sensitive | adult stage

(Petruccelli et al., 2016)

chemical sensitive | adult stage (with DopEcR^GAL4)

(Petruccelli et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

DopEcR^c02142 homozygous adults show an increased resistance to ethanol as compared to controls: they show an increased time until sedation, despite an apparently normal tolerance development following daily exposure; they also show suppression of a normal ethanol-induced disinhibition, as shown by the virtual lack of inter-male courtship upon continual exposure.

(Aranda et al., 2017)

DopEcR^c02142 homozygous and (to a lesser extent) heterozygous as well as DopEcR^c02142/DopEcR^GAL4 transheterozygous adults show resistance to ethanol-induced sedation: it takes significantly longer for them cease all movement upon ethanol exposure, although they begin to lose posture around the same time as controls. The ethanol absorption and metabolism of the mutants is normal. However, significantly fewer DopEcR^c02142 mutants actually survive and recover from sedation.

(Petruccelli et al., 2016)

Scer\GAL4^unspecified-mediated expression of DopEcR^{dsRNA.Scer\UAS} in a DopEcR^c02142 background results in defects in the initiation of male courtship and in olfactory conditioning.

(Evans et al., 2014)

Homozygotes reach adulthood and have no gross morphological defects.

The short- and long-latency thresholds of the giant-fiber pathway in heterozygous, homozygous and DopEcR^c02142/Df(3L)ED4341 flies is indistinguishable from that of controls. However, the refractory period is significantly reduced in homozygotes compared to controls and homozygotes and DopEcR^c02142/Df(3L)ED4341 flies show a delay in habituation of the giant fiber pathway.

Homozygous and DopEcR^c02142/Df(3L)ED4341 males lack 30-minute courtship memory in a courtship conditioning assay, although homozygotes show significant memory immediately after conditioning.

(Ishimoto et al., 2013)

Unlike in wild type flies, DopEcR^c02142 mutants do not show an increased sugar sensitive proboscis extension reflex (PER) in response to either L-Dopa feeding or 6 hours of wet starvation. The loss of response to L-Dopa is observed 48 hours after feeding, whereas the starvation response is observed at 6 hours, but is not seen after 24 hours.

(Inagaki et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

DopEcR^c02142 has chemical resistant | adult stage phenotype, suppressible | partially by Egfr^KK100051/Scer\GAL4^elav.PU

(Petruccelli et al., 2016)

Suppressor of

Statement

Reference

DopEcR^c02142 is a suppressor of abnormal memory | adult stage phenotype of Pde4^dnc-1

(Ishimoto et al., 2013)

DopEcR^c02142 is a suppressor of abnormal memory | adult stage phenotype of Pde4^dnc-2

(Ishimoto et al., 2013)

NOT Suppressor of

Statement

Reference

DopEcR^c02142/DopEcR^c02142 is a non-suppressor of abnormal sleep | heat sensitive phenotype of Scer\GAL4^ple.PF, TrpA1^UAS.(B).cKa

(Liu et al., 2012)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The increased resistance to ethanol-induced sedation characteristic for DopEcR^c02142 homozygous adults is significantly suppressed by expression of Egfr^KK100051 under the control of the Scer\GAL4^elav.PU driver in the mutant background.

(Petruccelli et al., 2016)

DopEcR^c02142 restores 30-minute courtship memory in a courtship conditioning assay in dnc¹ and dnc² males.

(Ishimoto et al., 2013)

The reduction in nighttime sleep seen in flies expressing TrpA1^{Scer\UAS.(B).cKa} under the control of Scer\GAL4^ple.PF at 27[o]C is not suppressed by DopEcR^c02142/DopEcR^c02142.

(Liu et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

DopEcR^c02142 is rescued by Scer\GAL4^Mef2.247/DopEcR^UAS.cAa

(Aranda et al., 2017)

DopEcR^c02142 is rescued by DopEcR^UAS.cAa/Scer\GAL4^{αTub84B.Switch.PK}

(Aranda et al., 2017)

DopEcR^c02142 is rescued by DopEcR^UAS.cIb/Scer\GAL4^DopEcR.588

(Ishimoto et al., 2013)

DopEcR^c02142 is rescued by Scer\GAL4^c747/DopEcR^UAS.cIb

(Ishimoto et al., 2013)

DopEcR^c02142 is rescued by DopEcR^UAS.cIb/Scer\GAL4^c739

(Ishimoto et al., 2013)

DopEcR^c02142 is rescued by DopEcR^UAS.cIa/Scer\GAL4^Gr5a.8.5

(Inagaki et al., 2012)

Not rescued by

DopEcR^c02142 is not rescued by Scer\GAL4^Tab2-201Y/DopEcR^UAS.cIb

(Ishimoto et al., 2013)

Comments

DopEcR^c02142 adults show a restored ethanol-induced disinhibition after adulthood-only, but not development-only, expression of DopEcR^Scer\UAS.cAa under the control of Scer\GAL4^Mef2.247, as shown by their normal increasing frequency of inter-male courtship upon continual exposure (temporal regulation of expression is achieved by the use of Gal80[ts]). A similar rescue is achieved upon expression under the control of Scer\GAL4^{αTub84B.Switch.PK} and RU486 treatment during adulthood.

(Aranda et al., 2017)

Expression of DopEcR^Scer\UAS.cIb under the control of either Scer\GAL4^DopEcR.588, Scer\GAL4^c747 or Scer\GAL4^c739 rescues courtship memory in DopEcR^c02142 males a courtship conditioning assay.

Expression of DopEcR^Scer\UAS.cIb under the control of Scer\GAL4^Tab2-201Y does not rescue courtship memory in DopEcR^c02142 males a courtship conditioning assay.

(Ishimoto et al., 2013)

Expression of DopEcR^Scer\UAS.cIa under the control of Scer\GAL4^Gr5a.8.5 rescues the loss of the sugar-sensitive proboscis extension reflex (PER) seen in DopEcR^c02142 mutants in response to 6 hours of wet starvation.

(Inagaki et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

10847

w¹¹¹⁸; PBac{PB}DopEcR^c02142/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG18314^c02142

(Gene Disruption Project members and Exelixis, 2005)

DopEcR^PB1

(Saito et al., 2025, Petruccelli et al., 2016, Ishimoto et al., 2013)

DopEcR^c02142

(Liu et al., 2017, Petruccelli et al., 2016, Wang et al., 2016, Inagaki et al., 2012, Liu et al., 2012)

DopEcr⁰²¹⁴²

(Linford et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (13)

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