FB2026_02 , released June 18, 2026
Allele: Dmel\DopEcRGAL4
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General Information
Symbol
Dmel\DopEcRGAL4
Species
D. melanogaster
Name
FlyBase ID
FBal0323138
Feature type
allele
Associated gene
Dmel\DopEcR
Associated Insertion(s)
TI{GAL4}DopEcRGAL4
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
gene targeting by homologous recombination
(Petruccelli et al., 2016)
Progenitor genotype
Associated Insertion(s)
TI{GAL4}DopEcRGAL4
Cytology
Description

133bp of the DopEcR first coding exon ( 3L:4370598..4370730 ) has been replaced with an in-frame GAL4 coding sequence (allowing expression of the GAL4 driver under the control of endogenous DopEcR regulatory sequences) followed by a wGMR.PH marker.

(Petruccelli et al., 2016)
Allele components
Component
Use(s)
Inserted element
TI{GAL4}
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3L:4,370,598..4,370,730 [-]
Comment:

133 bp from the first exon of DopEcR were deleted and replaced with GAL4 by ends-out gene targeting.

(Petruccelli et al., 2016)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      neuron of adult brain | restricted
      (Petruccelli et al., 2016)
      adult mushroom body
      (Petruccelli et al., 2016)
      Additional Information
      Statement
      Reference

      DopEcRGAL4 drives expression in elav positive neurons in prominent neuropil structures and the mushroom bodies.

      (Petruccelli et al., 2016)
       
      Marker for
      Reflects expression of
      DopEcR
      (Petruccelli et al., 2016)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  alcohol use disorder
      CEA
      (Petruccelli et al., 2016)
      CEA with DopEcRc02142
      (Petruccelli et al., 2016)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      DopEcRc02142 homozygous and (to a lesser extent) heterozygous as well as DopEcRc02142/DopEcRGAL4 transheterozygous adults show resistance to ethanol-induced sedation: it takes significantly longer for them to become fully sedated compared to controls, although they begin to lose posture around the same time as controls, their ethanol absorption and metabolism is also normal. However, once sedated, significantly fewer DopEcRc02142 mutants survive and recover from sedation.

      (Petruccelli et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      DopEcRGAL4 has chemical resistant | adult stage phenotype, non-suppressible by DATfmn

      (Petruccelli et al., 2016)
      Suppressor of
      Statement
      Reference

      DopEcRGAL4/DopEcRGAL4 is a suppressor of chemical sensitive | adult stage | temperature conditional phenotype of mldDTS3-1

      (Petruccelli et al., 2016)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The increased resistance to ethanol-induced sedation characteristic for DopEcRGAL4 homozygous adults is strongly suppressed by expression of EgfrKK100051 driven by Scer\GAL4DopEcR-GAL4, weakly suppressed by combination with Dop1R1PL00420 and not suppressed by combination with DATfmn.

      The increased sensitivity of mldDTS3-1/+ heterozygote adults (reared at 18[o]C during development and transferred to 29[o]C after eclosion) to ethanol-induced sedation is fully suppressed by combination with DopEcRGAL4 in homozygous state. The double mutants display increased resistance to ethanol sedation which is comparable to that seen in DopEcRGAL4 mutants alone.

      (Petruccelli et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      DopEcRGAL4
      (Saito et al., 2025, Kanno et al., 2021, Kondo et al., 2020, Petruccelli et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)