FlyBase Allele Report: Dmel\Egfr[KK100051]

General Information

Symbol

Dmel\Egfr^KK100051

Species

D. melanogaster

Name

FlyBase ID

FBal0230558

Feature type

allele

Associated gene

Dmel\Egfr

Associated Insertion(s)

Carried in Construct

P{KK100051}

Also Known As

UAS-Egfr-RNAi, UAS-EGFR^RNAi, EGFR-i, UAS-EGFR dsRNA

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK100051}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK100051

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of renal fibrosis

CEA with DopEcR^KK111211

(Zheng et al., 2020)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates colorectal cancer

modeled by Src42A^CA.UAS

(Cordero et al., 2014)

exacerbates alcohol use disorder

modeled by DopEcR^c02142

(Petruccelli et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | adult stage, with Scer\GAL4^NP0001

(Socha et al., 2023)

chemical sensitive | adult stage, with Scer\GAL4^elav.PU

(Petruccelli et al., 2016)

increased cell death | heat sensitive, with Scer\GAL4^wg-IS650, Scer\GAL80^ts.αTub84B

(Takemura and Adachi-Yamada, 2011)

Phenotype Manifest In

adult enterocyte | conditional, with Scer\GAL4^NP0001

(Socha et al., 2023)

crossvein, with Scer\GAL4^en-e16E

(Hahn et al., 2013)

wing, with Scer\GAL4^en-e16E

(Hahn et al., 2013)

wing margin | heat sensitive, with Scer\GAL4^wg-IS650, Scer\GAL80^ts.αTub84B

(Takemura and Adachi-Yamada, 2011)

wing vein, with Scer\GAL4^en-e16E

(Hahn et al., 2013)

wing vein L4, with Scer\GAL4^en-e16E

(Hahn et al., 2013)

Detailed Description

Statement

Reference

Expression of Egfr^KK100051 under the control of Scer\GAL4^elav.PU leads to more rapid ethanol sedation compared to controls.

(Petruccelli et al., 2016)

Reducing Egfr levels by Egfr^KK100051-mediated knockdown in the posterior wing compartment (under the control of Scer\GAL4^en-e16E), leads to the loss of the lateral vein L4 and the apical crossvein, along with some growth defects.

(Hahn et al., 2013)

Knockdown of Egfr in wing margin cells, through the expression of Egfr^KK100051 under the control of Scer\GAL4^wg-IS650 and Scer\GAL80^ts.αTub84B results in extra apoptosis even at 30 hours after pupal formation. As a result of this apoptosis, some inter-hair cells are lost.

(Takemura and Adachi-Yamada, 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Egfr^KK100051/Scer\GAL4^elav.PU is a suppressor | partially of chemical resistant | adult stage phenotype of DopEcR^c02142

(Petruccelli et al., 2016)

Egfr^KK100051/Scer\GAL4^DopEcR-GAL4 is a suppressor | partially of chemical resistant | adult stage phenotype of DopEcR^GAL4

(Petruccelli et al., 2016)

Egfr^KK100051, Scer\GAL4^NP5130 is a suppressor of hyperplasia phenotype of Scer\GAL4^NP5130, Src42A^CA.UAS

(Cordero et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Egfr^KK100051, Scer\GAL4^en-e16E has wing vein L4 phenotype, enhanceable by step^RNAi.UAS, Scer\GAL4^en-e16E

(Hahn et al., 2013)

Egfr^KK100051, Scer\GAL4^en-e16E has crossvein phenotype, enhanceable by step^RNAi.UAS, Scer\GAL4^en-e16E

(Hahn et al., 2013)

Egfr^KK100051, Scer\GAL4^en-e16E has wing phenotype, enhanceable by step^RNAi.UAS, Scer\GAL4^en-e16E

(Hahn et al., 2013)

Egfr^KK100051, Scer\GAL4^en-e16E has wing vein phenotype, enhanceable by step^RNAi.UAS, Scer\GAL4^en-e16E

(Hahn et al., 2013)

Suppressed by

Statement

Reference

Egfr^KK100051, Scer\GAL4^en-e16E has wing vein L4 phenotype, suppressible by step^UAS.FL, Scer\GAL4^en-e16E

(Hahn et al., 2013)

Egfr^KK100051, Scer\GAL4^en-e16E has crossvein phenotype, suppressible by step^UAS.FL, Scer\GAL4^en-e16E

(Hahn et al., 2013)

Egfr^KK100051, Scer\GAL4^en-e16E has wing phenotype, suppressible by step^UAS.FL, Scer\GAL4^en-e16E

(Hahn et al., 2013)

Egfr^KK100051, Scer\GAL4^en-e16E has wing vein phenotype, suppressible by step^UAS.FL, Scer\GAL4^en-e16E

(Hahn et al., 2013)

Suppressor of

Statement

Reference

Egfr^KK100051, Scer\GAL4^NP5130 is a suppressor of intestinal stem cell of posterior adult midgut epithelium phenotype of Scer\GAL4^NP5130, Src42A^CA.UAS

(Cordero et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The increased resistance to ethanol-induced sedation characteristic for both DopEcR^c02142 and DopEcR^GAL4 homozygous adults is significantly suppressed by expression of Egfr^KK100051 under the control of the Scer\GAL4^elav.PU driver in the DopEcR^c02142 background or the intrinsic Scer\GAL4^DopEcR-GAL4 driver in the DopEcR^GAL4 mutants.

(Petruccelli et al., 2016)

Reducing Egfr levels by Egfr^KK100051-mediated knockdown in the posterior wing compartment (under the control of Scer\GAL4^en-e16E), in a step^{dsRNA.Scer\UAS} background generates a more severe Egfr phenotype. The lateral vein L4 is entirely lost, both cross veins are not differentiated and the distal end of the lateral vein L5 is missing.

Expression of step^Scer\UAS.FL suppresses the wing vein defects caused by expression of Egfr^KK100051 in the posterior compartment of the wing, under the control of Scer\GAL4^en-e16E.

(Hahn et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v107130

P{KK100051}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Egfr^KK100051

Name Synonyms

Secondary FlyBase IDs

References (22)

Report Sections

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