FlyBase Allele Report: Dmel\Surf1[RNAi.UAS]

General Information

Symbol

Dmel\Surf1^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0191505

Feature type

allele

Associated gene

Dmel\Surf1

Associated Insertion(s)

Carried in Construct

P{UAS-Surf1.RNAi}

Also Known As

UAS-Surf1-IR, Surf1-KD

Key Links

Transgenic product class

RNAi_reagent

(Zordan et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Zordan et al., 2006)

Mutagen

in vitro construct

(Zordan et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-Surf1.RNAi}

Cytology

Description

UASt regulatory sequences drive expression of two copies of a Surf1 fragment in an inverted repeat, which is separated by a spacer sequence (330bp fragment of the GFP coding sequence).

(Zordan et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Surf1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Leigh disease

CEA

(Da-Rè et al., 2014)

model of mitochondrial complex IV deficiency nuclear type 1

CEC

(Cagin et al., 2015)

CEA

(Duncan et al., 2018)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of mitochondrial complex IV deficiency nuclear type 1

is ameliorated by aop^NIG.3166R

(Duncan et al., 2018)

is ameliorated by pnt^Δ88

(Duncan et al., 2018)

is ameliorated by pnt^JF02227

(Duncan et al., 2018)

model of Leigh disease

is ameliorated by sima^HMS00833

(Cagin et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | larval stage, with Scer\GAL4^Act5C.PI

(Zordan et al., 2006)

abnormal locomotor behavior | adult stage, with Scer\GAL4^nSyb.PU

(Cagin et al., 2015)

abnormal locomotor behavior | larval stage, with Scer\GAL4^Act5C.PI

(Zordan et al., 2006)

abnormal neurophysiology, with Scer\GAL4^elav.PLu

(Zordan et al., 2006)

abnormal optomotor response, with Scer\GAL4^elav.PLu

(Zordan et al., 2006)

abnormal phototaxis | larval stage, with Scer\GAL4^Act5C.PI

(Zordan et al., 2006)

decreased body size | larval stage, with Scer\GAL4^Act5C.PI

(Zordan et al., 2006)

decreased body size | third instar larval stage, with Scer\GAL4^Act5C.PU

(Da-Rè et al., 2014)

lethal | RU486 conditional, with Scer\GAL4^{Act5C.Switch.PR}

(Da-Rè et al., 2014)

lethal | RU486 conditional, with Scer\GAL4^βTub.Switch

(Fernandez-Ayala et al., 2009)

lethal - all die before end of P-stage, with Scer\GAL4^how-24B

(Da-Rè et al., 2014)

lethal - all die during larval stage, with Scer\GAL4^Act5C.PI

(Zordan et al., 2006)

lethal - all die during larval stage, with Scer\GAL4^Act5C.PU

(Da-Rè et al., 2014)

long lived, with Scer\GAL4^elav.PLu

(Zordan et al., 2006)

partially lethal - majority die | RU486 conditional, with Scer\GAL4^{αTub84B.Switch.PK}

(Kemppainen et al., 2014)

some die during larval stage, with Scer\GAL4^how-24B

(Da-Rè et al., 2014)

some die during P-stage, with Scer\GAL4^Act5C.PI

(Zordan et al., 2006)

some die during P-stage, with Scer\GAL4^how-24B

(Da-Rè et al., 2014)

visible | adult stage, with Scer\GAL4^nSyb.PU

(Cagin et al., 2015)

Phenotype Manifest In

embryonic/larval optic lobe, with Scer\GAL4^Act5C.PI

(Zordan et al., 2006)

larval somatic muscle cell | larval stage, with Scer\GAL4^how-24B

(Da-Rè et al., 2014)

mitochondrion, with Scer\GAL4^Act5C.PI

(Zordan et al., 2006)

mitochondrion, with Scer\GAL4^how-24B

(Da-Rè et al., 2014)

postsynaptic membrane, with Scer\GAL4^Act5C.PI

(Zordan et al., 2006)

wing blade, with Scer\GAL4^nSyb.PU

(Cagin et al., 2015)

Detailed Description

Statement

Reference

Expression of Surf1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^nSyb.PU results in strongly diminished climbing ability in adult flies - more so in males than in females and majority of the adults show wing inflation defects.

(Cagin et al., 2015)

Animals expressing Surf1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^how-24B during the early stages of pupal metamorphosis, mostly dying at early imago stages, when adult cuticular structures have only just everted. Apolysis does not occur correctly and larval cuticular structures, such as mouth hooks, remain in the pupa. The gas bubble is not displaced to the anterior region of the puparium and the head does not evert. Adult muscle does not develop in the mutant pupae.

Muscles in larvae expressing Surf1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^how-24B show impaired development and vacuolisation and a specific longitudinal fibre in abdominal segment 4 is missing. The muscle nuclei have an abnormal 'coffee bean' shaped morphology. The mitochondria in the muscles are enlarged and rounded and their internal structure is often disorganised or disrupted. mtDNA copy number appears close to controls. The resting potential of the mutant mitochondria is normal, but they show a clear decrease in the rate of Ca[2+] uptake compared to controls.

Expression of Surf1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^{Act5C.Switch.PR} in the presence of RU486 in adults results in 100% lethality within 48 hours.

Animals in which Surf1^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^{Act5C.Switch.PR} in the presence of RU486 in the third larval instar reach the pupal stage but fail to develop into adults.

(Da-Rè et al., 2014)

Expression of Surf1^{dsRNA.Scer\UAS} driven by Scer\GAL4^{αTub84B.Switch.PK} results in dosage-dependent lethality: 0.5uM RU486 dosage prevents pupariation, and 0.1uM results in very few eclosing flies.

(Kemppainen et al., 2014)

Most individuals that express Surf1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PI die as second or third instar larvae, depending on the insertion of Surf1^{dsRNA.Scer\UAS}. Only a few individuals reach the pupal stage and all of these die at early imago stages. Scer\GAL4^Act5C.PI>Surf1^{dsRNA.Scer\UAS} larvae are sluggish, show impaired development and have undersized optic lobes. Scer\GAL4^Act5C.PI>Surf1^{dsRNA.Scer\UAS} larvae show a highly significant reduction in locomotor speed and a reduced photobehavioural response. This altered locomotion is not due to muscular structural defects as muscle fibers are morphologically normal. Physiological tests show that muscular impairments involving signal transduction, calcium release, and contractile abnormalities are not responsible for the observed phenotypes. Examination of the neuromuscular junction of muscle fibers 6 and 7 reveals no abnormality in the number, size or type of synaptic bouton. However, the structure of the subsynaptic reticulum has a lower complexity than wild type. No abnormalities in the evoked junction potentials are observed. Mitochondria in the muscle tissue of Scer\GAL4^Act5C.PI>Surf1^{dsRNA.Scer\UAS} larvae are larger than wild type and more rounded in shape. Unlike wild-type mitochondria, Surf1^{dsRNA.Scer\UAS}-overexpressing mitochondria do not appear to be restricted to the intermyofibrillar spaces, and do not show a tendency to cluster. Flies expressing Surf1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav.PLu show a significant increase in lifespan. These flies show no evidence of age-dependent neurodegeneration and no abnormalities in evoked junction potentials. The majority of Scer\GAL4^elav.PLu>Surf1^{dsRNA.Scer\UAS} flies (the exception is for females carrying the P{UAS-Surf1.dsRNA}79.10 insertion) show an impaired capacity to react to the rotating environment in optomoter tests, but as all of the flies show movement, they do not suffer gross motor impairments. ERG recordings from Scer\GAL4^elav.PLu>Surf1^{dsRNA.Scer\UAS} flies show reduced or absent ON/OFF transients, meaning that these flies have a defect in the synaptic activation of second-order neurons of the visual pathway. Only flies carrying the P{UAS-Surf1.dsRNA}79.1 insertion, when expression of the inserted transgene is driven by Scer\GAL4^elav.PLu, show an increased sustained response, which is due to photoreceptor light-induced polarization.

(Zordan et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^nSyb.PU, Surf1^RNAi.UAS has abnormal locomotor behavior | female | adult stage phenotype, suppressible by Scer\GAL4^nSyb.PU/sima^HMS00833

(Cagin et al., 2015)

Scer\GAL4^nSyb.PU, Surf1^RNAi.UAS has abnormal locomotor behavior | male | adult stage phenotype, suppressible | partially by Scer\GAL4^nSyb.PU/sima^HMS00833

(Cagin et al., 2015)

Scer\GAL4^nSyb.PU, Surf1^RNAi.UAS has visible | adult stage phenotype, suppressible by Scer\GAL4^nSyb.PU/sima^HMS00833

(Cagin et al., 2015)

Scer\GAL4^{αTub84B.Switch.PK}, Surf1^RNAi.UAS has partially lethal - majority die | RU486 conditional phenotype, suppressible | partially by Cint\AOX^αTub84B.PK

(Kemppainen et al., 2014)

Scer\GAL4^βTub.Switch, Surf1^RNAi.UAS has lethal | RU486 conditional phenotype, suppressible | RU486 conditional by Cint\AOX^UAS.cFa, Scer\GAL4^βTub.Switch

(Fernandez-Ayala et al., 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^nSyb.PU, Surf1^RNAi.UAS has wing blade phenotype, suppressible by Scer\GAL4^nSyb.PU/sima^HMS00833

(Cagin et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The reduced climbing ability as well as the wing inflation defects characteristic for adult flies expressing Surf1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^nSyb.PU are significantly suppressed by co-expression of sima^HMS00833.

(Cagin et al., 2015)

Xenogenetic Interactions

Statement

Reference

Zzzz\AOX^αTub84B.PK significantly suppresses dosage-dependent lethality (with 0.1 to 0.5uM RU486) in flies with expression of Surf1^{dsRNA.Scer\UAS} driven by Scer\GAL4^{αTub84B.Switch.PK}.

(Kemppainen et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Transfected into Drosophila and synthesised into dsRNA in vivo to study the phenotypic consequences of dsRNA interference (RNAi) of the Surf1 gene.

(Zordan et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Surf1^RNAi.UAS

Surf1^{dsRNA.Scer\UAS}

Surf1^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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