FlyBase Allele Report: Dmel\pnt[JF02227]

General Information

Symbol

Dmel\pnt^JF02227

Species

D. melanogaster

Name

FlyBase ID

FBal0244904

Feature type

allele

Associated gene

Dmel\pnt

Associated Insertion(s)

Carried in Construct

P{TRiP.JF02227}

Also Known As

UAS-pnt RNAi, UAS-pnt-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.JF02227}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF02227

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates mitochondrial DNA depletion syndrome 15

modeled by TFAM^UAS.cCa

(Duncan et al., 2018)

ameliorates mitochondrial complex IV deficiency nuclear type 1

modeled by Surf1^RNAi.UAS

(Duncan et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^pnt-14-94

(Xie et al., 2016)

decreased cell number | third instar larval stage, with Scer\GAL4^pnt-14-94

(Xie et al., 2016)

increased cell number | third instar larval stage, with Scer\GAL4^pnt-14-94

(Xie et al., 2016)

Phenotype Manifest In

intermediate neuronal progenitor | third instar larval stage, with Scer\GAL4^pnt-14-94

(Xie et al., 2016)

type II neuroblast | third instar larval stage, with Scer\GAL4^pnt-14-94

(Xie et al., 2016)

Detailed Description

Statement

Reference

Expression of pnt^JF02227 under the control of Scer\GAL4^pnt-14-94 (in combination with a Dicer-2 transgene to enhance RNAi efficiency) results in a significant increase in type II neuroblasts and complete elimination of intermediate neural precursors in third instar larval brains, as compared to controls.

(Xie et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^pnt-14-94, pnt^JF02227 has increased cell number | third instar larval stage phenotype, enhanceable by Df(2L)erm²/+

(Xie et al., 2016)

Scer\GAL4^pnt-14-94, pnt^JF02227 has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by Df(2L)erm²/+

(Xie et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^pnt-14-94, pnt^JF02227 has type II neuroblast | third instar larval stage phenotype, enhanceable by Df(2L)erm²/+

(Xie et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Df(2L)erm²/+ significantly enhances the increase in type II neuroblasts seen in larvae expressing pnt^JF02227 under the control of Scer\GAL4^pnt-14-94.

(Xie et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

31936

y¹ v¹; P{TRiP.JF02227}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

pnt^JF02227

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

Open Close