FlyBase Allele Report: Dmel\COX5A[GD5167]

General Information

Symbol

Dmel\COX5A^GD5167

Species

D. melanogaster

Name

FlyBase ID

FBal0206598

Feature type

allele

Associated gene

Dmel\COX5A

Associated Insertion(s)

Carried in Construct

P{GD5167}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD5167}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD5167

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cytochrome-c oxidase deficiency disease

CEA

(Kemppainen et al., 2014)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of cytochrome-c oxidase deficiency disease

is ameliorated by Zzzz\AOX^αTub84B.PK

(Kemppainen et al., 2014)

is ameliorated by Cint\AOX^αTub84B.PK

(Kemppainen et al., 2014)

exacerbates cancer

modeled by Wwox^KK108459

(Choo et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

ommatidium, with Scer\GAL4^ey.PU

(Choo et al., 2015)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

The expression of COX5A^GD5167 under the control of Scer\GAL4^ey.PU induces disruption to the patterning of ommatidia.

(Choo et al., 2015)

Expression of COX5A^GD5167 driven by Scer\GAL4^{αTub84B.Switch.PK} results in dosage-dependent lethality: 0.5uM RU486 results in zero eclosing flies, with survival increasing as dosage is decreased to 0.1uM.

(Kemppainen et al., 2014)

Adults expressing COX5A^GD5167 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in early larval lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in wild-type sarcomeres in the larval body wall muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 20-30% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 50-60% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 20-30% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 50-60% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

5167

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

COX5A^GD5167, Scer\GAL4^{αTub84B.Switch.PK} has partially lethal - majority die | RU486 conditional phenotype, suppressible | partially by Cint\AOX^αTub84B.PK/Zzzz\AOX[+]

(Kemppainen et al., 2014)

COX5A^GD5167, Scer\GAL4^{αTub84B.Switch.PK} has partially lethal - majority die | RU486 conditional phenotype, suppressible | partially by Cint\AOX^αTub84B.PK/Cint\AOX^αTub84B.PK

(Kemppainen et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

COX5A^GD5167, Scer\GAL4^ey.PU has eye phenotype, enhanceable by Wwox^KK108459, Scer\GAL4^ey.PU

(Choo et al., 2015)

Suppressed by

Statement

Reference

COX5A^GD5167, Scer\GAL4^ey.PU has eye phenotype, suppressible | partially by Wwox^UAS.cCa, Scer\GAL4^ey.PU

(Choo et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Zzzz\AOX^αTub84B.PK significantly partially suppresses dosage-dependent lethality (with 0.1 to 0.5uM RU486) in flies with expression of COX5A^GD5167 driven by Scer\GAL4^{αTub84B.Switch.PK}; further suppression is seen with two copies of Zzzz\AOX^αTub84B.PK.

(Kemppainen et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v44490

w¹¹¹⁸; P{GD5167}v44490

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

5167

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

COX5A^GD5167

CoVa^GD5167

CoVa^RNAi

(Choo et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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