UASt regulatory sequences drive expression of an inverted repeat.
Expression of cypeGD908 driven by Scer\GAL4αTub84B.Switch.PK (with 10uM RU486) results in lethality of about 75% of progeny and flies that eclose do so with a 3-day delay (RU486 dose-dependent), compared to controls (on 1uM RU486).
Adults expressing cypeGD908 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) can show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies, depending on the particular P{GD908} insertion line used.
Expression of cypeGD908 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD908} insertion line used.
Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in adult/pharate lethality or late pupal lethality.
Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 80-90% or 90-100% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 results in the absence of 0% or 20-30% of the Scer\GAL4pnr-MD237-expressing area of the notum, depending on the insertion line used.
Scer\GAL4αTub84B.Switch.PK, cypeGD908 has partially lethal - majority die | RU486 conditional phenotype, suppressible | partially by Cint\AOXαTub84B.PK
Scer\GAL4αTub84B.Switch.PK, cypeGD908 has abnormal developmental rate | RU486 conditional phenotype, suppressible | partially by Cint\AOXαTub84B.PK
Scer\GAL4da.G32, cypeGD908 has partially lethal - majority die phenotype, suppressible by Cint\AOXUAS.cFa, Scer\GAL4da.G32
Zzzz\AOXαTub84B.PK significantly suppresses lethality and delays in development seen in flies with expression of cypeGD908 driven by Scer\GAL4αTub84B.Switch.PK (with RU486).