FlyBase Allele Report: Dmel\Raf[F22.hs]

General Information

Symbol

Dmel\Raf^F22.hs

Species

D. melanogaster

Name

FlyBase ID

FBal0039752

Feature type

allele

Associated gene

Dmel\Raf

Associated Insertion(s)

Carried in Construct

P{Hsp70-Draf.gof}

Also Known As

hsRaf^*M7

Key Links

Transgenic product class

gain_of_function_variant

(Brand and Perrimon, 1994)

inframe_deletion

(Brand and Perrimon, 1994)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

gain_of_function_variant

(Brand and Perrimon, 1994)

inframe_deletion

(Brand and Perrimon, 1994)

Mutagen

in vitro construct

(Brand and Perrimon, 1994)

Progenitor genotype

Carried in construct

P{Hsp70-Draf.gof}

Cytology

Description

An Hsp70 promoter drives expression of the Raf coding sequence in which amino acid residues 2-431 have been deleted.

(Brand and Perrimon, 1994)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Raf

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA

(Zhao et al., 2021)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of cancer

is ameliorated by slif^UY681

(Zhao et al., 2021)

is ameliorated by Atg1^HMS02750

(Zhao et al., 2021)

is exacerbated by Atg101^6h

(Zhao et al., 2021)

is ameliorated by Atg16^MI00187

(Zhao et al., 2021)

is ameliorated by Atg5^HMS01244

(Zhao et al., 2021)

is ameliorated by Atg6^HMS01483

(Zhao et al., 2021)

is ameliorated by Balat^HMC06377

(Zhao et al., 2021)

is ameliorated by CarT^HMC04919

(Zhao et al., 2021)

is ameliorated by Cat^UAS.cAa

(Zhao et al., 2021)

is ameliorated by bsk^HMS00777

(Zhao et al., 2021)

is ameliorated by bsk^K53R.UAS

(Zhao et al., 2021)

is ameliorated by slif^HMC05846

(Zhao et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death

(Kurada and White, 1998)

lethal - all die during embryonic stage | maternal effect | heat sensitive

(Brand and Perrimon, 1994)

some die during embryonic stage | maternal effect | heat sensitive

(Brand and Perrimon, 1994)

visible | heat sensitive

(Martin-Blanco et al., 1999)

Phenotype Manifest In

Detailed Description

Statement

Reference

phl^F22.hs heterozygous flies maintained at 25[o]C exhibit normal life spans and locomotor activity.

Overexpression of phl^F22.hs does not affect superoxide production.

(Tong et al., 2007)

16.6% of eggs derived from females expressing one copy of phl^F22.hs using heat shock are dorsalised, with dorsal appendage material being expanded throughout the anterior of the egg. The penetrance of the dorsalised egg phenotype is 61.7% if the females express two copies of phl^F22.hs.

(Hsu et al., 2001)

Expression of phl^F22.hs during the late third larval instar stage using heat shock results in flies that show a significant amount of wing vein loss. Heat shock during the early-to-mid second larval instar stages results in flies with ectopic wing vein tissue, in most cases in close proximity to a vein.

(Martin-Blanco et al., 1999)

Ubiquitous expression in the embryo weakly suppresses normal cell death.

(Kurada and White, 1998)

Modulation of voltage gated K⁺ currents induced by the neuropeptide pituitary adenylyl cyclase-activating polypeptide (PACAP38) is eliminated. The mutation fails to block or mimic PACAP38 (neuropeptide pituitary adenylyl cyclase-activating polypeptide) induced enhancement of K⁺ currents.

(Guo et al., 1997)

Expression of an activated phl mutation has no effect on size of Nf1 mutant pupae.

(The et al., 1997)

After 3 days of heat shock homozygous females lay dorsalised embryos, dorsal appendages are fused in a ring at the anterior end and eggs are smaller and more spherical than wild type (comparable to eggs laid by fs(1)K10¹ females). Some embryos exhibit a more severe phenotype. Expression of Hsap\RAF1^gof.hs leads to more embryos exhibiting the stronger chorion phenotype.

(Brand and Perrimon, 1994)

Females lay eggs with a dorsalised chorion.

(Hsu and Perrimon, 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Raf^F22.hs is a non-suppressor of abnormal locomotor rhythm phenotype of Nf1^P1

(Williams et al., 2001)

Phenotype Manifest In

Enhanced by

Statement

Reference

Raf^F22.hs has dorsal appendage phenotype, enhanceable by dod^hs.PH

(Hsu et al., 2001)

Suppressed by

Statement

Reference

Raf^F22.hs has egg chorion phenotype, suppressible by Dsor1^+t9

(Hsu and Perrimon, 1994)

Additional Comments

Genetic Interactions

Statement

Reference

The penetrance of the dorsalised egg phenotype produced by females expressing one copy of phl^F22.hs is enhanced (to 56.8%) if the females are also expressing dod^hs.PH.

(Hsu et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8497

w^*; P{Hsp70-Draf.gof}3/TM3, Sb¹

Notes on Origin

Discoverer

A.H. Brand and N. Perrimon.

(Hsu and Perrimon, 1994)

Comments

Constitutively active form of phl.

(Brand and Perrimon, 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

DrafΔN

(Li et al., 1998)

Raf^F22.hs

Raf^gof

(Guo et al., 1997)

Ras^gof

(The et al., 1997)

hsΔDraf²²

phl^F22.hs

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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