FlyBase Allele Report: Dmel\Atg5[HMS01244]

General Information

Symbol

Dmel\Atg5^HMS01244

Species

D. melanogaster

Name

FlyBase ID

FBal0263257

Feature type

allele

Associated gene

Dmel\Atg5

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01244}

Also Known As

UAS-Atg5^RNAi, Atg5-RNAi, UAS-Atg5 RNAi, Atg5 RNAi, atg5^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.HMS01244}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01244

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by Raf^F22.hs

(Zhao et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^alrm.PD

(Damulewicz et al., 2022)

abnormal neuroanatomy | adult stage, with Scer\GAL4^repo.PU

(Damulewicz et al., 2022)

abnormal sleep | adult stage, with Scer\GAL4^alrm.PD

(Damulewicz et al., 2022)

abnormal starvation stress response | adult stage, with Scer\GAL4^repo

(Leung et al., 2024)

abnormal wound healing | adult stage, with Scer\GAL4^repo

(Szabó et al., 2023)

decreased size | adult stage, with Scer\GAL4^en-e16E

(Tyra et al., 2020)

decreased size | adult stage, with Scer\GAL4^nub-AC-62

(Tyra et al., 2020)

increased mortality, with Scer\GAL4^repo

(Leung et al., 2024)

increased occurrence of cell division | adult stage, with Scer\GAL4^esg.PU

(Zhang et al., 2019)

increased sleep | adult stage, with Scer\GAL4^alrm.PD

(Damulewicz et al., 2022)

increased sleep | adult stage, with Scer\GAL4^repo.PU

(Damulewicz et al., 2022)

visible | adult stage, with Scer\GAL4^en-e16E

(Tyra et al., 2020)

visible | adult stage, with Scer\GAL4^nub-AC-62

(Tyra et al., 2020)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adulthood-only expression of Atg5^HMS01244 under the control of Scer\GAL4^esg.PU leads to a significant increase in the number of mitotic cells (phospho-H3 staining) in the adult midgut, as compared to controls. (The temporal regulation of expression is dependent on Gal80[ts] and induced by temperature shift to 29[o]C for 5-6 days during adulthood).

(Zhang et al., 2019)

Expression of Atg5^HMS01244 under the control of Scer\GAL4^Mef2.PR is able to significantly suppress the formation of autophagosomes in the muscles of chloroquine-treated larvae starved on low-nutrient food for 6 hours.

(Zirin et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Scer\GAL4^Act5C.PI/Atg5^HMS01244 is a non-enhancer of decreased cell number | adult stage | somatic clone phenotype of Phb1²⁰

(Zhao et al., 2022)

Scer\GAL4^Act5C.PI/Atg5^HMS01244 is a non-enhancer of decreased cell number | adult stage | somatic clone phenotype of Phb2^Δ