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General Information
Symbol
Dmel\Pink1UAS.cYa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Yang
FlyBase ID
FBal0239191
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-dPink1
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of Pink1 coding sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Ectopic expression of Pink1Scer\UAS.cYa under the control of Scer\GAL4ple.PF has no significant effect on the number of dopaminergic PPL1 neurons in the adult brain.

Expression of Pink1Scer\UAS.cYa under the control of Scer\GAL4ple.PF in larval motor neurons results in decreased axonal mitochondrial transport flux and velocity in both anterograde and retrograde directions, decreased mitochondrial length, and decreased accumulation of mitochondria in the most distal boutons of neuromuscular junctions, as compared with controls.

Mitochondrial aggregates form in the dorsolateral protocerebral posterior DA (dopaminergic) neuron cluster in animals expressing Pink1Scer\UAS.cYa under the control of Scer\GAL4ple.PF. The nonaggregated mitochondria appear spherical and tubular mitochondrial structures are rarely seen.

The fraction of cultured dopaminergic neurons having thread-like tubular mitochondria is lower in cells expressing Pink1Scer\UAS.cYa under the control of Scer\GAL4ple.PF than in control cells.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT suppressed by
Statement
Reference

Pink1UAS.cYa, Scer\GAL4ple.PF/Scer\GAL4ple.PF has mitochondrion & dorso-lateral dopaminergic neuron phenotype, non-suppressible by Drp1Tag:FLAG,Tag:CALI(TC),Tag:HA

Pink1UAS.cYa, Scer\GAL4ple.PF/Scer\GAL4ple.PF has mitochondrion & dorso-lateral dopaminergic neuron phenotype, non-suppressible by opa1-like[+]/Opa1EY09863

Pink1UAS.cYa, Scer\GAL4ple.PF/Scer\GAL4ple.PF has mitochondrion & dorso-lateral dopaminergic neuron phenotype, non-suppressible by parkUAS.Tag:HA, Scer\GAL4ple.PF/Scer\GAL4ple.PF

Enhancer of
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The loss of dopaminergic (DA) PPL1 neurons along with the mitochondrial aggregations in DA neurons observed in the brains of adult flies expressing Tom20KK107626 RNAi under the control of Scer\GAL4ple.PF are partially suppressed by co-expression of Pink1Scer\UAS.cYa but this does not affect the loss of PPL1 neurons induced by expression of either Tim8JF01533 or Tom40JF02030. The wing posture and jumping ability defects characteristic for adult flies expressing pumScer\UAS.cYa controlled by the Scer\GAL4Mhc.PU driver are also not rescued by co-expression of Pink1Scer\UAS.cYa.

Co-expression of Pink1Scer\UAS.cYa enhances the increase in the number of type II neuroblasts which is seen in the central brain of animals expressing NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4pnt-14-94.

The mitochondrial aggregation phenotype seen in the dorsolateral protocerebral posterior DA (dopaminergic) neuron cluster in animals expressing Pink1Scer\UAS.cYa under the control of Scer\GAL4ple.PF is not modified by Drp1T:Zzzz\FLAG,T:Zzzz\TC,T:Ivir\HA1 or by opa1-likeEY09863/+.

The mitochondrial aggregation phenotype seen in the dorsolateral protocerebral posterior DA (dopaminergic) neuron cluster in animals expressing Pink1Scer\UAS.cYa under the control of Scer\GAL4ple.PF is not suppressed by co-expression of parkScer\UAS.T:Ivir\HA1.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The expression of Pink1Scer\UAS.cYa, alongside Pink1dsRNA.Scer\UAS, both under the control of Scer\GAL4Mhc.PW, rescues the tissue-specific RNAi phenotype.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Pink1Scer\UAS.cYa
Pink1UAS.cYa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Yang
Secondary FlyBase IDs
    References (9)