FlyBase Allele Report: Dmel\pum[UAS.cYa]

General Information

Symbol

Dmel\pum^UAS.cYa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Ye

FlyBase ID

FBal0154719

Feature type

allele

Associated gene

Dmel\pum

Associated Insertion(s)

Carried in Construct

P{UAS-pum.Y}

Also Known As

UAS-pum

Key Links

Transgenic product class

cDNA

(Ye et al., 2004)

wild_type

(Ye et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Ye et al., 2004)

wild_type

(Ye et al., 2004)

Mutagen

in vitro construct

(Ye et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-pum.Y}

Cytology

Description

UASt sequences drive expression of pum cDNA sequences.

(Ye et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

pum

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal jumping, with Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

abnormal neuroanatomy, with Scer\GAL4^5-78

(Ye et al., 2004)

abnormal neuroanatomy, with Scer\GAL4⁴⁷⁷

(Ye et al., 2004)

abnormal neuroanatomy, with Scer\GAL4^ppk.PG

(Lin et al., 2015)

abnormal neuroanatomy | adult stage, with Scer\GAL4^ple.PF

(Gehrke et al., 2015)

decreased cell number | adult stage, with Scer\GAL4^ple.PF

(Gehrke et al., 2015)

lethal, with Scer\GAL4^109(2)80

(Ye et al., 2004)

visible | adult stage | progressive, with Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

Phenotype Manifest In

dendritic arborising neuron & dendritic tree, with Scer\GAL4^5-78

(Ye et al., 2004)

dendritic arborising neuron & dendritic tree, with Scer\GAL4⁴⁷⁷

(Ye et al., 2004)

dopaminergic neuron | adult stage, with Scer\GAL4^ple.PF

(Gehrke et al., 2015)

dopaminergic PPL1 neuron | adult stage, with Scer\GAL4^ple.PF

(Gehrke et al., 2015)

flight muscle cell | adult stage, with Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

larval multidendritic class IV neuron, with Scer\GAL4^ppk.PG

(Lin et al., 2015)

mitochondrion | adult stage, with Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

mitochondrion | adult stage, with Scer\GAL4^ple.PF

(Gehrke et al., 2015)

wing | adult stage | progressive, with Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

Detailed Description

Statement

Reference

Expression of pum^Scer\UAS.cYa under the control of Scer\GAL4^Mhc.PU results in wing posture (age-progressive), jumping ability defects and abnormal mitochondrial morphology in flight muscles in adult flies, while expression under Scer\GAL4^ple.PF leads to loss of dopaminergic (DA) neurons from the PPL1 cluster and mitochondrial aggregation in the DA neurons.

(Gehrke et al., 2015)

Scer\GAL4^ppk.PG-mediated overexpression of pum^Scer\UAS.cYa strongly attenuates C4da terminal dendrite growth in an otherwise wild-type background.

(Lin et al., 2015)

Expression of pum^Scer\UAS.cYa under the control of Scer\GAL4^5-78 results in a change in the dendrite morphology of class III dendritic arborization neurons of third instar larvae. Expression of pum^Scer\UAS.cYa under the control of Scer\GAL4⁴⁷⁷ results in a change in the dendrite morphology of class IV dendritic arborization neurons of third instar larvae.

(Ye et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^Mhc.PU, pum^UAS.cYa has visible | adult stage | progressive phenotype, non-suppressible by Pink1^UAS.cYa, Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

Scer\GAL4^Mhc.PU, pum^UAS.cYa has abnormal jumping phenotype, non-suppressible by Pink1^UAS.cYa, Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

NOT Enhancer of

Statement

Reference

Scer\GAL4^ppk.PG/pum^UAS.cYa is a non-enhancer of abnormal neuroanatomy | larval stage phenotype of path^dg50/Df(3L)BSC773

(Lin et al., 2015)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^Mhc.PU, pum^UAS.cYa has wing | adult stage | progressive phenotype, non-suppressible by Pink1^UAS.cYa, Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

NOT Enhancer of

Statement

Reference

Scer\GAL4^ppk.PG/pum^UAS.cYa is a non-enhancer of larval multidendritic class IV neuron phenotype of path^dg50/Df(3L)BSC773

(Lin et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The wing posture and jumping ability defects characteristic for adult flies expressing pum^Scer\UAS.cYa under the control of Scer\GAL4^Mhc.PU are not rescued by co-expression of Pink1^Scer\UAS.cYa.

(Gehrke et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

pum^Scer\UAS.cYa

pum^UAS.cYa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Ye

(Ye et al., 2004)

Secondary FlyBase IDs

References (5)

Report Sections

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