FlyBase Allele Report: Dmel\Dronc[C318G.UAS.EGFP]

General Information

Symbol

Dmel\Dronc^{C318G.UAS.EGFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0122050

Feature type

allele

Associated gene

Dmel\Dronc

Associated Insertion(s)

Carried in Construct

P{UAS-Dronc.EGFP.C318G}

Also Known As

UAS-Dronc^DN

Key Links

Transgenic product class

missense_variant

(Quinn et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Quinn et al., 2000)

Mutagen

in vitro construct

(Quinn et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-Dronc.EGFP.C318G}

Cytology

Description

UASt regulatory sequences drive expression of an inactive form of Dronc tagged with EGFP.

(Quinn et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Dronc

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4⁴⁷⁷

(Williams et al., 2006)

visible | adult stage, with Scer\GAL4^ap-md544

(Shinoda et al., 2019)

Phenotype Manifest In

dendrite, with Scer\GAL4⁴⁷⁷

(Williams et al., 2006)

larval dorsal multidendritic neuron ddaC, with Scer\GAL4⁴⁷⁷

(Williams et al., 2006)

wing, with Scer\GAL4^ap-md544

(Shinoda et al., 2019)

Detailed Description

Statement

Reference

The expression of Dronc^{C318G.UAS.EGFP} under the control of Scer\GAL4^C10 does not affect the size of the adult wing, femur, tibia or tarsal segment 1.

Expression under the control of Scer\GAL4^ap-md544 leads to an opaque wing phenotype, indicative of apoptosis, but does not induce curly wings, which would indicate growth inhibition.

(Shinoda et al., 2019)

Overexpression of Nc^{ΔN.C-A.Scer\UAS} in the ddaC neurons under the control of Scer\GAL4⁴⁷⁷ results in a marked suppression of dendritic pruning. At 14 hours after pupal formation, the dendrites are detached from the soma but still largely cover the same 'footprint' as in third instar larvae. The morphology of detached branches varies: some show proximal swellings, whereas others are thinned and beaded along their entire length.

(Williams et al., 2006)

Expression of Nc^{C318G.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PF has little effect on the normal pattern of dying cells in the eye imaginal disc. No death during the pupal stage is seen in animals expressing Nc^{C318G.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PF and adult flies have normal eyes.

(Quinn et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^nub.PU/Dronc^{C318G.UAS.EGFP} is a suppressor | partially of visible | adult stage phenotype of RpS3^Plac92, yki^B5

(Wada et al., 2021)

Scer\GAL4^nub.PU/Dronc^{C318G.UAS.EGFP} is a suppressor | partially of decreased size | adult stage phenotype of RpS3^Plac92, yki^B5

(Wada et al., 2021)

Phenotype Manifest In

Enhancer of

Statement

Reference

Dronc^{C318G.UAS.EGFP}, Scer\GAL4^sca-P309 is an enhancer of macrochaeta phenotype of IKKε^RNAi.UAS, Scer\GAL4^sca-P309

(Koto et al., 2009)

Suppressor of

Statement

Reference

Scer\GAL4^nub.PU/Dronc^{C318G.UAS.EGFP} is a suppressor | partially of wing phenotype of RpS3^Plac92, yki^B5

(Wada et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Nc^{C318G.Scer\UAS.T:Avic\GFP-EGFP} in Scer\GAL4^sca-P309; ik2^{dsRNA.Scer\UAS} flies significantly enhances the short-bristle phenotype.

(Koto et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Dronc^{C318G.Scer\UAS.T:Avic\GFP-EGFP}

Dronc^{C318G.UAS.EGFP}

Nc^{C318G.Scer\UAS.T:Avic\GFP-EGFP}

Nc^{C318G.Scer\UAS.T:Avic\GFP-F64L.S65T}

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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