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General Information
Symbol
Dmel\Strica4
Species
D. melanogaster
Name
FlyBase ID
FBal0244459
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

Estimated boundaries of a 2.63 kb deletion resulting from the imprecise excision of P{XP}Stricad06491 that removes sequences from 355 bases upstream of the insertion to 70 bases from the end of Strica-RA.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the progenitor insertion, resulting in a 2.63kb deletion which removes genomic sequence from 355bp upstream of the original insertion site, the entire 527 amino acid dream coding region and all of the transcribed region of dream except the final 70bp.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Strica4 homozygotes do not exhibit a significant proportion of hyperplastic testes, as compared to controls.

The programmed cell death of bursCCAP neurons seen in the adult ventral nerve cord of wild type flies after eclosion is strongly suppressed in dream4 mutant homozygotes.

dream4 mutant males exhibit a similar amount of spermatogonial cyst death to wild type controls.

dream4 homozygotes exhibit wild-type apoptosis of vCrz neurons, with no viable vCrz neurons detected at 7 hours after puparium formation.

No abnormal mid-stage egg chambers are seen in the ovaries of homozygous or dream4/Df(2R)nap9 females.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

Strica4 has abnormal cell death phenotype, enhanceable by DroncI24

NOT Enhanced by
Statement
Reference

DroncI24, Strica4 has abnormal cell death phenotype, non-enhanceable by DreddB118

NOT suppressed by
Statement
Reference

DroncI24, Strica4 has abnormal cell death phenotype, non-suppressible by DreddB118

Enhancer of
Statement
Reference

Strica4 is an enhancer of abnormal cell death phenotype of DroncI24

NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

Strica4 has neuron phenotype, enhanceable by DroncI24

NOT Enhanced by
Statement
Reference

DroncI24, Strica4 has neuron phenotype, non-enhanceable by DreddB118

NOT suppressed by
Statement
Reference

DroncI24, Strica4 has neuron phenotype, non-suppressible by DreddB118

Enhancer of
Statement
Reference

Strica4 is an enhancer of neuron phenotype of DroncI24

Other
Additional Comments
Genetic Interactions
Statement
Reference

The programmed cell death of bursCCAP neurons seen in the adult ventral nerve cord of wild type flies after eclosion is strongly suppressed in dream4/DreddB118 mutants.

Nearly all vCrz neurons are present at 7 hours after puparium formation in dream4; NcI24 double mutants. Such an abnormal programmed cell death phenotype is significantly more severe than in either single mutant. No surviving vCrz neurons are found at 7 hours after puparium formation in dream4; NcI24/+ mutants, suggesting that heterozygosity of Nc is sufficient to compensate for the lack of dream function.

Triple mutants of DreddB118; dream4; NcI24 display results comparable to dream4; NcI24 double mutants, i.e. all vCrz neurons are still present having not undergone apoptosis by 7 hours after puparium formation.

dream4 ; NcI24/NcI29 IceΔ1 triple mutant larvae show high levels of TUNEL staining at -4 to -1 hours relative to puparium formation, suggesting that they are undergoing cell death.

Females containing dream4 ; NcI24 double homozygous germline clones are fertile, but their ovaries contain 31% of mid-stage egg chambers with missing follicle cells and large surviving nurse cells. There is also an increase in mid-stage egg chambers containing condensed nurse cell nuclei but lacking follicle cells. 16% of stage 14 egg chambers contain persisting nurse cell nuclei.

Females containing dream4 ; NcI29 double homozygous germline clones are fertile, but their ovaries contain 46% of mid-stage egg chambers with missing follicle cells and large surviving nurse cells. Many of these egg chambers contain excessive numbers of nurse cells. There is also an increase in mid-stage egg chambers containing condensed nurse cell nuclei but lacking follicle cells. 21% of stage 14 egg chambers contain persisting nurse cell nuclei.

No significant defects is seen in the egg chambers of females containing dream2/dream4 ; NcI29 double homozygous germline clones.

DreddB118 dream4 double mutant females do not show any defects during oogenesis.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (10)