FlyBase Allele Report: Dmel\TER94[E375Q.E575Q.UAS]

General Information

Symbol

Dmel\TER94^{E375Q.E575Q.UAS}

Species

D. melanogaster

Name

FlyBase ID

FBal0282575

Feature type

allele

Associated gene

Dmel\TER94

Associated Insertion(s)

Carried in Construct

P{UAS-TER94.E375Q.E575Q}

Also Known As

UAS-VCP QQ

Key Links

Transgenic product class

dominant_negative_variant

(Rumpf et al., 2011)

missense_variant

(Rumpf et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Rumpf et al., 2011)

missense_variant

(Rumpf et al., 2011)

Mutagen

in vitro construct

(Rumpf et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-TER94.E375Q.E575Q}

Cytology

Description

UAS regulatory sequences drive expression of an ATPase-deficient TER94 transgene (E375Q, E575Q) that acts as a dominant-negative.

(Rumpf et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

TER94

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of frontotemporal dementia

CEA

(Rumpf et al., 2014)

model of frontotemporal dementia and/or amyotrophic lateral sclerosis 6

CEC

(Rumpf et al., 2014)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of frontotemporal dementia

is ameliorated by Sox14^UAS.cRa

(Rumpf et al., 2014)

model of frontotemporal dementia and/or amyotrophic lateral sclerosis 6

is ameliorated by Sox14^UAS.cRa

(Rumpf et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^19-12

(Rumpf et al., 2011)

abnormal neuroanatomy | pupal stage, with Scer\GAL4^ppk.PG

(Rode et al., 2017)

abnormal neuroanatomy | RU486 conditional, with Scer\GAL4^{ppk.Switch.PR}

(Rumpf et al., 2011)

decreased cell death | pupal stage, with Scer\GAL4^19-12

(Rumpf et al., 2011)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of TER94^{E375Q.E575Q.Scer\UAS} under the control of Scer\GAL4^ppk.PG leads to strong dendrite pruning defects with approx. 80% of class IV dendrite arborizing neurons still having dendrites attached to the cell body at 18h after puparium formation.

(Rode et al., 2017)

Expression of TER94^{E375Q.E575Q.Scer\UAS} in class IV neurons (ddaC) under the control of Scer\GAL4^ppk.PG causes a reduction in dendrite arborization, while major branches stay intact. These defects in class IV neurons mostly affect higher-order branches containing filamentous actin, but not primary dendrite branches rich in microtubules.

Expression of TER94^{E375Q.E575Q.Scer\UAS} in class III neurons (ddaA and ddaF) under the control of Scer\GAL4^19-12 affects the length of class III dendrites, although spike appearance is normal. An additional copy of TER94^{E375Q.E575Q.Scer\UAS} increases the severity of the phenotype, with approximately 64% of all neurons displaying strong defects.

A high proportion of class IV neurons (ddaC) expressing TER94^{E375Q.E575Q.Scer\UAS} under the control of Scer\GAL4^ppk.PG retain long, attached dendrites (with 63% not severed).

Addition of 100υM RU486 to Scer\GAL4^{ppk.Switch.PR}/TER94^{E375Q.E575Q.Scer\UAS} larvae 24 hours before the onset of metamorphosis does not visibly change the morphology of the larval neurons; however, a high proportion of class IV neurons (ddaC) in these drug-treated animals retina their dendrites at 18 hours after pupal formation (defects occur in approximately 70% of neurons). indicating that the phenotypes observed during metamorphosis can be disassociated from the defects at the larval stage.

Class III neurons overexpressing TER94^{E375Q.E575Q.Scer\UAS} under the control of Scer\GAL4^19-12 do not undergo apoptosis and are still present by 18 hours after puparium formation. In these flies, the ddaF neuron is more prone to survive than ddaA.

(Rumpf et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^ppk.PG, TER94^{E375Q.E575Q.UAS} has abnormal neuroanatomy | pupal stage phenotype, non-enhanceable by Mfap1^GD5122, Scer\GAL4^ppk.PG

(Rode et al., 2017)

Scer\GAL4^ppk.PG, TER94^{E375Q.E575Q.UAS} has abnormal neuroanatomy | pupal stage phenotype, non-enhanceable by Mfap1[+]/Mfap1^DsRed

(Rode et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^19-12, TER94^{E375Q.E575Q.UAS} has decreased cell death | pupal stage phenotype, suppressible by Diap1⁴/th[+]

(Rumpf et al., 2011)

Scer\GAL4^ppk.PG, TER94^{E375Q.E575Q.UAS} has abnormal neuroanatomy phenotype, suppressible | partially by Diap1⁴/th[+]

(Rumpf et al., 2011)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^ppk.PG, TER94^{E375Q.E575Q.UAS} has larval multidendritic class IV neuron | pupal stage phenotype, non-enhanceable by Mfap1^GD5122, Scer\GAL4^ppk.PG

(Rode et al., 2017)

Scer\GAL4^ppk.PG, TER94^{E375Q.E575Q.UAS} has larval multidendritic class IV neuron | pupal stage phenotype, non-enhanceable by Mfap1[+]/Mfap1^DsRed

(Rode et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^ppk.PG, TER94^{E375Q.E575Q.UAS} has larval multidendritic class IV neuron phenotype, suppressible by Diap1⁴/th[+]

(Rumpf et al., 2011)

Scer\GAL4^ppk.PG, TER94^{E375Q.E575Q.UAS} has dendrite phenotype, suppressible by Diap1⁴/th[+]

(Rumpf et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The dendrite pruning defects in class IV dendrite arborizing neurons observed in pupae expressing TER94^{E375Q.E575Q.Scer\UAS} under the control of Scer\GAL4^ppk.PG is not significantly affected by co-expression of Mfap1^GD5122 or by combination with a single copy of Mfap1^DsRed.

(Rode et al., 2017)

A th⁴ heterozygous mutant background suppresses the class IV pruning defects seen upon expression of TER94^{E375Q.E575Q.Scer\UAS} under the control of Scer\GAL4^ppk.PG. Lowering th levels also sensitises cells to stress-induced apoptosis, and a high proportion of TER94^{E375Q.E575Q.Scer\UAS}-expressing neurons in a th⁴ heterozygous background undergo apoptosis during early metamorphosis (approximately 60% at 15 hours after pupal formation).

(Rumpf et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

TER94^{E375Q.E575Q.Scer\UAS}

TER94^{E375Q.E575Q.UAS}

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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