FlyBase Allele Report: Dmel\scny[GS13668]

General Information

Symbol

Dmel\scny^GS13668

Species

D. melanogaster

Name

FlyBase ID

FBal0240180

Feature type

allele

Associated gene

Dmel\scny

Associated Insertion(s)

P{GSV6}scny^GS13668

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GSV6}scny^GS13668

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{GSV6}

misexpression element

Regulatory region(s)

UASt

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:5,770,946..5,770,946

(Ribaya et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye | heat sensitive, with Scer\GAL4^GMR.PF

(Ribaya et al., 2009)

Detailed Description

Statement

Reference

At 18[o]C, flies expressing scny^GS13668 under the control of Scer\GAL4^GMR.PF have eyes that are normal in size and shape but which are slightly rough and show smoothness at the anterior portion of the eye. At 22[o]C, At 22[o]C, approximately 50% of the flies fail to eclose and die trapped in the pupal cases. The adults that survive have abnormal eye that are small and have a smooth or glossy phenotype. At 25[o]C, less than 1% of these animals eclose. Adults escapers have more severe eye defects than those grown at 22[o]C.

Most flies expressing scny^GS13668 under the control of Scer\GAL4^GMR.PF at 22 or 25[o]C have a scab of black tissue at the anterior region of the eye. This scab comes from a mass of tissue that is observed in pupae where it appears that cells within the eye are "leaking" out of the anterior portion of the developing eye, although it is unclear whether these cells are hemorrhaging from the eye or growing on top of the eye.

At 25[o]C, flies co-expressing scny^D.Scer\UAS and scny^GS13668 under the control of Scer\GAL4^GMR.PF have eyes that are larger and less rough than those expressing scny^GS13668 alone under the control of Scer\GAL4^GMR.PF.

(Ribaya et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, scny^GS13668 has lethal | heat sensitive phenotype, enhanceable by Diap1⁴/th[+]

(Ribaya et al., 2009)

Scer\GAL4^GMR.PF, scny^GS13668 has visible | heat sensitive phenotype, enhanceable by Diap1⁴/th[+]

(Ribaya et al., 2009)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, scny^GS13668 has lethal | heat sensitive phenotype, suppressible by Diap1^UAS.cHa, Scer\GAL4^GMR.PF

(Ribaya et al., 2009)

Scer\GAL4^GMR.PF, scny^GS13668 has visible | heat sensitive phenotype, suppressible by Diap1^UAS.cHa, Scer\GAL4^GMR.PF

(Ribaya et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, scny^GS13668 has eye | heat sensitive phenotype, enhanceable by Diap1⁴/th[+]

(Ribaya et al., 2009)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, scny^GS13668 has eye | heat sensitive phenotype, suppressible by Diap1^UAS.cHa, Scer\GAL4^GMR.PF

(Ribaya et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The semi-lethality seen at 22[o]C in animals expressing scny^GS13668 under the control of Scer\GAL4^GMR.PF is enhanced by th⁴/+, so that none of the double mutant animals eclose.

th⁴/+ enhances the severity of the rough eye phenotype caused by expression of scny^GS13668 under the control of Scer\GAL4^GMR.PF at 18[o]C.

Co-expression of th^Scer\UAS.cHa completely suppresses the lethality caused by expression of scny^GS13668 under the control of Scer\GAL4^GMR.PF at 25[o]C, and partially suppresses the mutant eye phenotype.

(Ribaya et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

204865

y¹ w^67c23; P{GSV6}scny^GS13668/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

et^GS13668

(Ribaya et al., 2009)

scny^GS13668

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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