Usp36, dUSP36, mule, ubiquitin-specific protease 36, emperor's thumb
protein deubiquitinase required in germline, epithelial, and intestinal stem cells - acts to repress differentiation genes - deubiquitylates histone H2B and functions in gene silencing - participates in a conserved pathway of chromatin regulation linking H2B ubiquitylation with H3K4me3 methylation
Gene model reviewed during 5.55
Gene model reviewed during 5.45
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.56
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\scny using the Feature Mapper tool.
Expressed cyclically in the adult fat body.
The expression of this gene along with several others is induced in salivary glands in pupae at the time of head eversion. This stage is characterized by an increase in the ecdysone titer as well as large amounts of cell death in this tissue.
GBrowse - Visual display of RNA-Seq signalsView Dmel\scny in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
scny negatively regulates the IMD pathway and suppresses fly immunity through its catalytic activity.
Usp36 encodes a deubiquitinating enzyme homologous to the human USP36 protein.
Usp36 mutant larvae are severely impaired in growth and die during the larval stages. They undergo the first larval moult more slowly than wild-type larvae and only a fraction of them undergo the second larval moult. The delayed first larval moult can be partially rescued by feeding ecdysone to the mutant larvae.
dsRNA made from templates generated with primers directed against mule that is transfected into S2 treated with Listeria monocytogenes reveals mule to be involved in Listeria monocytogenes intracellular growth, with increased intracellular growth observed in mule-treated cells.
Most loss of function mutants of mule have a lethal phenotype, however the authors original mule allele is viable, with only germline defects. mule mutant ovaries are very small and consist mostly of somatic tissue, however germline stem cells are still present.
Area matching Drosophila ESTs AA246386 and AA541060.
Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.
Overexpression of a long form of the gene inhibit apoptosis, whereas overexpression of the short form has a proapoptotic effect. The gene is thus named "emperor's thumb" for this ability to regulate both life (thumbs up) and death (thumbs down).