FB2025_05 , released December 11, 2025
Gene: Dmel\puf
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General Information
Symbol
Dmel\puf
Species
D. melanogaster
Name
puffyeye
Annotation Symbol
CG5794
Feature Type
protein_coding_gene
FlyBase ID
FBgn0039214
Gene Model Status
Current
Stock Availability
6 publicly available
Enzyme Name (EC)
ubiquitinyl hydrolase 1 (3.4.19.12)
Gene Summary
puffyeye (puf) encodes an essential deubiquitinating enzyme that acts as a ubiquitin-specific protease. It removes ubiquitin polypeptide chains from the products of Myc and CycE, thereby stabilizing these proteins and increasing their abundance to influence cell growth and proliferation. The product of puf binds to the product of ago as well as its substrates. It antagonizes ago function. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)
All Summaries
Also Known As

Usp34

Key Links
Genomic Location
Cytogenetic map
96A13-96A13
Sequence location
Recombination map
3-85
RefSeq locus
NT_033777 REGION:24635270..24650043
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (14 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables protein binding
inferred from physical interaction with UniProtKB:Q9VZF4
(Li et al., 2013)
inferred from physical interaction with UniProtKB:Q9W4S7
(Li et al., 2013)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables cysteine-type deubiquitinase activity
inferred from sequence or structural similarity with UniProtKB:Q70CQ2
(Gene Ontology Curators, 2002-)
inferred from electronic annotation with InterPro:IPR001394, InterPro:IPR018200
(InterPro Project Members, 2004-)
inferred from biological aspect of ancestor with PANTHER:PTN002541993
(GO Reference Genome Project, 2011-)
Biological Process (10 terms)
Terms Based on Experimental Evidence (9 terms)
CV Term
Evidence
References
involved_in negative regulation of antimicrobial peptide production
inferred from mutant phenotype
(Engel et al., 2014)
involved_in negative regulation of innate immune response
inferred from mutant phenotype
(Engel et al., 2014)
involved_in negative regulation of peptidoglycan recognition protein signaling pathway
inferred from mutant phenotype
(Engel et al., 2014)
involved_in negative regulation of Toll signaling pathway
inferred from mutant phenotype
(Engel et al., 2014)
involved_in positive regulation of cell growth
inferred from mutant phenotype
(Li et al., 2013)
inferred from genetic interaction with FLYBASE:Myc; FB:FBgn0262656
(Li et al., 2013)
involved_in positive regulation of double-strand break repair via homologous recombination
inferred from mutant phenotype
(Páhi et al., 2022)
involved_in post-translational protein modification
inferred from mutant phenotype
(Li et al., 2013)
involved_in regulation of protein stability
inferred from mutant phenotype
(Li et al., 2013)
involved_in wing disc development
inferred from mutant phenotype
(Li et al., 2013)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in protein deubiquitination
inferred from electronic annotation with InterPro:IPR001394
(InterPro Project Members, 2004-)
involved_in regulation of protein stability
inferred from biological aspect of ancestor with PANTHER:PTN002541993
(GO Reference Genome Project, 2011-)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in nucleus
inferred from direct assay
(Li et al., 2013)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
is_active_in cytosol
inferred from biological aspect of ancestor with PANTHER:PTN002541993
(GO Reference Genome Project, 2011-)
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN002541993
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
Belongs to the peptidase C19 family. (Q9VC56)
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
cysteine-type deubiquitinase activity
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal) (3.4.19.12)
Summaries
Gene Snapshot
puffyeye (puf) encodes an essential deubiquitinating enzyme that acts as a ubiquitin-specific protease. It removes ubiquitin polypeptide chains from the products of Myc and CycE, thereby stabilizing these proteins and increasing their abundance to influence cell growth and proliferation. The product of puf binds to the product of ago as well as its substrates. It antagonizes ago function. [Date last reviewed: 2019-03-14]
Gene Group (FlyBase)
USP DEUBIQUITINASES -
The Ubiquitin Specific Proteases (USP) is the largest sub-family of deubiquitinases. They are cysteine proteases and catalyze the removal of ubiquitin from ubiquitin chains and ubiquitinated proteins. USP deubiquitinases belong to MEROPS family C19. (Adapted from PMID:19626045).
Pathway (FlyBase)
NEGATIVE REGULATORS OF TOLL-NF-kappaB SIGNALING PATHWAY -
In Drosophila, the canonical Toll signaling pathway is initiated by the binding of a spatzle ligand to Toll (Tl) or a Toll-like receptor leading to the nuclear localization of the NF-κB (dl or Dif) transcription factor. Negative regulators of Toll-NF-κB Signaling lead to the increased cytosolic retention of the NF-κB proteins dl or Dif and/or repression of target gene transcription. (Adapted from FBrf0091014 and FBrf0223077).
NEGATIVE REGULATORS OF IMD SIGNALING PATHWAY -
Negative regulators of the immune deficiency (Imd) pathway result in the decreased activity of the NFκB-like transcription factor Rel in the nucleus. Negative regulators are important in preventing damage to the host from over-activation of the pathway; preventing inappropriate triggering or terminating the response. (Adapted from FBrf0224587 and FBrf0238555.)
Protein Function (UniProtKB)
Ubiquitin hydrolase that can remove conjugated ubiquitin from target proteins and polyubiquitin chains (PubMed:24173801). Essential for Myc-mediated cell growth and proliferation in developing eyes and wings (PubMed:24173801). In the wing and eye, the deubiquitinating activity acts as an antagonist to the SCF E3 ubiquitin-protein ligase member archipelago (ago) to regulate Myc and CycE stability and thus control cell growth and proliferation (PubMed:24173801). Also appears to regulate ago by modulating its induction by Myc (PubMed:24173801). May also promote cell apoptosis in the wing imaginal disk, acting in an apoptotic pathway that appears to be largely independent of Myc (PubMed:24173801). Required for preventing the activation of the immune deficiency (Imd) and Toll signaling cascades under unchallenged conditions (PubMed:25027767). Also appears to be involved in modulating the differential expression of certain antimicrobial peptides (AMP) in response to infection by either Gram-positive or Gram-negative bacteria (PubMed:25027767). Involved in the regulation of DNA damage repair pathways, including euchromatic site-specific double strand break (DSB) repair (PubMed:35393473).
(UniProt, Q9VC56)
Summary (Interactive Fly)

a ubiquitin-specific protease that regulates Myc levels and function - regulates both the ubiquitin ligase Archipelago and its cell cycle substrate Cyclin E

Gene Model and Products
Number of Transcripts
6
Number of Unique Polypeptides
6

Please see the JBrowse view of Dmel\puf for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VC56)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Stop-codon suppression (UAG) postulated; FBrf0216884.

Gene model reviewed during 5.44

Gene model reviewed during 5.49

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
puf-RD
FBtr0084668
NM_143018
13284
3912
puf-RE
FBtr0084667
NM_206564
8876
2832
puf-RF
FBtr0306172
NM_170158
13284
3912
puf-RG
FBtr0330308
NM_001275993
13232
3921
puf-RH
FBtr0330309
NM_001275994
13284
3930
puf-RI
FBtr0339135
NM_001300579
13311
3921
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
puf-PD
FBpp0084048
440.4
3912
6.16
Q9VC56
NP_651275
AAF56319
puf-PE
FBpp0084047
318.7
2832
5.75
Q7KS15
NP_996287
AAS65209
puf-PF
FBpp0297302
440.4
3912
6.16
Q8IMW2
NP_733022
AAN14009
puf-PG
FBpp0303340
441.3
3921
6.14
A0A0B4KHF3
NP_001262922
AGB96302
puf-PH
FBpp0303341
442.3
3930
6.14
A0A0B4KI06
NP_001262923
AGB96303
puf-PI
FBpp0308280
441.4
3921
6.14
A0A0B4LIK5
NP_001287508
AHN57507
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Interacts with Myc and ago.

(UniProt, Q9VC56)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\puf using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.21

Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 5 of adulthood | male -- day 7 of adulthood | male
spermatozoon
(Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in nucleus
inferred from direct assay
(Li et al., 2013)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\puf in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 5 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of puf
Transgenic constructs containing regulatory region of puf
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Df(3R)Exel7357
(Ryder, 2004.4.26)
Inferred from location ( 7 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal - all die before end of pupal stage | heat sensitive, with Scer\GAL4dpp.PU
lethal - all die during pupal stage, with Scer\GAL4Act.PU
partially lethal, with Scer\GAL4elav.PU
partially lethal, with Scer\GAL4sqh.PW
partially lethal - majority die
partially lethal - majority live, with Scer\GAL44XHand
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
male sterile
Other Phenotypes
Allele
abnormal immune response, with Scer\GAL4hs.PB
abnormal size, with Scer\GAL4GMR.PU
decreased cell size, with Scer\GAL4en.PU
increased cell death, with Scer\GAL4Bx-MS1096-KE
increased cell death, with Scer\GAL4en.PU
increased mortality during development | conditional, with Scer\GAL4da.PU
radiation sensitive | third instar larval stage, with Scer\GAL4da.PU
short lived
short lived, with Scer\GAL4elav.PU
short lived, with Scer\GAL4sqh.PW
some die during larval stage
some die during pupal stage
some die during pupal stage | heat sensitive, with Scer\GAL4dpp.PU
visible, with Scer\GAL4dpp.PU
Phenotype manifest in
Allele
eye, with Scer\GAL4GMR.PU
ommatidium, with Scer\GAL4GMR.PU
scutellar bristle, with Scer\GAL4dpp.PU
wing, with Scer\GAL4Bx-MS1096-KE
wing | posterior, with Scer\GAL4en.PU
wing vein, with Scer\GAL4en.PU
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (61)
Hsap\USP34
12 of 14
Yes
Yes
Hsap\USP24
3 of 14
No
No
Hsap\USP7
2 of 14
No
No
2  
Hsap\USP9X
2 of 14
No
No
Hsap\USP9Y
2 of 14
No
No
Hsap\USP18
2 of 14
No
No
Hsap\USP28
2 of 14
No
Yes
Hsap\USP47
2 of 14
No
No
Hsap\USP48
2 of 14
No
No
Hsap\USP2
1 of 14
No
No
Hsap\USP3
1 of 14
No
No
Hsap\USP8
1 of 14
No
No
1  
Hsap\USP10
1 of 14
No
No
Hsap\USP11
1 of 14
No
No
Hsap\USP12
1 of 14
No
No
Hsap\USP14
1 of 14
No
No
Hsap\USP15
1 of 14
No
No
Hsap\USP17L1
1 of 14
No
No
Hsap\USP17L2
1 of 14
No
No
Hsap\USP17L3
1 of 14
No
No
Hsap\USP17L4
1 of 14
No
No
Hsap\USP17L5
1 of 14
No
No
Hsap\USP17L6P
1 of 14
No
No
Hsap\USP17L7
1 of 14
No
No
Hsap\USP17L8
1 of 14
No
No
Hsap\USP17L10
1 of 14
No
No
Hsap\USP17L11
1 of 14
No
No
Hsap\USP17L12
1 of 14
No
No
Hsap\USP17L13
1 of 14
No
No
Hsap\USP17L15
1 of 14
No
No
Hsap\USP17L17
1 of 14
No
No
Hsap\USP17L18
1 of 14
No
No
Hsap\USP17L19
1 of 14
No
No
Hsap\USP17L20
1 of 14
No
No
Hsap\USP17L21
1 of 14
No
No
Hsap\USP17L22
1 of 14
No
No
Hsap\USP17L24
1 of 14
No
No
Hsap\USP17L25
1 of 14
No
No
Hsap\USP17L26
1 of 14
No
No
Hsap\USP17L27
1 of 14
No
No
Hsap\USP17L28
1 of 14
No
No
Hsap\USP17L29
1 of 14
No
No
Hsap\USP17L30
1 of 14
No
No
Hsap\USP20
1 of 14
No
No
Hsap\USP21
1 of 14
No
No
Hsap\USP22
1 of 14
No
No
Hsap\USP25
1 of 14
No
No
Hsap\USP27X
1 of 14
No
No
Hsap\USP30
1 of 14
No
No
3  
Hsap\USP33
1 of 14
No
No
Hsap\USP35
1 of 14
No
No
Hsap\USP36
1 of 14
No
No
Hsap\USP38
1 of 14
No
No
Hsap\USP41
1 of 14
No
No
Hsap\USP42
1 of 14
No
No
Hsap\USP43
1 of 14
No
No
Hsap\USP44
1 of 14
No
No
Hsap\USP46
1 of 14
No
No
1  
Hsap\USP49
1 of 14
No
No
Hsap\USP50
1 of 14
No
No
Hsap\USP51
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (26)
Rnor\Usp34
11 of 14
Yes
Yes
Rnor\Usp7
2 of 14
No
No
Rnor\Usp9x
2 of 14
No
No
Rnor\Usp18
2 of 14
No
No
Rnor\Usp24
2 of 14
No
No
Rnor\Usp28
2 of 14
No
Yes
Rnor\Usp47
2 of 14
No
No
Rnor\Usp48
2 of 14
No
No
Rnor\Usp3
1 of 14
No
No
Rnor\Usp8
1 of 14
No
No
Rnor\Usp9y
1 of 14
No
No
Rnor\Usp12
1 of 14
No
No
Rnor\Usp14
1 of 14
No
No
Rnor\Usp20
1 of 14
No
No
Rnor\Usp21
1 of 14
No
No
Rnor\Usp22
1 of 14
No
No
Rnor\Usp25
1 of 14
No
No
Rnor\Usp27x
1 of 14
No
No
Rnor\Usp33
1 of 14
No
No
Rnor\Usp35
1 of 14
No
No
Rnor\Usp36
1 of 14
No
No
Rnor\Usp38
1 of 14
No
No
Rnor\Usp40
1 of 14
No
No
Rnor\Usp46
1 of 14
No
No
Rnor\Usp50
1 of 14
No
No
Rnor\Usp51
1 of 14
No
No
Mus musculus (laboratory mouse) (24)
Mmus\Usp34
11 of 14
Yes
Yes
Mmus\Usp24
3 of 14
No
No
Mmus\Usp7
2 of 14
No
No
Mmus\Usp9x
2 of 14
No
No
4  
Mmus\Usp9y
2 of 14
No
No
Mmus\Usp18
2 of 14
No
No
Mmus\Usp28
2 of 14
No
Yes
Mmus\Usp47
2 of 14
No
No
Mmus\Usp48
2 of 14
No
Yes
Mmus\Usp3
1 of 14
No
No
Mmus\Usp12
1 of 14
No
No
Mmus\Usp14
1 of 14
No
No
Mmus\Usp17la
1 of 14
No
No
Mmus\Usp20
1 of 14
No
No
Mmus\Usp22
1 of 14
No
No
Mmus\Usp25
1 of 14
No
No
Mmus\Usp27x
1 of 14
No
No
Mmus\Usp33
1 of 14
No
No
Mmus\Usp35
1 of 14
No
No
Mmus\Usp36
1 of 14
No
No
Mmus\Usp38
1 of 14
No
No
Mmus\Usp40
1 of 14
No
No
Mmus\Usp46
1 of 14
No
No
Mmus\Usp50
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (24)
Xtro\usp34
8 of 13
Yes
Yes
Xtro\usp24
2 of 13
No
No
Xtro\acaca
1 of 13
No
No
Xtro\amacr
1 of 13
No
No
Xtro\btd
1 of 13
No
No
Xtro\LOC116410989
1 of 13
No
No
Xtro\usp9x
1 of 13
No
No
Xtro\usp10
1 of 13
No
No
Xtro\usp12b
1 of 13
No
No
Xtro\usp12
1 of 13
No
No
Xtro\usp14
1 of 13
No
No
Xtro\usp15
1 of 13
No
No
Xtro\usp20
1 of 13
No
No
Xtro\usp21
1 of 13
No
No
Xtro\usp22
1 of 13
No
No
Xtro\usp28
1 of 13
No
Yes
Xtro\usp30
1 of 13
No
No
Xtro\usp33
1 of 13
No
No
Xtro\usp38
1 of 13
No
No
Xtro\usp44
1 of 13
No
Yes
Xtro\usp47
1 of 13
No
No
Xtro\usp48
1 of 13
No
No
Xtro\usp49
1 of 13
No
No
Xtro\yrdc
1 of 13
No
No
Danio rerio (Zebrafish) (23)
Drer\usp34
11 of 14
Yes
Yes
Drer\usp7
2 of 14
No
No
Drer\usp18
2 of 14
No
Yes
Drer\usp24
2 of 14
No
No
Drer\usp40
2 of 14
No
No
Drer\si:ch211-212k18.13
1 of 14
No
Yes
Drer\usp2b
1 of 14
No
No
Drer\usp3
1 of 14
No
No
Drer\usp9
1 of 14
No
No
Drer\usp12a
1 of 14
No
No
Drer\usp12b
1 of 14
No
No
Drer\usp14
1 of 14
No
No
Drer\usp22
1 of 14
No
No
Drer\usp25
1 of 14
No
Yes
Drer\usp28
1 of 14
No
Yes
Drer\usp33
1 of 14
No
No
Drer\usp36
1 of 14
No
No
Drer\usp38
1 of 14
No
No
Drer\usp44
1 of 14
No
No
Drer\usp46
1 of 14
No
No
Drer\usp47
1 of 14
No
No
Drer\usp48
1 of 14
No
No
Drer\usp49
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (18)
Cele\T05H10.1
2 of 14
Yes
No
Cele\T24B8.7
2 of 14
Yes
No
Cele\usp-48
2 of 14
Yes
No
Cele\C34F6.9
1 of 14
No
Yes
Cele\cyk-3
1 of 14
No
No
Cele\F07A11.4
1 of 14
No
No
Cele\H12I13.1
1 of 14
No
No
Cele\K02C4.3
1 of 14
No
Yes
Cele\K08B4.5
1 of 14
No
Yes
Cele\math-33
1 of 14
No
No
Cele\T22F3.2
1 of 14
No
No
Cele\usp-3
1 of 14
No
No
Cele\usp-14
1 of 14
No
No
Cele\usp-33
1 of 14
No
No
Cele\usp-39
1 of 14
No
No
Cele\usp-46
1 of 14
No
No
Cele\usp-50
1 of 14
No
No
Cele\Y67D2.2
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (16)
Agam\AgaP_AGAP003643
11 of 12
Yes
Yes
Agam\AgaP_AGAP007106
2 of 12
No
No
Agam\AgaP_AGAP008530
2 of 12
No
No
Agam\AgaP_AGAP010088
2 of 12
No
No
Agam\AgaP_AGAP000269
1 of 12
No
No
Agam\AgaP_AGAP001052
1 of 12
No
No
Agam\AgaP_AGAP002900
1 of 12
No
No
Agam\AgaP_AGAP003821
1 of 12
No
No
Agam\AgaP_AGAP006652
1 of 12
No
No
Agam\AgaP_AGAP007521
1 of 12
No
No
Agam\AgaP_AGAP007600
1 of 12
No
No
Agam\AgaP_AGAP008805
1 of 12
No
No
Agam\AgaP_AGAP009348
1 of 12
No
No
Agam\AgaP_AGAP010080
1 of 12
No
No
Agam\AgaP_AGAP012119
1 of 12
No
No
Agam\AgaP_AGAP012139
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (22)
Atha\UBP26
3 of 13
Yes
Yes
Atha\UBP12
2 of 13
No
No
Atha\UBP13
2 of 13
No
No
Atha\UBP1
1 of 13
No
No
Atha\UBP2
1 of 13
No
No
Atha\UBP3
1 of 13
No
No
Atha\UBP4
1 of 13
No
No
Atha\UBP6
1 of 13
No
No
Atha\UBP7
1 of 13
No
No
Atha\UBP8
1 of 13
No
No
Atha\UBP15
1 of 13
No
No
Atha\UBP16
1 of 13
No
No
Atha\UBP17
1 of 13
No
No
Atha\UBP18
1 of 13
No
No
Atha\UBP19
1 of 13
No
No
Atha\UBP20
1 of 13
No
No
Atha\UBP21
1 of 13
No
No
Atha\UBP22
1 of 13
No
No
Atha\UBP23
1 of 13
No
No
Atha\UBP24
1 of 13
No
No
Atha\UBP25
1 of 13
No
No
Atha\UBP27
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (10)
Scer\UBP2
2 of 13
Yes
Yes
3  
Scer\UBP15
2 of 13
Yes
No
Scer\DOA4
1 of 13
No
No
1  
Scer\UBP3
1 of 13
No
No
Scer\UBP5
1 of 13
No
No
Scer\UBP6
1 of 13
No
No
Scer\UBP8
1 of 13
No
No
Scer\UBP9
1 of 13
No
No
Scer\UBP10
1 of 13
No
No
Scer\UBP13
1 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (4)
Spom\ubp5
2 of 12
Yes
No
Spom\ubp15
2 of 12
Yes
No
Spom\ubp2
1 of 12
No
Yes
Spom\ubp6
1 of 12
No
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:puf. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (18)
faf
4 of 13
Usp7
3 of 13
Usp47
3 of 13
DUBAI
2 of 13
scny
2 of 13
Usp5
2 of 13
Usp10
2 of 13
Usp12-46
2 of 13
Usp30
2 of 13
not
1 of 13
Usp1
1 of 13
Usp2
1 of 13
Usp8
1 of 13
Usp14
1 of 13
Usp15-31
1 of 13
Usp16-45
1 of 13
Usp20-33
1 of 13
Usp32
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    USP34; ubiquitin specific peptidase 34
    12 of 14
    615295
        USP24; ubiquitin specific peptidase 24
        3 of 14
        610569
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            Summary of Genetic Interactions
            Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            puf
            enhanceable
            ago
            (Li et al., 2013)
            puf
            suppressible
            Diap1
            (Li et al., 2013)
            puf
            suppressible
            Myc
            (Li et al., 2013)
            puf
            enhanceable
            Myc
            (Li et al., 2013)
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            ago|Myc
            enhanceable
            puf
            (Li et al., 2013)
            bw
            suppressible
            puf
            (Schneiderman et al., 2010)
            Myc
            suppressible
            puf
            (Li et al., 2013)
            Myc
            enhanceable
            puf
            (Li et al., 2013)
            Myc|ago
            suppressible
            puf
            (Li et al., 2013)
            External Data
            Subunit Structure (UniProtKB)
            Interacts with Myc and ago.
            (UniProt, Q9VC56 )
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            FlyBase
            External Links
            External Data
            Linkouts
            Class of Gene
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            3R
            Recombination map
            3-85
            Cytogenetic map
            96A13-96A13
            Sequence location
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            96A13-96A13
            Limits computationally determined from genome sequence between P{PZ}crb07207&P{PZ}BRWD305842 and P{EP}CycB3EP3127
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            Experimentally Determined Recombination Data
            Location

            3-85

            (FlyBase, 2016)

            3-84.1

            (Comeron et al., 2012)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (6)
            Genomic Clones (25)
            cDNA Clones (16)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
              Drosophila Genomics Resource Center cDNA clones

              For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

              cDNA Clones, End Sequenced (ESTs)
              BDGP DGC clones
              Other clones
              RNAi and Array Information
              Linkouts
              DRSC - Results frm RNAi screens
              Antibody Information
              Laboratory Generated Antibodies
               

              polyclonal

              (Li et al., 2013)
              Commercially Available Antibodies
               
              Cell Line Information
              Publicly Available Cell Lines
               
                Other Stable Cell Lines
                 
                  Other Comments
                  Relationship to Other Genes
                  Source for database merge of

                  Source for merge of: CG5794 NEST:bs19d10

                  (Rana, 2004.7.8)

                  Source for merge of: CG5794 BcDNA:AT28817

                  (FlyBase, 1996-)
                  Additional comments

                  Source for merge of CG5794 BcDNA:AT28817 was a shared cDNA ( date:030728 ).

                  (FlyBase, 1996-)
                  Nomenclature History
                  Source for database identify of

                  Source for identity of: puf CG5794

                  (Li et al., 2013)
                  Nomenclature comments
                  Etymology

                  Named 'puffyeye' to reflect its role in the 'dm'-induced rough eye phenotype.

                  (Li et al., 2013)
                  Synonyms and Secondary IDs (9)
                  Datasets (0)
                  Study focus (0)
                  Experimental Role
                  Project
                  Project Type
                  Title
                  Study result (0)
                  Result
                  Result Type
                  Title
                  External Crossreferences and Linkouts ( 64 )
                  Sequence Crossreferences
                  NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                  GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                  GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                  RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                  UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                  UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                  UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                  Other crossreferences
                  AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                  DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                  EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                  FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                  FlyMine - An integrated database for Drosophila genomics
                  InterPro - A database of protein families, domains and functional sites
                  KEGG Genes - Molecular building blocks of life in the genomic space.
                  MARRVEL_MODEL - MARRVEL (model organism gene)
                  Linkouts
                  BioGRID - A database of protein and genetic interactions.
                  Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                  DroID - A comprehensive database of gene and protein interactions.
                  DRSC - Results frm RNAi screens
                  Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                  FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                  FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                  Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                  Flygut - An atlas of the Drosophila adult midgut
                  FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                  iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                  Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                  MIST (genetic) - An integrated Molecular Interaction Database
                  MIST (protein-protein) - An integrated Molecular Interaction Database
                  References (60)