FlyBase Allele Report: Scer\GAL4[4XHand]

General Information

Symbol

Scer\GAL4^4XHand

Species

S. cerevisiae

Name

FlyBase ID

FBal0343913

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{4XHand-GAL4}

Also Known As

4XHand-Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Zhu et al., 2017)

Progenitor genotype

Carried in construct

P{4XHand-GAL4}

Cytology

Description

4 tandem insertions of the original Hand cardiac-specific enhancer (see FBrf0187463) drive expression of GAL4.

(Zhu et al., 2017)

Allele components

Component

Use(s)

Regulatory region(s)

Hand

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

cardial cell

(Zhu et al., 2017)

adult pericardial cell

(Zhu et al., 2017)

Additional Information

Statement

Reference

ScerGAL4^4XHand drives significantly greater GAL4 production than constructs using a single copy of the Hand enhancer.

(Zhu et al., 2017)

Marker for

Reflects expression of

Hand

(Zhu et al., 2017)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | adult stage, with kis^RNAi.UAS.cUa

(Zhu et al., 2017)

decreased cell number | adult stage, with trx^RNAi.UAS.cUa

(Zhu et al., 2017)

decreased cell number | adult stage, with Ubc6^RNAi.UAS.cUa

(Zhu et al., 2017)

lethal, with ssh^HMS01862

(Zhu et al., 2017)

lethal, with wds^RNAi.UAS.cUa

(Zhu et al., 2017)

partially lethal, with Naa15-16^HMS01400

(Zhu et al., 2025)

partially lethal, with Ptx1^RNAi.UAS.cUa

(Zhu et al., 2017)

partially lethal, with san^GL00592

(Zhu et al., 2025)

partially lethal, with vnc^HMC03479

(Zhu et al., 2025)

partially lethal - majority die, with kis^RNAi.UAS.cUa

(Zhu et al., 2017)

partially lethal - majority die, with mtt^HMS02793

(Zhu et al., 2017)

partially lethal - majority die, with NAT1^HMS00348

(Zhu et al., 2017)

partially lethal - majority die, with Pym^GL00515

(Zhu et al., 2017)

partially lethal - majority die, with rump^HMS02501

(Zhu et al., 2017)

partially lethal - majority die, with Spt5^RNAi.UAS.cUa

(Zhu et al., 2017)

partially lethal - majority die, with Ubc6^RNAi.UAS.cUa

(Zhu et al., 2017)

partially lethal - majority live, with BicD^GL00325

(Zhu et al., 2017)

partially lethal - majority live, with Bre1^RNAi.UAS.cUa

(Zhu et al., 2017)

partially lethal - majority live, with Cul3^RNAi.UAS.cUa

(Zhu et al., 2017)

partially lethal - majority live, with Hmt4-20^RNAi.UAS.cUa

(Zhu et al., 2017)

partially lethal - majority live, with ptc^JF03223

(Zhu et al., 2017)

partially lethal - majority live, with puf^RNAi.UAS.cUa

(Zhu et al., 2017)

partially lethal - majority live, with scny^HMS02044

(Zhu et al., 2017)

partially lethal - majority live, with Sos^RNAi.UAS.cUa

(Zhu et al., 2017)

partially lethal - majority live, with trx^RNAi.UAS.cUa

(Zhu et al., 2017)

partially lethal - majority live, with twf^HMC04627

(Zhu et al., 2017)

short lived, with kis^RNAi.UAS.cUa

(Zhu et al., 2017)

short lived, with Naa15-16^HMS01400

(Zhu et al., 2025)

short lived, with san^GL00592

(Zhu et al., 2025)

short lived, with trx^RNAi.UAS.cUa

(Zhu et al., 2017)

short lived, with Ubc6^RNAi.UAS.cUa

(Zhu et al., 2017)

short lived, with vnc^HMC03479

(Zhu et al., 2025)

short lived, with wds^RNAi.UAS.cUa

(Zhu et al., 2017)

Phenotype Manifest In

adult heart, with Act57B^M306L.UAS

(Viswanathan et al., 2020)

adult heart, with kis^RNAi.UAS.cUa

(Zhu et al., 2017)

adult heart, with Naa15-16^HMS01400

(Zhu et al., 2025)

adult heart, with san^GL00592

(Zhu et al., 2025)

adult heart, with trx^RNAi.UAS.cUa

(Zhu et al., 2017)

adult heart, with Ubc6^RNAi.UAS.cUa

(Zhu et al., 2017)

adult heart, with vnc^HMC03479

(Zhu et al., 2025)

adult heart, with wds^RNAi.UAS.cUa

(Zhu et al., 2017)

cardiac myofibril, with Naa15-16^HMS01400

(Zhu et al., 2025)

cardiac myofibril, with san^GL00592

(Zhu et al., 2025)

cardiac myofibril, with vnc^HMC03479

(Zhu et al., 2025)

cardiac myofibril | adult stage, with Bre1^RNAi.UAS.cUa

(Zhu et al., 2017)

cardiac myofibril | adult stage, with kis^RNAi.UAS.cUa

(Zhu et al., 2017)

cardiac myofibril | adult stage, with scny^HMS02044

(Zhu et al., 2017)

cardiac myofibril | adult stage, with trx^RNAi.UAS.cUa

(Zhu et al., 2017)

cardiac myofibril | adult stage, with Ubc6^RNAi.UAS.cUa

(Zhu et al., 2017)

cardiac myofibril | larval stage, with kis^RNAi.UAS.cUa

(Zhu et al., 2017)

cardiac myofibril | larval stage, with trx^RNAi.UAS.cUa

(Zhu et al., 2017)

cardiac myofibril | larval stage, with Ubc6^RNAi.UAS.cUa

(Zhu et al., 2017)

cardiac myofibril | larval stage, with wds^RNAi.UAS.cUa

(Zhu et al., 2017)

dorsal vessel wall cell | adult stage, with kis^RNAi.UAS.cUa

(Zhu et al., 2017)

dorsal vessel wall cell | adult stage, with trx^RNAi.UAS.cUa

(Zhu et al., 2017)

dorsal vessel wall cell | adult stage, with Ubc6^RNAi.UAS.cUa

(Zhu et al., 2017)

embryonic/larval heart | larval stage, with kis^RNAi.UAS.cUa

(Zhu et al., 2017)

embryonic/larval heart | larval stage, with trx^RNAi.UAS.cUa

(Zhu et al., 2017)

embryonic/larval heart | larval stage, with Ubc6^RNAi.UAS.cUa

(Zhu et al., 2017)

embryonic/larval heart | larval stage, with wds^RNAi.UAS.cUa

(Zhu et al., 2017)

extracellular matrix | adult stage, with kis^RNAi.UAS.cUa

(Zhu et al., 2017)

extracellular matrix | adult stage, with trx^RNAi.UAS.cUa

(Zhu et al., 2017)

extracellular matrix | adult stage, with Ubc6^RNAi.UAS.cUa

(Zhu et al., 2017)

extracellular matrix | larval stage, with kis^RNAi.UAS.cUa

(Zhu et al., 2017)

extracellular matrix | larval stage, with trx^RNAi.UAS.cUa

(Zhu et al., 2017)

extracellular matrix | larval stage, with Ubc6^RNAi.UAS.cUa

(Zhu et al., 2017)

extracellular matrix | larval stage, with wds^RNAi.UAS.cUa

(Zhu et al., 2017)

filamentous actin | adult stage, with san^GL00592

(Zhu et al., 2025)

filamentous actin | adult stage, with vnc^HMC03479

(Zhu et al., 2025)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^4XHand, wds^RNAi.UAS.cUa has lethal phenotype, suppressible | partially by Hsap\WDR5^WT.UAS, Scer\GAL4^4XHand

(Zhu et al., 2017)

Scer\GAL4^4XHand, wds^RNAi.UAS.cUa has lethal phenotype, suppressible | partially by Hsap\WDR5^K7Q.UAS, Scer\GAL4^4XHand

(Zhu et al., 2017)

Suppressor of

Statement

Reference

Hsap\WDR5^WT.UAS, Scer\GAL4^4XHand is a suppressor | partially of lethal phenotype of Scer\GAL4^4XHand, wds^RNAi.UAS.cUa

(Zhu et al., 2017)

Hsap\WDR5^K7Q.UAS, Scer\GAL4^4XHand is a suppressor | partially of lethal phenotype of Scer\GAL4^4XHand, wds^RNAi.UAS.cUa

(Zhu et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Naa15-16^HMS01400, Scer\GAL4^4XHand has adult heart phenotype, suppressible by Hsap\NAA16^UAS.cZa, Scer\GAL4^4XHand

(Zhu et al., 2025)

Naa15-16^HMS01400, Scer\GAL4^4XHand has cardiac myofibril phenotype, suppressible by Hsap\NAA16^UAS.cZa, Scer\GAL4^4XHand

(Zhu et al., 2025)

Scer\GAL4^4XHand, wds^RNAi.UAS.cUa has cardiac myofibril | larval stage phenotype, suppressible | partially by Hsap\WDR5^K7Q.UAS, Scer\GAL4^4XHand

(Zhu et al., 2017)

Scer\GAL4^4XHand, wds^RNAi.UAS.cUa has extracellular matrix | larval stage phenotype, suppressible by Hsap\WDR5^WT.UAS, Scer\GAL4^4XHand

(Zhu et al., 2017)

Scer\GAL4^4XHand, wds^RNAi.UAS.cUa has extracellular matrix | larval stage phenotype, suppressible | partially by Hsap\WDR5^K7Q.UAS, Scer\GAL4^4XHand

(Zhu et al., 2017)

Scer\GAL4^4XHand, wds^RNAi.UAS.cUa has cardiac myofibril | larval stage phenotype, suppressible by Hsap\WDR5^WT.UAS, Scer\GAL4^4XHand

(Zhu et al., 2017)

NOT suppressed by

Statement

Reference

Naa15-16^HMS01400, Scer\GAL4^4XHand has adult heart phenotype, non-suppressible by Hsap\NAA16^R70C.UAS, Scer\GAL4^4XHand

(Zhu et al., 2025)

Naa15-16^HMS01400, Scer\GAL4^4XHand has cardiac myofibril phenotype, non-suppressible by Hsap\NAA16^R70C.UAS, Scer\GAL4^4XHand

(Zhu et al., 2025)

Suppressor of

Statement

Reference

Hsap\NAA16^UAS.cZa, Scer\GAL4^4XHand is a suppressor of adult heart phenotype of Naa15-16^HMS01400, Scer\GAL4^4XHand

(Zhu et al., 2025)

Hsap\NAA16^UAS.cZa, Scer\GAL4^4XHand is a suppressor of cardiac myofibril phenotype of Naa15-16^HMS01400, Scer\GAL4^4XHand

(Zhu et al., 2025)

Hsap\WDR5^K7Q.UAS, Scer\GAL4^4XHand is a suppressor | partially of cardiac myofibril | larval stage phenotype of Scer\GAL4^4XHand, wds^RNAi.UAS.cUa

(Zhu et al., 2017)

Hsap\WDR5^WT.UAS, Scer\GAL4^4XHand is a suppressor of extracellular matrix | larval stage phenotype of Scer\GAL4^4XHand, wds^RNAi.UAS.cUa

(Zhu et al., 2017)

Hsap\WDR5^K7Q.UAS, Scer\GAL4^4XHand is a suppressor | partially of extracellular matrix | larval stage phenotype of Scer\GAL4^4XHand, wds^RNAi.UAS.cUa

(Zhu et al., 2017)

Hsap\WDR5^WT.UAS, Scer\GAL4^4XHand is a suppressor of cardiac myofibril | larval stage phenotype of Scer\GAL4^4XHand, wds^RNAi.UAS.cUa

(Zhu et al., 2017)

NOT Suppressor of

Statement

Reference

Hsap\NAA16^R70C.UAS, Scer\GAL4^4XHand is a non-suppressor of adult heart phenotype of Naa15-16^HMS01400, Scer\GAL4^4XHand

(Zhu et al., 2025)

Hsap\NAA16^R70C.UAS, Scer\GAL4^4XHand is a non-suppressor of cardiac myofibril phenotype of Naa15-16^HMS01400, Scer\GAL4^4XHand

(Zhu et al., 2025)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

606647

w^*; P{4XHand-GAL4}2, P{UAS-sfGFP-TurboID-ER}attP40

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^4XHand

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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