FlyBase Allele Report: Dmel\scny[f01742]

General Information

Symbol

Dmel\scny^f01742

Species

D. melanogaster

Name

FlyBase ID

FBal0178774

Feature type

allele

Associated gene

Dmel\scny

Associated Insertion(s)

PBac{WH}scny^f01742

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

piggyBac activity

Nature of the Allele

Allele class

Mutagen

piggyBac activity

(Gene Disruption Project members and Exelixis, 2005)

Progenitor genotype

Associated Insertion(s)

PBac{WH}scny^f01742

Cytology

Description

Allele components

Component

Use(s)

Inserted element

PBac{WH}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:5,771,585..5,771,585 [+]

(Ribaya et al., 2009, Gene Disruption Project members and Exelixis, 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile (with scny⁰²³³¹)

(Buszczak et al., 2009)

lethal (with Df(3L)ZN47)

(Buszczak et al., 2009)

partially lethal - majority die (with scny⁰²³³¹)

(Buszczak et al., 2009)

partially lethal - majority die | recessive

(Buszczak et al., 2009)

sterile

(Ribaya et al., 2009)

viable

(Ribaya et al., 2009)

viable (with scny^d06513)

(Buszczak et al., 2009)

viable (with scny^e00340)

(Buszczak et al., 2009)

Phenotype Manifest In

female germline stem cell | somatic clone

(Buszczak et al., 2009)

follicle stem cell | somatic clone

(Buszczak et al., 2009)

intestinal stem cell (with scny⁰²³³¹)

(Buszczak et al., 2009)

intestinal stem cell & adult midgut

(Buszczak et al., 2009)

Detailed Description

Statement

Reference

The half life of follicle stem cells in Usp36^f01742 homozygous clones significantly reduced compared to wild-type. But mutant follicle cells develop normally.

In Usp36^f01742/Usp36⁰²³³¹ adults, intestinal stem cells are present at near normal numbers at eclosion but are rapidly lost so that few are left by 7 days post eclosion. This phenotype is partially suppressed by the addition of 8mM DAPT (a gamma-secretase inhibitor) to the food. Unlike wild-type adults, mutants treated with DAPT do not form tumors.

(Buszczak et al., 2009)

The half life of female germline stem cells in Usp36^f01742 somatic clones is sharply reduced compared to wild-type. Marker expression studies indicate that germ cells in the female germline stem cell begin to differentiate prematurely.

(Buszczak et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Usp36^f01742 dominantly suppresses position effect variegation in the gastric secae due to Tp(3;Y)BL2.

Usp36^f01742 ; bam^Δ86 double homozygotes have increased numbers of female germline stem cells compared to wild-type, as bam^Δ86 homozygotes do.

The midgut of 7 day old Usp36^f01742 homozygous adults has greatly reduced numbers of intestinal stem cells.

(Buszczak et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

85194

w¹¹¹⁸; PBac{WH}scny^f01742

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Usp36^f01742

et^f01742

(Ribaya et al., 2009)

mule^f01742

scny^f01742

(Buszczak et al., 2009, Buszczak et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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