A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
The presence of Df(3L)BSC884 was verified using the PCR methods and primers described in FBrf0175003, with the substitution of the primer 5’-GCTTCTAAACGCTTACGCATAAACGATG-3’ for the RB3’ plus or RB3’ minus primer in the Hybrid PCR protocol in the Supplementary Methods.
The breakpoints of Df(3L)BSC884 predicted from the Release 5 genomic coordinates of the progenitor P{XP}Eaf6d06605 and PBac{RB}CG32418e03237 transposable element insertion sites are 3R:5601375 ;5770185 and the cytological breakpoints predicted from these coordinates are 64D6;64E7.