FB2021_02, released April 13, 2021

Allele: Dmel\fitUAS.Tag:HA

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General Information
Symbol
Dmel\fitUAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0325461
Feature type
allele
Associated gene
Dmel\fit
Associated Insertion(s)
Carried in Construct
P{UAS-fit.HA}
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - coding region fusion
(Sun et al., 2017)
in vitro construct - regulatory fusion
(Sun et al., 2017)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{UAS-fit.HA}
      Cytology
      Nature of the lesion
      Statement
      Reference

      UASt regulatory sequences drive expression of full length fit cDNA, tagged with Tag:HA.

      (Sun et al., 2017)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UASt
      Encoded product / tool
      fit
      Tagged with
      Tag:HA
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Adult (unstarved) females expressing fitScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4fit.PS show significant decrease in the preference for tryptone-supplemented (source of amino-acids) food both after sucrose pre-feeding or without any pre-feeding (this is accompanied by corresponding increase in preference for sucrose-only food in the latter case). Adult males also show reduced preference for tryptone food upon sugar pre-feeding but no aberrations in food choices are observed when no pre-feeding is administered. Both sexes display normal food choices after tryptone pre-feeding. The decrease in tryptone food preference after a sucrose pre-feeding is also observed when the fitScer\UAS.T:Ivir\HA1 transgene is driven by Scer\GAL4elav-C155.

      Pre-starved adult flies expressing fitScer\UAS.T:Ivir\HA1 under the control of any of the following drivers Scer\GAL4fit.PS, Scer\GAL4Lsp2.3.1, Scer\GAL4ppl.PP, Scer\GAL4elav-C155, Scer\GAL4elav.Switch.PO (using RU486 to limit the expression to adulthood) or Scer\GAL4Ilp2.PU display significant decrease in food consumption upon re-feeding compared to controls.

      (Sun et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      NOT Suppressor of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The decrease in preference for tryptone-supplemented food over a sucrose only food characteristic for unstarved adult flies expressing fitScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4elav-C155 in a two-way choice assay is suppressed by co-expression of InRK1409A.Scer\UAS.

      The overfeeding behavior observed in pre-starved adult flies expressing InRK1409A.Scer\UAS under the control of Scer\GAL4elav-C155 upon re-feeding is not suppressed by co-expression of fitScer\UAS.T:Ivir\HA1.

      (Sun et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      fitUAS.Tag:HA/Scer\GAL4fit.PS rescues fit81

      (Sun et al., 2017)
      Comments

      Expression of fitScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4fit.PS restores the disrupted suppressive effect of tryptone pre-feeding on feeding (on standard food) in fit81 adult females.

      (Sun et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      fitScer\UAS.T:Ivir\HA1
      fitUAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (1)