Starved fit81 mutant flies do not show any defects in detecting protein food and display normal preference for tryptone-supplemented (as a source of amino-acids) sucrose food over a sucrose-only food as controls, adult flies of both sexes also show no significant differences in body weight protein or lipid content compared to controls. However, in fit81 mutant adult females pre-feeding with tryptone leads to significantly weaker suppression of feeding (on standard food) compared to controls (pre-feeding with tryptone does not have a strong suppressive effect on feeding in males, either wild-type of fit81 mutant), pre-feeding with standard fly food or sucrose exerts normal feeding suppression in both females and males.
In a two-choice assay between sucrose-only and tryptone-supplemented food on unstarved flies, the fit81 mutants display similar feeding choices as the wild-type controls but the female mutant flies fail to adjust their feeding choice after tryptone pre-feeding and their preference for protein food remains high.
fit81 is rescued by fitUAS.Tag:HA/Scer\GAL4fit.PS
Expression of fitScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4fit.PS restores the disrupted suppressive effect of tryptone pre-feeding on feeding (on standard food) in fit81 adult females.