Thermogenetic activation (using TrpA1^UAS.(B).cKa at 30[o]C) of Scer\GAL4^Ilp2.PU cells in adults significantly reduces food intake of starved, but not fed, flies, compared to controls.

Inhibition (using Hsap\KCNJ2^UAS.cUa.EGFP) of Scer\GAL4^Ilp2.PU cells, in combination with a Gal80[ts] transgene to restrict expression to the few days of the adult stage prior to testing, does not affect food intake of fed or starved flies, compared to controls.

Optogenetic activation (using Crei\ChR1::Vcar\ChR1::Vcar\ChR2^{ReaChR.UAS.Citrine} with orange light) of the combined set of Scer\GAL4^Gr28b.b.4.7 and Scer\GAL4^Ilp2.PU cells, but not Scer\GAL4^Ilp2.PU cells alone, in adults significantly increases food intake of satiated flies, compared to controls, but this is to a significantly reduced extent compared to Scer\GAL4^Gr28b.b.4.7 cells alone.

Ablation of insulin-producing cells expressing Scer\GAL4^Ilp2.PU results in a higher mating success rate compared to controls, and the time to copulation is reduced.

Electrical silencing of insulin-producing cells expressing Scer\GAL4^Ilp2.PU (limited to the adult cells using Scer\GAL80^ts.αTub84B) has no effect on the mating success rate compared to controls, but the time to copulation is reduced.

Blocking neurosecretion in insulin-producing cells expressing Scer\GAL4^Ilp2.PU has no effect on the mating success rate compared to controls, but the time to copulation is reduced.