FlyBase Allele Report: Dmel\SkpA[GD9251]

General Information

Symbol

Dmel\SkpA^GD9251

Species

D. melanogaster

Name

FlyBase ID

FBal0210595

Feature type

allele

Associated gene

Dmel\SkpA

Associated Insertion(s)

Carried in Construct

P{GD9251}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD9251}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD9251

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

abnormal neuroanatomy | pupal stage, with Scer\GAL4^ppk.PG

(Wong et al., 2013)

lethal, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

lethal, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

lethal, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

larval dorsal multidendritic neuron ddaC | pupal stage, with Scer\GAL4^ppk.PG

(Wong et al., 2013)

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

Detailed Description

Statement

Reference

Expression of skpA^GD9251 under the control of two copies of Scer\GAL4^ppk.PG produces dendrite pruning defects in ddaC neurons at 16 hours after puparium formation. No difference is seen at the white prepupal stage.

(Wong et al., 2013)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in mild defects in the shape of neuroblasts in the larval brain.

(Neumüller et al., 2011)

Adults expressing SkpA^GD9251 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Adults expressing SkpA^GD9251 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in early pupal lethality or late larval lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality before the pupal stage.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

9251

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ppk.PG, SkpA^GD9251 has abnormal neuroanatomy | pupal stage phenotype, enhanceable by InR^K1409A.UAS, Scer\GAL4^ppk.PG

(Wong et al., 2013)

Suppressed by

Statement

Reference

Scer\GAL4^ppk.PG, SkpA^GD9251 has abnormal neuroanatomy | pupal stage phenotype, suppressible | partially by InR^K1409A.UAS, Scer\GAL4^ppk.PG

(Wong et al., 2013)

NOT suppressed by

Statement

Reference

Scer\GAL4^ppk.PG, SkpA^GD9251 has abnormal neuroanatomy | pupal stage phenotype, non-suppressible by Mical^N-ter.UAS, Scer\GAL4^ppk.PG

(Wong et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ppk.PG, SkpA^GD9251 has larval dorsal multidendritic neuron ddaC | pupal stage phenotype, enhanceable by InR^K1409A.UAS, Scer\GAL4^ppk.PG

(Wong et al., 2013)

Suppressed by

Statement

Reference

Scer\GAL4^ppk.PG, SkpA^GD9251 has larval dorsal multidendritic neuron ddaC | pupal stage phenotype, suppressible | partially by InR^K1409A.UAS, Scer\GAL4^ppk.PG

(Wong et al., 2013)

NOT suppressed by

Statement

Reference

Scer\GAL4^ppk.PG, SkpA^GD9251 has larval dorsal multidendritic neuron ddaC | pupal stage phenotype, non-suppressible by Mical^N-ter.UAS, Scer\GAL4^ppk.PG

(Wong et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Mical^{N-ter.Scer\UAS} does not suppress the dendrite pruning defects seen in ddaC neurons expressing skpA^GD9251 under the control of Scer\GAL4^ppk.PG at 16 hours after pupariation.

Expression of InR^{K1409A.Scer\UAS} suppresses the dendrite pruning defects seen in ddaC neurons expressing skpA^GD9251 under the control of Scer\GAL4^ppk.PG at 16 hours after pupariation.

Expression of InR^{A1325D.Scer\UAS} enhances the dendrite pruning defects seen in ddaC neurons expressing skpA^GD9251 under the control of Scer\GAL4^ppk.PG at 16 hours after pupariation.

(Wong et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 2 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

9251

Neuroblasts Screen Database (Knoblich Lab) - A database for RNAi phenotypes in larval neural stem cells

9251

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

SkpA^GD9251

skpA^GD9251

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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