FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\Nedd8
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General Information
Symbol
Dmel\Nedd8
Species
D. melanogaster
Name
Nedd8 ubiquitin like modifier
Annotation Symbol
CG10679
Feature Type
protein_coding_gene
FlyBase ID
FBgn0032725
Gene Model Status
Current
Stock Availability
13 publicly available
Gene Summary
Nedd8 (Nedd8) encodes a ubiquitin-like polypeptide involved in protein ubiquitination and degradation, via Cullin neddylation. Nedd8-mediated modification has been implicated in signal transduction, cell proliferation and differentiation. [Date last reviewed: 2018-10-11] (FlyBase Gene Snapshot)
All Summaries
Key Links
Genomic Location
Cytogenetic map
37B7-37B7
Sequence location
Recombination map
2-54
RefSeq locus
NT_033779 REGION:18951006..18951837
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (13 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables protein tag activity
inferred from direct assay
(Ou et al., 2002)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables protein tag activity
inferred from biological aspect of ancestor with PANTHER:PTN000071508
(GO Reference Genome Project, 2011-)
non-traceable author statement
(Wu et al., 2005, Zhu et al., 2005)
Biological Process (10 terms)
Terms Based on Experimental Evidence (9 terms)
CV Term
Evidence
References
involved_in cell population proliferation
inferred from mutant phenotype
(Ou et al., 2002)
involved_in female germ-line stem cell population maintenance
inferred from mutant phenotype
(Pan et al., 2014)
involved_in negative regulation of smoothened signaling pathway
inferred from mutant phenotype
(Ou et al., 2002)
involved_in protein neddylation
inferred from mutant phenotype
(Rencus-Lazar et al., 2008)
involved_in regulation of protein stability
inferred from mutant phenotype
(Ou et al., 2002)
involved_in regulation of proteolysis
inferred from mutant phenotype
(Ou et al., 2002)
involved_in regulation of smoothened signaling pathway
inferred from mutant phenotype
(Ou et al., 2002)
involved_in regulation of ubiquitin-protein transferase activity
inferred from mutant phenotype
(Ou et al., 2002)
involved_in sleep
inferred from mutant phenotype
(Stavropoulos and Young, 2011)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
involved_in modification-dependent protein catabolic process
inferred from biological aspect of ancestor with PANTHER:PTN000071508
(GO Reference Genome Project, 2011-)
involved_in protein neddylation
inferred from biological aspect of ancestor with PANTHER:PTN002241342
(GO Reference Genome Project, 2011-)
involved_in regulation of proteolysis
inferred from biological aspect of ancestor with PANTHER:PTN002241342
(GO Reference Genome Project, 2011-)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
is_active_in cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN000071508, PANTHER:PTN002241342
(GO Reference Genome Project, 2011-)
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000071508
(GO Reference Genome Project, 2011-)
located_in nucleus
inferred from sequence or structural similarity with UniProtKB:P29595
(Gene Ontology Curators, 2002-)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
Belongs to the ubiquitin family. (Q9VJ33)
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Nedd8 (Nedd8) encodes a ubiquitin-like polypeptide involved in protein ubiquitination and degradation, via Cullin neddylation. Nedd8-mediated modification has been implicated in signal transduction, cell proliferation and differentiation. [Date last reviewed: 2018-10-11]
Gene Group (FlyBase)
NEDD8 PROTEINS -
Nedd8 is a ubiquitin-like protein that is conjugated to substrates through a cascade of E1 activating enzymes, E2 conjugating enzymes, and E3 ligases. Conjugation of Nedd8 to target proteins (neddylation) regulates their activity, stability, subcellular localization, or macromolecular interactions. The best-characterized substrates of neddylation are cullins, essential components of cullin-RING E3 ubiquitin-ligase complexes. Neddylation has been implicated in various cellular processes including cell cycle progression, metabolism, immunity, and tumorigenesis. (Adapted from PMID:28234446 and PMID:33025631.)
Pathway (FlyBase)
NEGATIVE REGULATORS OF HEDGEHOG SIGNALING PATHWAY -
Negative regulators of hedgehog signaling down-regulate the pathway, resulting in the repression of transcription of hh-responsive genes.
Protein Function (UniProtKB)
Ubiquitin-like protein which plays an important role in cell cycle control, embryogenesis and neurogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex Uba3-Ula1 and linkage to the E2 enzyme UbcE2M. Attachment of Nedd8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
(UniProt, Q9VJ33)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Nedd8 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VJ33)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.51

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Nedd8-RA
FBtr0081192
NM_136075
471
84
Nedd8-RB
FBtr0342928
NM_001299141
612
84
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Nedd8-PA
FBpp0080733
9.3
84
5.88
Q9VJ33
NP_609919
AAF53724
Nedd8-PB
FBpp0309711
9.3
84
5.88
Q9VJ33
NP_001286070
AHN54584
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

84 aa isoforms: Nedd8-PA, Nedd8-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Covalently attached to cullins.

(UniProt, Q9VJ33)
Post Translational Modification

Cleavage of precursor form is necessary for function.

(UniProt, Q9VJ33)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Nedd8 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.12

Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Nedd8 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 11 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 12 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Nedd8
Transgenic constructs containing regulatory region of Nedd8
Aberrations (Deficiencies and Duplications) ( 2 )
Inferred from experimentation ( 2 )
Gene disrupted in
Df(2L)Exel7073
(Ryder, 2004.4.26)
Df(2L)TW3
(Ou et al., 2002)
Inferred from location ( 5 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4insc-Mz1407
lethal (with Df(2L)TW3)
lethal | recessive
lethal - all die before end of larval stage | recessive
lethal - all die before end of pupal stage, with Scer\GAL4pnr-MD237
lethal - all die before end of pupal stage (with Nedd8AN015)
lethal - all die before end of pupal stage (with Nedd8AN024)
lethal - all die before end of pupal stage (with Nedd8EP2063)
lethal - all die during P-stage, with Scer\GAL4Mef2.PR
partially lethal, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
female sterile | germline clone
Other Phenotypes
Allele
abnormal neuroanatomy
abnormal neuroanatomy | larval stage, with Scer\GAL4insc-Mz1407
abnormal sleep, with Scer\GAL4elav-C155
some die during larval stage | recessive
some die during pupal stage, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
some die during pupal stage, with Scer\GAL4pnr-MD237
some die during pupal stage (with Nedd8AN015)
some die during pupal stage (with Nedd8AN024)
some die during pupal stage (with Nedd8EP2063)
Phenotype manifest in
Allele
actin cytoskeleton | adult stage | female, with Scer\GAL4VP16.mat.αTub67C
adult mushroom body
embryonic/larval salivary gland, with Scer\GAL4fkh.PH
eye disc | somatic clone
female germline stem cell | somatic clone
ganglion mother cell | larval stage, with Scer\GAL4insc-Mz1407
larval neuroblast | larval stage, with Scer\GAL4insc-Mz1407
male germline stem cell | adult stage, with Scer\GAL4Act5C.PU
neuron & gamma-lobe
nurse cell ring canal, with Scer\GAL4VP16.mat.αTub67C
ovary | germline clone
testis | adult stage, with Scer\GAL4Act5C.PU
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (22)
Hsap\NEDD8
14 of 14
Yes
Yes
Hsap\RPS27A
3 of 14
No
No
Hsap\UBB
3 of 14
No
No
4  
Hsap\RAD23A
2 of 14
No
No
1  
Hsap\RAD23B
2 of 14
No
No
Hsap\UBA52
2 of 14
No
No
1  
Hsap\UBC
2 of 14
No
No
5  
Hsap\UBL4A
2 of 14
No
No
1  
Hsap\UBL4B
2 of 14
No
No
1  
Hsap\FAU
1 of 14
No
No
Hsap\NEDD8-MDP1
1 of 14
No
Yes
Hsap\OASL
1 of 14
No
Yes
Hsap\RPS27AP5
1 of 14
No
No
Hsap\UBD
1 of 14
No
No
Hsap\UBQLN1
1 of 14
No
No
5  
Hsap\UBQLN2
1 of 14
No
No
8  
Hsap\UBQLN3
1 of 14
No
No
Hsap\UBQLN4
1 of 14
No
No
Hsap\UBQLNL
1 of 14
No
No
Hsap\UBTD1
1 of 14
No
No
1  
Hsap\UBTD2
1 of 14
No
No
Hsap\ZFAND4
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (34)
Rnor\Nedd8
14 of 14
Yes
Yes
Rnor\Rps27a
3 of 14
No
No
Rnor\Rad23a
2 of 14
No
No
Rnor\Rad23b
2 of 14
No
No
Rnor\Uba52
2 of 14
No
No
Rnor\Ubb
2 of 14
No
No
Rnor\Ubqln1
2 of 14
No
No
Rnor\Fau
1 of 14
No
No
Rnor\Isg15
1 of 14
No
Yes
Rnor\LOC687780
1 of 14
No
No
Rnor\LOC100360645
1 of 14
No
Yes
Rnor\LOC100363494
1 of 14
No
Yes
Rnor\LOC100910768
1 of 14
No
Yes
Rnor\Oas1a
1 of 14
No
Yes
Rnor\Oas1b
1 of 14
No
Yes
Rnor\Oas1d
1 of 14
No
Yes
Rnor\Oas1e
1 of 14
No
Yes
Rnor\Oas1i
1 of 14
No
Yes
Rnor\Oasl2
1 of 14
No
Yes
Rnor\Oasl
1 of 14
No
Yes
Rnor\RGD1562433
1 of 14
No
No
Rnor\Rps27a-ps1
1 of 14
No
No
Rnor\Ubbp4
1 of 14
No
No
Rnor\Ubc
1 of 14
No
No
Rnor\Ubd
1 of 14
No
No
Rnor\Ubl4a
1 of 14
No
No
Rnor\Ubl7
1 of 14
No
No
Rnor\Ubqln2
1 of 14
No
No
Rnor\Ubqln3
1 of 14
No
No
Rnor\Ubqln4
1 of 14
No
No
Rnor\Ubqlnl
1 of 14
No
No
Rnor\Ubtd1
1 of 14
No
No
Rnor\Ubtd2
1 of 14
No
No
Rnor\Zfand4
1 of 14
No
No
Mus musculus (laboratory mouse) (34)
Mmus\Nedd8
14 of 14
Yes
Yes
Mmus\Gm8797
3 of 14
No
No
Mmus\Rps27a
3 of 14
No
No
Mmus\Ubb
3 of 14
No
No
Mmus\Rad23a
2 of 14
No
No
Mmus\Rad23b
2 of 14
No
No
Mmus\Uba52
2 of 14
No
No
Mmus\Ubl4a
2 of 14
No
No
Mmus\Ubl4b
2 of 14
No
No
Mmus\Fau
1 of 14
No
No
Mmus\Gm4802
1 of 14
No
Yes
Mmus\Gm11808
1 of 14
No
No
Mmus\Gm44504
1 of 14
No
No
Mmus\Isg15
1 of 14
No
Yes
Mmus\Oas1a
1 of 14
No
Yes
Mmus\Oas1c
1 of 14
No
Yes
Mmus\Oas1d
1 of 14
No
Yes
Mmus\Oas1e
1 of 14
No
Yes
Mmus\Oas1f
1 of 14
No
Yes
Mmus\Oas1g
1 of 14
No
Yes
Mmus\Oas1h
1 of 14
No
Yes
Mmus\Oasl1
1 of 14
No
Yes
Mmus\Oasl2
1 of 14
No
Yes
Mmus\Ubc
1 of 14
No
No
Mmus\Ubd
1 of 14
No
No
Mmus\Ubl7
1 of 14
No
No
Mmus\Ubqln1
1 of 14
No
No
Mmus\Ubqln2
1 of 14
No
No
Mmus\Ubqln3
1 of 14
No
No
Mmus\Ubqln4
1 of 14
No
No
Mmus\Ubqln5
1 of 14
No
No
Mmus\Ubqlnl
1 of 14
No
No
Mmus\Ubtd1
1 of 14
No
No
Mmus\Ubtd2
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (12)
Xtro\nedd8
11 of 13
Yes
Yes
Xtro\clta
2 of 13
No
No
Xtro\ccno
1 of 13
No
Yes
Xtro\dnajc15
1 of 13
No
No
Xtro\erich1
1 of 13
No
No
Xtro\rps27a
1 of 13
No
No
Xtro\uba52
1 of 13
No
No
Xtro\ubb
1 of 13
No
No
Xtro\ubc
1 of 13
No
No
Xtro\ubl4a
1 of 13
No
No
Xtro\ubtd1
1 of 13
No
No
Xtro\ubtd2
1 of 13
No
No
Danio rerio (Zebrafish) (20)
Drer\nedd8
14 of 14
Yes
Yes
Drer\nedd8l
9 of 14
No
Yes
Drer\rad23ab
2 of 14
No
No
Drer\rps27a
2 of 14
No
No
Drer\uba52
2 of 14
No
No
Drer\ubb
2 of 14
No
No
Drer\zgc:56596
2 of 14
No
No
Drer\bag6
1 of 14
No
No
Drer\faub
1 of 14
No
No
Drer\isg15
1 of 14
No
No
Drer\rad23aa
1 of 14
No
No
Drer\rad23b
1 of 14
No
No
Drer\ubc
1 of 14
No
No
Drer\ubl7a
1 of 14
No
No
Drer\ubl7b
1 of 14
No
No
Drer\ubqln4
1 of 14
No
No
Drer\ubtd1a
1 of 14
No
No
Drer\ubtd1b
1 of 14
No
No
Drer\ubtd2
1 of 14
No
No
Drer\zfand4
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (17)
Cele\ned-8
14 of 14
Yes
Yes
Cele\F52C6.2
2 of 14
No
No
Cele\ubq-1
2 of 14
No
No
Cele\ubq-2
2 of 14
No
No
Cele\C16C8.4
1 of 14
No
No
Cele\C16C8.5
1 of 14
No
Yes
Cele\C16C8.11
1 of 14
No
No
Cele\C16C8.13
1 of 14
No
Yes
Cele\C16C8.14
1 of 14
No
Yes
Cele\F14D2.11
1 of 14
No
No
Cele\F34H10.1
1 of 14
No
No
Cele\F52C6.3
1 of 14
No
No
Cele\F52C6.4
1 of 14
No
No
Cele\K08C9.7
1 of 14
No
No
Cele\rad-23
1 of 14
No
No
Cele\rps-30
1 of 14
No
No
Cele\ubl-1
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (12)
Agam\AgaP_AGAP007913
12 of 12
Yes
Yes
Agam\AgaP_AGAP001969
3 of 12
No
No
Agam\AgaP_AGAP001970
3 of 12
No
No
Agam\AgaP_AGAP001971
3 of 12
No
No
Agam\AgaP_AGAP002599
3 of 12
No
No
Agam\AgaP_AGAP000733
2 of 12
No
No
Agam\AgaP_AGAP002389
2 of 12
No
No
Agam\AgaP_AGAP007927
2 of 12
No
No
Agam\AgaP_AGAP001747
1 of 12
No
No
Agam\AgaP_AGAP001757
1 of 12
No
No
Agam\AgaP_AGAP004245
1 of 12
No
No
Agam\AgaP_AGAP004294
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (51)
Atha\RUB3
7 of 13
Yes
Yes
Atha\RUB1
5 of 13
No
Yes
Atha\UBQ7
5 of 13
No
Yes
Atha\AT1G23410
3 of 13
No
No
Atha\AT1G53930
3 of 13
No
Yes
Atha\UBQ5
3 of 13
No
No
Atha\UBQ6
3 of 13
No
No
Atha\UBQ8
3 of 13
No
No
Atha\UBQ9
3 of 13
No
No
Atha\UBQ10
3 of 13
No
No
Atha\UBQ11
3 of 13
No
No
Atha\UBQ12
3 of 13
No
No
Atha\UBQ13
3 of 13
No
No
Atha\UBQ14
3 of 13
No
No
Atha\AT1G11970
2 of 13
No
Yes
Atha\AT2G17190
2 of 13
No
No
Atha\AT2G36170
2 of 13
No
No
Atha\AT4G03360
2 of 13
No
No
Atha\AT5G16090
2 of 13
No
No
Atha\DSK2
2 of 13
No
No
Atha\RAD23A
2 of 13
No
No
Atha\RAD23B
2 of 13
No
No
Atha\RAD23C
2 of 13
No
No
Atha\RAD23D
2 of 13
No
No
Atha\UBQ1
2 of 13
No
No
Atha\UBQ3
2 of 13
No
No
Atha\UBQ4
2 of 13
No
No
Atha\AT1G16960
1 of 13
No
No
Atha\AT1G31335
1 of 13
No
Yes
Atha\AT1G53400
1 of 13
No
No
Atha\AT1G53950
1 of 13
No
No
Atha\AT1G53980
1 of 13
No
No
Atha\AT2G32350
1 of 13
No
No
Atha\AT3G06455
1 of 13
No
No
Atha\AT4G01000
1 of 13
No
No
Atha\AT4G02950
1 of 13
No
Yes
Atha\AT4G03370
1 of 13
No
No
Atha\AT4G05230
1 of 13
No
No
Atha\AT4G05240
1 of 13
No
No
Atha\AT4G05250
1 of 13
No
No
Atha\AT4G05260
1 of 13
No
No
Atha\AT4G05270
1 of 13
No
No
Atha\AT4G05310
1 of 13
No
No
Atha\AT5G09340
1 of 13
No
No
Atha\AT5G24240
1 of 13
No
No
Atha\AT5G25270
1 of 13
No
No
Atha\AT5G42220
1 of 13
No
No
Atha\AT5G45740
1 of 13
No
No
Atha\AT7SL-1
1 of 13
No
No
Atha\EVE1
1 of 13
No
No
Atha\PI4K GAMMA 4
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (8)
Scer\RUB1
10 of 13
Yes
Yes
Scer\DSK2
2 of 13
No
No
Scer\RPS31
2 of 13
No
No
Scer\UBI4
2 of 13
No
No
Scer\MDY2
1 of 13
No
No
Scer\RAD23
1 of 13
No
No
Scer\RPL40A
1 of 13
No
No
Scer\RPL40B
1 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (10)
Spom\ned8
12 of 12
Yes
Yes
Spom\rhp23
2 of 12
No
No
Spom\SPAC589.10c
2 of 12
No
No
Spom\ubi3
2 of 12
No
No
Spom\dph1
1 of 12
No
No
Spom\SPAPB2B4.07
1 of 12
No
No
Spom\SPBC1E8.02
1 of 12
No
No
Spom\ubi1
1 of 12
No
No
Spom\ubi4
1 of 12
No
No
Spom\uep1
1 of 12
No
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Nedd8. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (12)
CG11700
5 of 13
RpS27A
5 of 13
Ubi-p63E
5 of 13
RpL40
4 of 13
Ubi-p5E
4 of 13
CG7215
2 of 13
CG10694
2 of 13
CG1172
1 of 13
CG3223
1 of 13
CG31528
1 of 13
Rad23
1 of 13
Ubqn
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    Nedd8GD12964
    exacerbates  tauopathy
    modeled by Hsap\MAPTR406W.UAS
    (Butzlaff et al., 2015)
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    NEDD8; NEDD8 ubiquitin like modifier
    14 of 14
    603171
        RPS27A; ribosomal protein S27a
        3 of 14
        191343
            UBB; ubiquitin B
            3 of 14
            UBIQUITIN B; UBB
                 
                Functional Complementation Data
                Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                Interactions
                Summary of Physical Interactions
                Summary of Genetic Interactions
                Interaction Browsers
                View in FlyBase Interactions Browser   View in MIST tool (external link)
                Please look at the allele data for full details of the genetic interactions
                Starting gene(s)
                Interaction type
                Interacting gene(s)
                Reference
                Nedd8
                suppressible
                Den1
                (Chan et al., 2008)
                Starting gene(s)
                Interaction type
                Interacting gene(s)
                Reference
                bam
                enhanceable
                Nedd8
                (Pan et al., 2014)
                fru
                enhanceable
                Nedd8
                (Goto et al., 2011)
                External Data
                Subunit Structure (UniProtKB)
                Covalently attached to cullins.
                (UniProt, Q9VJ33 )
                Linkouts
                BioGRID - A database of protein and genetic interactions.
                DroID - A comprehensive database of gene and protein interactions.
                MIST (protein-protein) - An integrated Molecular Interaction Database
                Pathways
                Signaling Pathways (FlyBase)
                Metabolic Pathways
                FlyBase
                External Links
                External Data
                Class of Gene
                Genomic Location and Detailed Mapping Data
                Chromosome (arm)
                2L
                Recombination map
                2-54
                Cytogenetic map
                37B7-37B7
                Sequence location
                FlyBase Computed Cytological Location
                Cytogenetic map
                Evidence for location
                37B7-37B7
                Limits computationally determined from genome sequence between P{EP}CG10413EP2164 and P{lacW}l(2)37Dbk16106&P{lacW}Catsupk05424
                Experimentally Determined Cytological Location
                Cytogenetic map
                Notes
                References
                Experimentally Determined Recombination Data
                Location

                2-54

                (FlyBase, 2016)

                2-53.6

                (Comeron et al., 2012)
                Left of (cM)
                Right of (cM)
                Notes
                Stocks and Reagents
                Stocks (13)
                Genomic Clones (16)
                 

                Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

                cDNA Clones (30)
                 

                Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

                cDNA clones, fully sequenced
                BDGP DGC clones
                Other clones
                  Drosophila Genomics Resource Center cDNA clones

                  For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                  cDNA Clones, End Sequenced (ESTs)
                  BDGP DGC clones
                    Other clones
                    RNAi and Array Information
                    Linkouts
                    DRSC - Results frm RNAi screens
                    Antibody Information
                    Laboratory Generated Antibodies
                     
                    Commercially Available Antibodies
                     
                    Cell Line Information
                    Publicly Available Cell Lines
                     
                      Other Stable Cell Lines
                       
                        Other Comments

                        Nedd8 interacts with members of the Cullin family and is essential for SCF activities in Drosophila.

                        (Lin et al., 2002)

                        Mutant larvae and adults show pleiotrophic terminal phenotypes.

                        (Lin et al., 2002)
                        Relationship to Other Genes
                        Source for database merge of
                        Additional comments
                        Nomenclature History
                        Source for database identify of

                        Source for identity of: Nedd8 CG10679

                        (Lin, 2002.3.18)
                        Nomenclature comments
                        Etymology
                        Synonyms and Secondary IDs (8)
                        Reported As
                        Symbol Synonym
                        CG10679
                        (National Institute of Genetics Fly Stocks, 2023-, National Institute of Genetics Fly Stocks, 2022-, Pirone et al., 2017, Butzlaff et al., 2015, Hallier et al., 2015, Hosono et al., 2015, Ni et al., 2010.12.1, Sepp et al., 2008)
                        NEDD8
                        (Kim et al., 2017, Lu et al., 2013, Broemer et al., 2010)
                        Nedd
                        (Neukomm et al., 2017)
                        Nedd8
                        (Kagemann et al., 2025, Bosch et al., 2023, Olivares et al., 2023, Amstutz et al., 2022, National Institute of Genetics Fly Stocks, 2022-, Barbosa et al., 2021, Sureka and Mishra, 2021, Lin et al., 2020, Suisse et al., 2018, Nagarkar-Jaiswal et al., 2017, Pirone et al., 2017, Fu et al., 2016, Liu et al., 2016, Park et al., 2016, Sarov et al., 2016, Yu et al., 2016, Butzlaff et al., 2015, Hudson et al., 2015, Kavi et al., 2015, Lu et al., 2015, Gonzalez et al., 2014, Huang et al., 2014, Pan et al., 2014, Kroeger et al., 2013, Strutt et al., 2013, Goto et al., 2011, Neumüller et al., 2011, Stavropoulos and Young, 2011, Wu et al., 2011, Kim et al., 2010, Djagaeva and Doronkin, 2009, Chan et al., 2008, Chan et al., 2008, Rencus-Lazar et al., 2008, Reynolds et al., 2008, Sepp et al., 2008, Engström et al., 2007, Ou et al., 2007, Liebl et al., 2006, Wu et al., 2005, Zhu et al., 2005, Cope et al., 2002)
                        dNEDD8
                        (Du et al., 2011)
                        nedd8
                        (Hallier et al., 2015, Lu et al., 2015, Blount et al., 2014, Christiansen et al., 2013, Wong et al., 2013, Maybeck and Röper, 2009, Oren-Giladi et al., 2008, Wu and Chien, 2004)
                        Name Synonyms
                        Nedd8
                        Nedd8 ubiquitin like modifier
                        (Shafer, 2025)
                        neural precursor cell-expressed developmentally downregulated
                        (Zhu et al., 2005)
                        Secondary FlyBase IDs
                          Datasets (0)
                          Study focus (0)
                          Experimental Role
                          Project
                          Project Type
                          Title
                          Study result (0)
                          Result
                          Result Type
                          Title
                          External Crossreferences and Linkouts ( 40 )
                          Sequence Crossreferences
                          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                          UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                          UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                          UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                          Other crossreferences
                          AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                          BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                          DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                          EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                          FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                          FlyMine - An integrated database for Drosophila genomics
                          InterPro - A database of protein families, domains and functional sites
                          KEGG Genes - Molecular building blocks of life in the genomic space.
                          MARRVEL_MODEL - MARRVEL (model organism gene)
                          Linkouts
                          BioGRID - A database of protein and genetic interactions.
                          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                          DroID - A comprehensive database of gene and protein interactions.
                          DRSC - Results frm RNAi screens
                          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                          Flygut - An atlas of the Drosophila adult midgut
                          FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                          MIST (protein-protein) - An integrated Molecular Interaction Database
                          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                          References (92)