Gene model reviewed during 5.47
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.56
The coding sequence for the 52aa ribosomal protein is fused to a ubiquitin coding sequence in the Drosophila genome. The 52aa ribosomal protein was shown to be released from the fusion protein by proteolytic processing and was detected by antibodies prepared against the ribosomal portion of the protein. The apparent proteolytic processing was confirmed by amino-terminal sequence analysis. The 52aa protein is associated with the large ribosomal subunit.
60S ribosomal protein is part of the 60S ribosomal subunit.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RpL40 using the Feature Mapper tool.
RpL40 transcripts are detected throughout development on northern blots. They are also detected in RNA from dissected tissues, namely larval Malpighian tubules and intestines, imaginal discs, fat body, salivary gland, and adult ovary. The level in ovaries is at least four times higher than that of the other dissected tissues.
GBrowse - Visual display of RNA-Seq signalsView Dmel\RpL40 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Likely Minute gene.
Deletions removing RpL40 but no other cytoplasmic ribosomal protein-encoding genes show Minute phenotypes.
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
Antibody staining of polytene chromosomes shows that ubiquitin is mainly associated with the compact and stabilized structure that forms the bands rather than with the more decondensed and destabilized protein-DNA structure that forms interbands and puffs.
Ubi-f52 gene organization and expression has been determined.