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Christensen, S., Cook, K. (2006.12.5). Isolation and characterization of Df(2L)BSC217. 
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To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(2L)BSC217
Cc: Jill Gresens <jgresensXXXX>, Stacey Christensen <sjchristXXXX>, mdealXXXX, kaufmanXXXX
Isolation and characterization of Df(2L)BSC217
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC217 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d09957 and PBac{WH}CG3058[f03902]. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; P{XP}d09957/PBac{WH}CG3058[f03902] males 
crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.WH5}BSC217 from the segment 
of P{XP}d09957 to the left of its FRT site and the segment of 
PBac{WH}CG3058[f03902] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
Exelixis, Inc. determined the insertion site of 
PBac{WH}CG3058[f03902] to be at Release 3 genomic coordinate 4395974 
on chromosome arm 2L, a site predicted to be within 24F1 on both the 
Release 3 and Release 4 genome maps. The predicted position of 
P{XP}d09957 on the Release 4 map is 24D8. Consequently, the predicted 
cytological breakpoints of Df(2L)BSC217 are 24D8;24F1. The presence 
of a deletion was confirmed cytologically, though the breakpoints 
were not analyzed in detail. Df(2L)BSC217 heterozygosity gives rise 
to a Minute bristle phenotype from deletion of RpL40.
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology 
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX 
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    Aberrations (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (1)