Isolation and characterization of Df(2L)BSC217
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC217 was isolated as a FLP recombinase-induced recombination event involving P{XP}d09957 and PBac{WH}CG3058f03902. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}d09957/PBac{WH}CG3058f03902 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC217 from the segment of P{XP}d09957 to the left of its FRT site and the segment of PBac{WH}CG3058f03902 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of PBac{WH}CG3058f03902 to be at Release 3 genomic coordinate 4395974 on chromosome arm 2L, a site predicted to be within 24F1 on both the Release 3 and Release 4 genome maps. The predicted position of P{XP}d09957 on the Release 4 map is 24D8. Consequently, the predicted cytological breakpoints of Df(2L)BSC217 are 24D8;24F1. The presence of a deletion was confirmed cytologically, though the breakpoints were not analyzed in detail. Df(2L)BSC217 heterozygosity gives rise to a Minute bristle phenotype from deletion of RpL40.