FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Den1, Nedd8
FlyBase ID
FBig0000095803
Interaction Network
Interactions Browser links
Den1 network
Nedd8 network
Reported Interactions
FBrf0205968-1.PD.WB
Reference
Chan et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Den1 

DEN1

bait

GST tag

Nedd8 

Nedd8

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed and purified from bacteria.

(Chan et al., 2008)

Prey expressed and purified from bacteria.

(Chan et al., 2008)

Den1 binds the ubiquitin-like Nedd8, but not ubiquitin or the ubiquitin-like smt3 (SUMO).

(Chan et al., 2008)

GST pull down.

(Chan et al., 2008)
FBrf0205968-2.ENZ.WB
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Den1 

DEN1

enzyme
Den1 

DEN1

enzyme
Den1 
Nedd8 

mNedd8

enzyme target

His tag

Nedd8 

mNedd8

enzyme target

His tag

Nedd8 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)