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Citation
Stavropoulos, N., Young, M.W. (2011). insomniac and Cullin-3 Regulate Sleep and Wakefulness in Drosophila.  Neuron 72(6): 964--976.
FlyBase ID
FBrf0217043
Publication Type
Research paper
Abstract

In a forward genetic screen in Drosophila, we have isolated insomniac, a mutant that severely reduces the duration and consolidation of sleep. Anatomically restricted genetic manipulations indicate that insomniac functions within neurons to regulate sleep. insomniac expression does not oscillate in a circadian manner, and conversely, the circadian clock is intact in insomniac mutants, suggesting that insomniac regulates sleep by pathways distinct from the circadian clock. The protein encoded by insomniac is a member of the BTB/POZ superfamily, which includes many proteins that function as adaptors for the Cullin-3 (Cul3) ubiquitin ligase complex. We show that Insomniac can physically associate with Cul3, and that reduction of Cul3 activity in neurons recapitulates the insomniac phenotype. The extensive evolutionary conservation of insomniac and Cul3 suggests that protein degradation pathways may have a general role in governing the sleep and wakefulness of animals.

PubMed ID
PubMed Central ID
PMC3244879 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Neuron
    Title
    Neuron
    Publication Year
    1988-
    ISBN/ISSN
    0896-6273
    Data From Reference
    Aberrations (1)
    Alleles (35)
    Genes (10)
    Physical Interactions (1)
    Cell Lines (1)
    Natural transposons (1)
    Insertions (17)
    Experimental Tools (3)
    Transgenic Constructs (20)
    Transcripts (1)