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General Information
Symbol
Dmel\incf00285
Species
D. melanogaster
Name
FlyBase ID
FBal0162225
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
inc2
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion in the 5' untranslated region. The inserted PBac{WH} element contains Scer\UAS regulatory sequences that are in the correct orientation to over-express inc in the presence of a Scer\GAL4 driver.

Insertion components
PBac{WH}incf00285
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

incf00285 mutant third instar larvae show increased number of boutons on neuromuscular junction. Postsynaptic muscle recordings reveal altered synaptic transmission in inc1/incf00285 larvae: the amplitude of spontaneous miniature postsynaptic potentials is comparable to controls but their frequency is reduced, the amplitude of evoked potentials is decreased as is the quantal content.

Mutant flies show a reduction in sleep duration compared to controls. Sleep is reduced during both light and dark periods, but the sleep levels of the mutant flies drop in anticipation of light-dark and dark-light conditions, suggesting that the circadian clock is intact. The mutant flies show a reduction in sleep bout length accompanied by an increase in sleep bout number and show an increased latency to sleep after lights-off. The mutant flies show a modest reduction in activity during wakefulness. The mutant flies show reduced sleep homeostasis, lacking a detectable sleep rebound after 12 or 24 hours of sleep deprivation. The mutant flies are more easily woken by a rotational stimulus compared to wild type controls.

A proportion of mutant flies lack either an α- or β-lobe in the mushroom body.

These homozygotes are also short lived compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Statement
Reference

incf00285 has abnormal sleep phenotype, non-suppressible by Cdk1[+]/Cdk1GT-000294

Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

One copy of Cdk1GT-000294 does not suppress the sleep defects seen in incf00285 mutant flies.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Fails to complement
Partially rescued by
Not rescued by
Partially rescues
Fails to rescue
Comments

Expression of inc under the control of either Scer\GAL4inc.PP or Scer\GAL4Cha.7.4 from the Scer\UAS regulatory sequences present in the PBac{WH}incf00285 insertion rescue the defects in sleep duration seen in incf00285 mutants, while expression under the control of either Scer\GAL430Y, Scer\GAL4c309 or Scer\GAL4G0451 partially rescues the sleep duration defects. Expression under the control of Scer\GAL4Mef2.247 does not rescue the sleep duration defects. Expression under the control of either Scer\GAL430Y or Scer\GAL4Cha.7.4 rescues the sleep homeostasis defects of incf00285 mutants.

Expression of inc under the control of Scer\GAL4elav.Switch.PO during development (using RU486 to control the timing of expression) from the Scer\UAS regulatory sequences present in the PBac{WH}incf00285 insertion partially rescues the sleep defects of incf00285 mutants, but expression under the control of Scer\GAL4elav.Switch.PO during the adult stage alone does not result in significant rescue of the sleep defects.

DATfmn incf00285 double mutants do not sleep significantly less than either single mutant.

Expression of inc from the PBac{WH}incf00285 insertion (using either Scer\GAL4Act5C.PI or Scer\GAL4αTub84B.PL) completely rescues the reduction in sleep per day which is seen in incf00285 homozygotes.

Expression of inc from the PBac{WH}incf00285 insertion using any one of the following Scer\GAL4 drivers partially rescues the reduction in sleep per day which is seen in incf00285 homozygotes: Scer\GAL4n-syb.PS, Scer\GAL4qvr.PW, Scer\GAL4Cha.7.4, Scer\GAL4D42, Scer\GAL4inc.PS, Scer\GAL4VGlut.PS, Scer\GAL4c309 and Scer\GAL4cenB1A-Mai301.

Expression of inc from the PBac{WH}incf00285 insertion using any one of the following Scer\GAL4 drivers does not rescue the reduction in sleep per day which is seen in incf00285 homozygotes: Scer\GAL4c739, Scer\GAL4tim.PE, Scer\GAL4cry.PU, Scer\GAL4Ilp2.PR, Scer\GAL430Y, Scer\GAL417d, Scer\GAL4ey-OK107, Scer\GAL4Tab2-201Y, Scer\GAL4Aph-4-c507, Scer\GAL4Tdc2.PC, Scer\GAL4ple.PF, Scer\GAL4Mef2.PR, Scer\GAL4how-24B or Scer\GAL4repo.PU.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (8)