Comment: innervating abdominal 6 dorsal muscle 133
Drives expression in a subset of octopaminergic and tyraminergic neurons.
FLP/FRT intersectional strategy shows expression of Scer\GAL4Tdc2.PC in a subset of cell bodies in the posterior part of the thoracico-abdominal ganglion when combined with dsxKI.FLPG5. On average nine neurons are labeled in females and three in males. In females these project to the spermathecum, the seminal receptacle, the female accessory gland, the uterus and to both the common and lateral oviduct. In males projections lead to the testis, the ejaculatory duct and the male accessory gland. In both male and female expression can also be detected in a motor neuron that innervates abdominal 6 dorsal muscle 133.
Scer\GAL4Tdc2.PC drives expression in 44 neurons of the ventral nerve cord. In brain hemispheres labeling is observed in three distinct locations with one neuron located basomedial to the calyx, a cluster of four anterior to the vertical mushroom body lobes and around two cells dorsomedial to the antennal lobe. Scer\GAL4Tdc2.PC is detected in clusters of VM neurons in all three larval gnathal neuromeres and in one cell at the margin of the subesophageal ganglion to the ventral nerve cord. All Scer\GAL4Tdc2.PC-expressing neurons are tyraminergic, whereas labeled cells positioned in the subesophageal ganglion are octopaminergic as well.
Scer\GAL4Tdc2.PC drives expression in motor neurons that form type II terminal buttons at the neuromuscular junction.
Scer\GAL4Tdc2.PC drives expression in the adult octopaminergic neurons. Positive GRASP (GFP reconstitution across synaptic partners) signals between the mushroom body alpha and beta lobes labelled with Ecol\lexAL5076 or Ecol\lexAL5251 and Scer\GAL4Tdc2.PC are observed sporadically in the alpha and beta lobes and in the calyx.
Scer\GAL4Tdc2.PC drives expression in clusters of neurons in the adult brain and thoracico-abdominal ganglion. Co-localisation with Tbh protein in the brain is observed in the octopaminergic AL2 cluster, in the G3b and 3 ventral clusters (VMI to VMIII). In the thoracico-abdominal ganglion, 37 of the 39 Tbh-positive neurons also express Scer\GAL4Tdc2.PC.
Expression observed in neurons that innervate the antennal lobes, mushroom body gamma lobes and their spurs, the subesophageal ganglion, the areas surrounding the esophagus, and the mushroom body calyces.
Expression of Scer\GAL4Tdc2.PC is in both octopaminergic and tyraminergic neurons.
Scer\GAL4Tdc2.PC drives expression in several clusters of cells in the adult, in a total of 137 cells per hemisphere. These clusters include the octopaminergic neurons VM (27 cells), AL2 (7 cells), VL (2 cells) and ASM (8 cells). In addition, Scer\GAL4Tdc2.PC labels cells around the protocerebral bridge (PB1 cluster, 65 cells), ventral to the protocerebral bridge (PB2 cluster, 2 cells), posterior superior medial protocerebrum (PSM cluster, 5 cells) and at the anterior margin of the antennal lobe (AL1 cluster, 1 cell).
Expression is seen in discrete unilateral subsets of cells located along the ventral medial line of the brain, as well as in discrete
bilateral clusters of cells in the lateral protocerebrum region and surrounding the esophagus cavity.
In larva, Scer\GAL4Tdc2.PC drives expression in a few processes that invade the calyx of the mushroom body and in neurites that surround the peduncle.
Ablation (using hidUAS.cUa and rprUAS.cUa) of Scer\GAL4Tdc2.PC neurons in larvae results in a significant reduction in the distance they crawl and a partial circling behavior, relative to controls; when ablation is restricted to a smaller subset of cells, using Scer\GAL80tsh-GAL80, this effect is not seen.
Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 590 nm (yellow) light) of Scer\GAL4Tdc2.PC neurons suppresses the induction of pulse song observed due to optogenetic activation (using Crei\ChR1ReaChR.lexAop.FRT.mCherry.Citrine with 590 nm (yellow) light) of Ecol\lexAVT040556 neurons that express Scer\FLP1fru in adult males.