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General Information
Symbol
Scer\GAL4Tdc2.PC
Species
S. cerevisiae
Name
FlyBase ID
FBal0338174
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
TDC2-GAL4, Tdc-Gal4, dTdc2-Gal4, Tdc2 GAL4
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A Tdc2 promoter (-3459 to +4530 relative to the coding start site) drives GAL4 expression. A Hsp70 3' poly(A) sequence flanks the GAL4 coding region.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
motor neuron | subset of adult abdominal segment 6

Comment: innervating abdominal 6 dorsal muscle 133

Additional Information
Statement
Reference

Drives expression in a subset of octopaminergic and tyraminergic neurons.

FLP/FRT intersectional strategy shows expression of Scer\GAL4Tdc2.PC in a subset of cell bodies in the posterior part of the thoracico-abdominal ganglion when combined with dsxKI.FLPG5. On average nine neurons are labeled in females and three in males. In females these project to the spermathecum, the seminal receptacle, the female accessory gland, the uterus and to both the common and lateral oviduct. In males projections lead to the testis, the ejaculatory duct and the male accessory gland. In both male and female expression can also be detected in a motor neuron that innervates abdominal 6 dorsal muscle 133.

Scer\GAL4Tdc2.PC drives expression in 44 neurons of the ventral nerve cord. In brain hemispheres labeling is observed in three distinct locations with one neuron located basomedial to the calyx, a cluster of four anterior to the vertical mushroom body lobes and around two cells dorsomedial to the antennal lobe. Scer\GAL4Tdc2.PC is detected in clusters of VM neurons in all three larval gnathal neuromeres and in one cell at the margin of the subesophageal ganglion to the ventral nerve cord. All Scer\GAL4Tdc2.PC-expressing neurons are tyraminergic, whereas labeled cells positioned in the subesophageal ganglion are octopaminergic as well.

Scer\GAL4Tdc2.PC drives expression in motor neurons that form type II terminal buttons at the neuromuscular junction.

Scer\GAL4Tdc2.PC drives expression in the adult octopaminergic neurons. Positive GRASP (GFP reconstitution across synaptic partners) signals between the mushroom body alpha and beta lobes labelled with Ecol\lexAL5076 or Ecol\lexAL5251 and Scer\GAL4Tdc2.PC are observed sporadically in the alpha and beta lobes and in the calyx.

Scer\GAL4Tdc2.PC drives expression in clusters of neurons in the adult brain and thoracico-abdominal ganglion. Co-localisation with Tbh protein in the brain is observed in the octopaminergic AL2 cluster, in the G3b and 3 ventral clusters (VMI to VMIII). In the thoracico-abdominal ganglion, 37 of the 39 Tbh-positive neurons also express Scer\GAL4Tdc2.PC.

Expression observed in neurons that innervate the antennal lobes, mushroom body gamma lobes and their spurs, the subesophageal ganglion, the areas surrounding the esophagus, and the mushroom body calyces.

Expression of Scer\GAL4Tdc2.PC is in both octopaminergic and tyraminergic neurons.

The octopaminergic neurons VPM1 and VUM labelled with Scer\GAL4Tdc2.PC co-localise with male-specific fru protein (FruM).

Scer\GAL4Tdc2.PC drives expression in several clusters of cells in the adult, in a total of 137 cells per hemisphere. These clusters include the octopaminergic neurons VM (27 cells), AL2 (7 cells), VL (2 cells) and ASM (8 cells). In addition, Scer\GAL4Tdc2.PC labels cells around the protocerebral bridge (PB1 cluster, 65 cells), ventral to the protocerebral bridge (PB2 cluster, 2 cells), posterior superior medial protocerebrum (PSM cluster, 5 cells) and at the anterior margin of the antennal lobe (AL1 cluster, 1 cell).

Expression is seen in discrete unilateral subsets of cells located along the ventral medial line of the brain, as well as in discrete

bilateral clusters of cells in the lateral protocerebrum region and surrounding the esophagus cavity.

In larva, Scer\GAL4Tdc2.PC drives expression in a few processes that invade the calyx of the mushroom body and in neurites that surround the peduncle.

Scer\GAL4Tdc2.PC drives expression in adult thoracic and abdominal neuromeres. Their cell bodies are located at the midline extending bilateral symmetric axons as well as a net of finer processes.

 
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 590 nm (yellow) light) of Scer\GAL4Tdc2.PC neurons in adult males results in a significant suppression of pulse song in the presence of a female and a significant increase in wing beat amplitude during flight, compared to controls; both of these effects are also seen when the number of activated neurons is restricted using Scer\FLP1FLPo.oc.Tag:NLS(SV40-largeT) and Scer\GAL80FRT.αTub84B, but with a smaller suppression of pulse song and no suppression of pulse amplitude; full pulse song suppression, but no wing beat amplitude increase, occurs when the number of activated neurons is restricted using Scer\GAL80tsh-GAL80.

Thermogenetic activation (using TrpA1UAS.(B).cKa at 30[o]C) of Scer\GAL4Tdc2.PC neurons in adult males results in a significant suppression of pulse song in the presence of a female.

Inhibition (using Hsap\KCNJ2IVS.10xUAS.EGFP) of Scer\GAL4Tdc2.PC cells in adults significantly reduces turning responses to fast-moving light stimuli and longer-duration, slow-moving light stimuli, compared to controls, with no effect on the speed of forward locomotion.

Inhibition (using shi1.UAS at 32[o]C) of Scer\GAL4Tdc2.PC cells in adults does not affect 5 min (single-trial conditioned) aversive taste memory, compared to controls.

Inhibition of neural activity in Scer\GAL4Tdc2.PC-expressing neurons does not affect sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503). As in wild type flies, courtship defects are seen in response to (S,Z,Z)-CH503.

Activation of Tdc-positive neurons by expressing TrpA1Scer\UAS.(B).cKa under the control of Scer\GAL4Tdc2.PC at the permissive temperature induces post-mating behaviors in virgin females. These include reduced levels of copulation within one hour, increased rejection behaviors (ovipositor extrusion) and increased egg laying.

Activation of Tdc and dsx-positive neurons by expressing TrpA1Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Tdc2.PC (in which the Scer\FRT cassette has been excised by Scer\FLP1dsx) at the permissive temperature reduces receptivity in virgin females. Only a small percentage of females copulate within 1 hour and they show increased rejection behaviors (ovipositor extrusion) and egg laying compared to controls.

Silencing Tdc-positive neurons by expressing Ctet\TeTxLCTNT.Scer\UAS under the control of Scer\GAL4Tdc2.PC at the permissive temperature inhibits post-mating behaviors in mated females, including increased levels of remating, reduced rejection behaviors (ovipositor extrusion) and reduced egg laying. These mated females remain highly attractive to males.

Silencing of Tdc and dsx-positive neurons by expressing Ctet\TeTxLCScer\UAS.cSa under the control of Scer\GAL4Tdc2.PC (in which the Scer\FRTcassette has been excised by Scer\FLP1dsx) at the permissive temperature results in disrupted post-mating practices in mated females. These females are more receptive than wild type females, as they remate at a significantly higher frequency and show decreased levels of rejection behaviors (ovipositor extrusion). In addition, they elicit more male courtship and show decreased levels of egg laying.

Inhibition (using shi1.UAS at 33[o]C) of Scer\GAL4Tdc2.PC cells in adults does not significantly affect appetitive or aversive 1-min visual memory, compared to controls.

Blocking synaptic transmission (using Hsap\KCNJ2UAS.EGFP) of Scer\GAL4Tdc2.PC neurons in larvae results in a significant reduction in the distance they crawl and a partial circling behavior, relative to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
Suppressor of
Phenotype Manifest In
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Ablation (using hidUAS.cUa and rprUAS.cUa) of Scer\GAL4Tdc2.PC neurons in larvae results in a significant reduction in the distance they crawl and a partial circling behavior, relative to controls; when ablation is restricted to a smaller subset of cells, using Scer\GAL80tsh-GAL80, this effect is not seen.

Xenogenetic Interactions
Statement
Reference

Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 590 nm (yellow) light) of Scer\GAL4Tdc2.PC neurons suppresses the induction of pulse song observed due to optogenetic activation (using Crei\ChR1ReaChR.lexAop.FRT.mCherry.Citrine with 590 nm (yellow) light) of Ecol\lexAVT040556 neurons that express Scer\FLP1fru in adult males.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Scer\GAL4Tdc.PJ
Scer\GAL4Tdc2.PC
Name Synonyms
Tdc2 promoter construct of Cole
Tyrosine decarboxylase promoter construct of Johard
Secondary FlyBase IDs
  • FBal0190372
  • FBal0287826
References (116)