FlyBase Allele Report: Dmel\twz[KK107922]

General Information

Symbol

Dmel\twz^KK107922

Species

D. melanogaster

Name

FlyBase ID

FBal0259576

Feature type

allele

Associated gene

Dmel\twz

Associated Insertion(s)

Carried in Construct

P{KK107922}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK107922}

Cytology

Description

Male flies expressing twz^KK107922 via Scer\GAL4^Tdc2.PC at 29[o]C have only 0.33-fold of the normal twz RNA expression levels and only 0.42-fold of normal TfAP-2 expression. That is, expression of twz^KK107922 reduces the level of both twz and TfAP-2.

(Williams et al., 2014)

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK107922

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | male limited, with Scer\GAL4^Tdc2.PC

(Williams et al., 2014)

abnormal courtship behavior | male limited, with Scer\GAL4^Tdc2.PC

(Williams et al., 2014)

abnormal feeding behavior | male limited, with Scer\GAL4^Tdc2.PC

(Williams et al., 2014)

abnormal locomotor behavior | male limited, with Scer\GAL4^Tdc2.PC

(Williams et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

Scer\GAL4^Tdc2.PC twz^KK107922 males show similar high intensity fighting behaviours, but increased low intensity fighting compared to controls in male aggression assays.

Scer\GAL4^Tdc2.PC twz^KK107922 males show an increased frequency of mating behaviours towards other males or towards virgin females compared to controls.

Scer\GAL4^Tdc2.PC twz^KK107922 males do not show any change in their overall activity, though they walk at a significantly slower pace compared to controls.

(Williams et al., 2014)

Scer\GAL4^Tdc2.PC twz^KK107922 male flies over-eat after starvation compared to controls.

Scer\GAL4^Tdc2.PC twz^KK107922 male flies eat significantly more food ad lib than controls in a 24 hr period. The number of feeding bouts is unaffected, while meal size is slightly increased.

(Williams et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

twz^KK107922/Scer\GAL4^Tdc2.PC is a suppressor of abnormal behavior | male limited phenotype of Scer\GAL4^Tdc2.PC, TfAP-2^B.UAS

(Williams et al., 2014)

twz^KK107922/Scer\GAL4^Tdc2.PC is a suppressor of abnormal locomotor behavior | male limited phenotype of Scer\GAL4^Tdc2.PC, TfAP-2^B.UAS

(Williams et al., 2014)

twz^KK107922/Scer\GAL4^Tdc2.PC is a suppressor of abnormal feeding behavior | male limited phenotype of Scer\GAL4^Tdc2.PC, TfAP-2^B.UAS

(Williams et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of twz^KK107922 rescues the increased high intensity fighting and hyperactive phenotype of Scer\GAL4^Tdc2.PC TfAP-2^B.Scer\UAS male flies.

(Williams et al., 2014)

Co-expression of twz^KK107922 suppresses the over-eating phenotypes seen in Scer\GAL4^Tdc2.PC TfAP-2^B.Scer\UAS male flies.

(Williams et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v110265

P{KK107922}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG10440^KK107922

twz^KK107922

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close