FlyBase Allele Report: Dmel\Adar[5G1]

General Information

Symbol

Dmel\Adar^5G1

Species

D. melanogaster

Name

FlyBase ID

FBal0118605

Feature type

allele

Associated gene

Dmel\Adar

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Palladino et al., 2000)

Mutagen

P-element activity

(Palladino et al., 2000)

Progenitor genotype

Adar^HD57

(Palladino et al., 2000)

Cytology

Description

Imprecise excision of the P-element, deleting genomic DNA unidirectionally in the 3' direction, removing all Adar coding sequences.

(Palladino et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | adult stage | recessive | heat sensitive

(Palladino et al., 2000)

abnormal courtship behavior | male

(Palladino et al., 2000)

abnormal flight | recessive

(Palladino et al., 2000)

abnormal grooming behavior | recessive

(Palladino et al., 2000)

abnormal locomotor behavior

(Li et al., 2014, Keegan et al., 2005)

abnormal locomotor behavior | adult stage

(Deng et al., 2020, Keegan et al., 2011)

abnormal locomotor behavior | adult stage | recessive | heat sensitive

(Palladino et al., 2000)

abnormal locomotor rhythm

(Jepson et al., 2011)

abnormal neurophysiology | larval stage

(Li et al., 2014)

lethal, with Adar^3.4.S.UAS, Scer\GAL4^Mef2.PR

(Keegan et al., 2005)

lethal | conditional | recessive

(Palladino et al., 2000)

lethal - all die before end of P-stage, with Adar^3.4.S.UAS, Scer\GAL4^Act5C.PI

(Li et al., 2014)

paralytic | adult stage | recessive | heat sensitive

(Palladino et al., 2000)

uncoordinated

(Palladino et al., 2000)

uncoordinated | heat sensitive

(Palladino et al., 2000)

viable, with Adar^3.4.S.UAS, Scer\GAL4^ChAT.7.4

(Keegan et al., 2005)

Phenotype Manifest In

adult brain

(Deng et al., 2020)

larval DA1 motor neuron | larval stage

(Li et al., 2014)

neuromuscular junction

(Bhogal et al., 2011)

retina | adult stage

(Deng et al., 2020)

synapse

(Bhogal et al., 2011)

type I bouton

(Bhogal et al., 2011)

wing

(Palladino et al., 2000)

Detailed Description

Statement

Reference

Expression of Adar^{3.4.S.Scer\UAS} under the control of Scer\GAL4^Act5C.PI in a hemizygous Adar^5G1 background leads to lethality in larval and pupal stages.

Adar^5G1 mutant flies display locomotion and climbing defects, and larvae exhibit aCC neuron hyperactivity.

(Li et al., 2014)

RNA editing is abolished in Adar^5G1 mutants (assayed through RNA editing of para).

(Rieder et al., 2013)

Adar^5G1 mutant larvae exhibit a 59% and 35% increase in the total number of type I synaptic boutons compared with wild-type in muscles 6/7 and muscle 4, respectively. Quantification of branching in muscles 6/7 reveals that Adar^5G1 mutants exhibit an increase in branching compared with control samples. The increase in type I synaptic boutons in Adar^5G1 mutants arises largely from an increase in type 1s synaptic boutons, whereas type 1b synaptic boutons are subtly affected, if at all, compared to controls.

Adar^5G1 mutant third instar larvae exhibit an increase in type Is and to a lesser extent type 1b synaptic boutons and branching compared to wild-type controls.

(Bhogal et al., 2011)

Adar^5G1 males lack coordinated locomotor pattern.

(Jepson et al., 2011)

Mutant adults show a neurodegeneration phenotype, with vacuolisation being seen in the retina and the mushroom body calyces at 30 days of age.

(Keegan et al., 2011)

Under ideal growth conditions, homozygotes develop into morphologically normal adults with profound behavioural deficits. Mutant males rarely successfully mate with wild-type females. When presented with wild-type or Adar^- females, mutant males do not initiate any displays of courtship. Homozygous females can be mated by wild-type males and give rise to morphologically normal hemizygous males which show the mutant adult behavioural defects. Mutant adults show severe neuro-behavioural phenotypes. They show uncoordinated locomotion, occasional tremors and varying degrees of abnormal body posture immediately upon eclosion. Mutant adults spend an inordinate amount of time grooming compared to wild type. This obsessive cleaning is apparent throughout the lifetime of the animal. Mutants are capable of flying and jumping but do so only when repeatedly provoked and then only rarely. Flight in these animals is erratic. Flies recover more slowly from ether anesthesia. They show a strong temperature-dependent of behavioural defects resulting in bouts of paralysis and extreme motor uncoordination at the restrictive temperature. The behavioural phenotypes become more severe with age and some new phenotypes appear. Tremors increase dramatically such that locomotion is severely compromised in animals beyond day 50. Animals fall over and become increasingly inefficient at righting themselves. Many animals beyond day 30 show circling behaviour that varies from wide circling to circling while standing in place. A majority of animals beyond day 50 show a persistent upheld wing phenotype. Marked asymmetries appear in the animals, manifesting as one upheld wing or leg, extension of one or both back legs and more severe asymmetries in posture.

(Palladino et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Adar^5G1 has abnormal locomotor behavior | adult stage phenotype, non-enhanceable by Dcr-2^R416X

(Deng et al., 2020)

Suppressed by

Statement

Reference

Adar^5G1 has abnormal locomotor behavior | adult stage phenotype, suppressible by Scer\GAL4^ChAT.7.4/Hsap\ADARB1^UAS.cKa

(Keegan et al., 2011)

Adar^5G1 has abnormal neuroanatomy | adult stage | conditional phenotype, suppressible by Scer\GAL4^ChAT.7.4/Hsap\ADARB1^UAS.cKa

(Keegan et al., 2011)

Adar^5G1 has abnormal neuroanatomy | adult stage | conditional phenotype, suppressible | partially by Hsap\ADAR^UAS.p110/Scer\GAL4^ChAT.7.4

(Keegan et al., 2011)

NOT suppressed by

Statement

Reference

Adar^5G1 has abnormal locomotor behavior | adult stage phenotype, non-suppressible by Dcr-2^R416X

(Deng et al., 2020)

Adar^5G1 has abnormal locomotor behavior | adult stage phenotype, non-suppressible by Hsap\ADAR^UAS.p110/Scer\GAL4^ChAT.7.4

(Keegan et al., 2011)

Adar^5G1 has abnormal locomotor behavior | adult stage phenotype, non-suppressible by Hsap\ADAR^UAS.p150/Scer\GAL4^ChAT.7.4

(Keegan et al., 2011)

Adar^5G1 has abnormal locomotor behavior | adult stage phenotype, non-suppressible by Hsap\ADARB1^G.UAS/Scer\GAL4^ChAT.7.4

(Keegan et al., 2011)

Adar^5G1 has abnormal neuroanatomy | adult stage | conditional phenotype, non-suppressible by Hsap\ADAR^UAS.p150/Scer\GAL4^ChAT.7.4

(Keegan et al., 2011)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Adar^5G1 has type I bouton phenotype, non-enhanceable by Fmr1³

(Bhogal et al., 2011)

Adar^5G1 has synapse phenotype, non-enhanceable by Fmr1³

(Bhogal et al., 2011)

Adar^5G1 has neuromuscular junction phenotype, non-enhanceable by Fmr1³

(Bhogal et al., 2011)

Suppressed by

Statement

Reference

Adar^5G1 has retina | conditional phenotype, suppressible by Scer\GAL4^ChAT.7.4/Hsap\ADARB1^UAS.cKa

(Keegan et al., 2011)

Adar^5G1 has adult mushroom body | conditional phenotype, suppressible by Scer\GAL4^ChAT.7.4/Hsap\ADARB1^UAS.cKa

(Keegan et al., 2011)

Adar^5G1 has retina | conditional phenotype, suppressible | partially by Hsap\ADAR^UAS.p110/Scer\GAL4^ChAT.7.4

(Keegan et al., 2011)

Adar^5G1 has adult mushroom body | conditional phenotype, suppressible | partially by Hsap\ADAR^UAS.p110/Scer\GAL4^ChAT.7.4

(Keegan et al., 2011)

NOT suppressed by

Statement

Reference

Adar^5G1 has synapse phenotype, non-suppressible by Fmr1^+t14

(Bhogal et al., 2011)

Adar^5G1 has neuromuscular junction phenotype, non-suppressible by Fmr1^+t14

(Bhogal et al., 2011)

Adar^5G1 has type I bouton phenotype, non-suppressible by Fmr1³

(Bhogal et al., 2011)

Adar^5G1 has synapse phenotype, non-suppressible by Fmr1³

(Bhogal et al., 2011)

Adar^5G1 has neuromuscular junction phenotype, non-suppressible by Fmr1³

(Bhogal et al., 2011)

Adar^5G1 has type I bouton phenotype, non-suppressible by Fmr1^+t14

(Bhogal et al., 2011)

Adar^5G1 has retina | conditional phenotype, non-suppressible by Hsap\ADAR^UAS.p150/Scer\GAL4^ChAT.7.4

(Keegan et al., 2011)

Adar^5G1 has adult mushroom body | conditional phenotype, non-suppressible by Hsap\ADAR^UAS.p150/Scer\GAL4^ChAT.7.4

(Keegan et al., 2011)

Suppressor of

Statement

Reference

Adar^5G1 is a suppressor of type I bouton phenotype of Fmr1³

(Bhogal et al., 2011)

NOT Suppressor of

Statement

Reference

Adar^5G1 is a non-suppressor of synapse phenotype of Fmr1³

(Bhogal et al., 2011)

Adar^5G1 is a non-suppressor of neuromuscular junction phenotype of Fmr1³

(Bhogal et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Adar^5G1; Fmr1³ double mutant larvae exhibit an Adar^5G1 single mutant-like phenotype with respect to the subclasses of type 1 synaptic boutons and primary branch length.

An Adar^5G1 heterozygous background suppresses the Fmr1³ neuromuscular junction phenotype to wild-type with respect to the number of type 1 synaptic boutons.

The presence of four copies of Fmr1^+t14 does not affect the Adar^5G1 mutant phenotype with respect to type 1s and type 1b synaptic boutons, primary branch length and the number of synaptic branches.

A Adar^5G1 heterozygous background in flies expressing four copies of Fmr1^+t14 does neither suppress nor enhance the Fmr1^+t14 phenotype.

(Bhogal et al., 2011)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\ADARB1^Scer\UAS.cKa under the control of Scer\GAL4^Cha.7.4 significantly suppresses the neurodegeneration phenotype seen in the mushroom body calyces and retina of 30 day old Adar^5G1 adults.

Expression of Hsap\ADAR^{Scer\UAS.p110} under the control of Scer\GAL4^Cha.7.4 partially suppresses the neurodegeneration phenotype seen in the mushroom body calyces and retina of 30 day old Adar^5G1 adults.

Expression of Hsap\ADAR^{Scer\UAS.p150} under the control of Scer\GAL4^Cha.7.4 does not suppress the neurodegeneration phenotype seen in the mushroom body calyces and retina of 30 day old Adar^5G1 adults.

(Keegan et al., 2011)

Complementation and Rescue Data

Rescued by

Adar^5G1 is rescued by Scer\GAL4^ChAT.7.4/Adar^EA.3.4.UAS

(Deng et al., 2020)

Adar^5G1 is rescued by Scer\GAL4^βtub.PU/Adar^3.4.UAS

(Bhogal et al., 2011)

Adar^5G1 is rescued by Scer\GAL4^elav-C155/Adar^3.4.UAS

(Bhogal et al., 2011)

Adar^5G1 is rescued by Scer\GAL4^scrt-11-6/Adar^3.4.UAS

(Bhogal et al., 2011)

Adar^5G1 is rescued by Adar^3.4.S.UAS/Scer\GAL4^ChAT.7.4

(Keegan et al., 2011)

Adar^5G1 is rescued by Adar^3.4.S.UAS/Scer\GAL4^Toll-6-D42

(Keegan et al., 2011)

Adar^5G1 is rescued by Scer\GAL4^RapGAP1-OK6/Adar^3.4.S.UAS

(Keegan et al., 2011)

Adar^5G1 is rescued by Scer\GAL4^ChAT.7.4/Adar^3.4.UAS

(Keegan et al., 2011)

Adar^5G1 is rescued by Adar^3.4.S.UAS/Scer\GAL4^ChAT.7.4

(Keegan et al., 2005)

Partially rescued by

Adar^5G1 is partially rescued by Scer\GAL4^ChAT.7.4/Adar^3.4.UAS

(Deng et al., 2020)

Adar^5G1 is partially rescued by Scer\GAL4^VGlut1-OK371/Adar^3.4.S.UAS

(Keegan et al., 2011)

Not rescued by

Adar^5G1 is not rescued by Scer\GAL4^Mhc.PU/Adar^3.4.UAS

(Bhogal et al., 2011)

Adar^5G1 is not rescued by Scer\GAL4^G14/Adar^3.4.UAS

(Bhogal et al., 2011)

Adar^5G1 is not rescued by Scer\GAL4^βtub.PU/Adar^EA.3.4.UAS

(Bhogal et al., 2011)

Adar^5G1 is not rescued by Adar^3.4.S.UAS/Scer\GAL4^Ddc.PL

(Keegan et al., 2011)

Adar^5G1 is not rescued by Adar^3.4.S.UAS/Scer\GAL4^Tdc2.PC

(Keegan et al., 2011)

Adar^5G1 is not rescued by Adar^3.4.S.UAS/Scer\GAL4^shot-OK307

(Keegan et al., 2011)

Adar^5G1 is not rescued by Scer\GAL4^ChAT.7.4/Adar^EA.3.4.UAS

(Keegan et al., 2011)

Comments

Ubiquitous expression of Adar^3.4.Scer\UAS under the control of Scer\GAL4^βtub.PU completely rescues the neuromuscular junction phenotype observed in Adar^5G1 larvae with respect to synaptic bouton number and branching.

(Bhogal et al., 2011)

Expression of Adar^3.4.Scer\UAS under the control of Scer\GAL4^Cha.7.4 rescues the locomotor defects seen in Adar^5G1 adults.

Expression of Adar^3.4.Scer\UAS under the control of Scer\GAL4^Cha.7.4 fails to rescue the locomotor defects seen in Adar^5G1 adults.

Expression of Adar^{3.4.S.Scer\UAS} under the control of any one of Scer\GAL4^Cha.7.4, Scer\GAL4^D42 or Scer\GAL4^Rapgap1-OK6 rescues the locomotor defects seen in Adar^5G1 adults.

Expression of Adar^{3.4.S.Scer\UAS} under the control of Scer\GAL4^VGlut-OK371 partially rescues the locomotor defects seen in Adar^5G1 adults.

Expression of Adar^{3.4.S.Scer\UAS} under the control of any one of Scer\GAL4^Ddc.PL, Scer\GAL4^Tdc2.PC or Scer\GAL4^OK307 does not rescue the locomotor defects seen in Adar^5G1 adults.

Expression of Adar^3.4.Scer\UAS under the control of Scer\GAL4^Cha.7.4 significantly rescues the neurodegeneration phenotype seen in the mushroom body calyces and retina of 30 day old Adar^5G1 adults.

(Keegan et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Adar^5G1

(Deng et al., 2020, Sapiro et al., 2020, Buchumenski et al., 2017, Li et al., 2014, Savva et al., 2012, Bhogal et al., 2011, Keegan et al., 2011)

Adar^5g1

(Rieder et al., 2013, Jepson et al., 2011)

adar^5G1

(Chawla and Sokol, 2014)

dADAR^5G1

(Ingleby et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (15)

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