FlyBase Allele Report: Dmel\Adar[3.4.S.UAS]

General Information

Symbol

Dmel\Adar^3.4.S.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0189999

Feature type

allele

Associated gene

Dmel\Adar

Associated Insertion(s)

Carried in Construct

P{UAS-Adar.3.4.S}

Also Known As

UAS-Adar 3/4 S

Key Links

Transgenic product class

editing_variant

(Keegan et al., 2005)

silent_mutation

(Keegan et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

editing_variant

(Keegan et al., 2005)

silent_mutation

(Keegan et al., 2005)

Mutagen

in vitro construct

(Keegan et al., 2005)

Progenitor genotype

Adar^3.4.UAS

(Keegan et al., 2005)

Carried in construct

P{UAS-Adar.3.4.S}

Cytology

Description

UASt regulatory sequences drive expression of cDNA encoding the Adar 3/4 isoform that has been mutated to produce a non-editable mRNA; the codon encoding the Ser residue in exon 7 close to the active site which is normally edited by the Adar protein has been mutated to another Ser codon that does not have an A at the first position (codon is changed from AGT to TCT). The coding region is tagged with both Tag:FLAG and Tag:polyHis.

(Keegan et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tagged with

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | larval stage, with Scer\GAL4^eve.RRa

(Li et al., 2014)

lethal, with Adar^1F4, Scer\GAL4^Act5C.PI

(Keegan et al., 2005)

lethal, with Adar^5G1, Scer\GAL4^Mef2.PR

(Keegan et al., 2005)

lethal, with Scer\GAL4^Mef2.PR

(Keegan et al., 2005)

lethal - all die before end of P-stage, with Adar^5G1, Scer\GAL4^Act5C.PI

(Li et al., 2014)

lethal - all die before end of P-stage, with Scer\GAL4^Act5C.PI

(Li et al., 2014)

viable, with Adar^5G1, Scer\GAL4^ChAT.7.4

(Keegan et al., 2005)

Phenotype Manifest In

larval DA1 motor neuron | larval stage, with Scer\GAL4^eve.RRa

(Li et al., 2014)

Detailed Description

Statement

Reference

Expression of Adar^{3.4.S.Scer\UAS} under the control of Scer\GAL4^Act5C.PI leads to lethality in larval and pupal stages. This lethality is not rescued in males hemizygous for the Adar^5G1 mutation.

Expression of Adar^{3.4.S.Scer\UAS} under the control of Scer\GAL4^eve.RRa leads to significantly reduced firing activity of larval aCC neurons.

(Li et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Adar^3.4.S.UAS, Scer\GAL4^Act5C.PI has lethal - all die before end of P-stage phenotype, suppressible | partially by Df(3L)Rdl-2/+

(Li et al., 2014)