FlyBase Allele Report: Dmel\Dcr-2[R416X]

General Information

Symbol

Dmel\Dcr-2^R416X

Species

D. melanogaster

Name

FlyBase ID

FBal0156732

Feature type

allele

Associated gene

Dmel\Dcr-2

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Lee et al., 2004)

Progenitor genotype

Cytology

Description

Amino acid replacement: R416term.

(Lee et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:17,579,505..17,579,505 [-]

Nucleotide change:

C17579505T

Amino acid change:

R416term | Dcr-2-PA; R416term | Dcr-2-PB

Reported amino acid change:

R416term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Lee et al., 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | conditional

(Mueller et al., 2010)

abnormal immune response | recessive

(Kemp et al., 2013)

abnormal stress response | recessive

(Kemp et al., 2013)

suppressor of variegation (with Dcr-2^L811fsX)

(Fagegaltier et al., 2009)

suppressor of variegation | dominant

(Fagegaltier et al., 2009)

suppressor of variegation | dominant, with P{hsp26-pt-T}8-M76

(Wang et al., 2014)

suppressor of variegation | dominant, with P{hsp26-pt-T}39C-66

(Wang et al., 2014)

suppressor of variegation | dominant, with P{w⁺(-1360)}1250

(Wang et al., 2014)

suppressor of variegation | dominant, with P{w⁺(FRT.attP.1360)}1250

(Wang et al., 2014)

suppressor of variegation | partially, with P{T1(-FRT)}T190-177

(Haynes et al., 2006)

suppressor of variegation | partially, with P{T1}T190-177

(Haynes et al., 2006)

wild-type

(Lee et al., 2004)

Phenotype Manifest In

Detailed Description

Statement

Reference

Injection of Dcr-2^R416X mutant flies with Invertebrate Iridescent Virus type 6 (IIV-6) leads to a more rapid and intense appearance of blue colour, which is characteristic of the accumulation of iridescent viral particles, than in wild-type control. Dcr-2^R416X mutant flies are significantly more susceptible to IIV-6 infection than are wild-type. A fraction of Dcr-2^R416X flies injected with buffer also die in the course of the experiment, indicating an increased sensitivity to stress associated with the mutation. The decreased survival time correlated with a 20-fold increased viral load in Dcr-2^R416X mutant flies at 10 d post-infection.

(Kemp et al., 2013)

Dcr-2^R416X mutant flies show severely compromised survival when infected with vesicular stomatitis virus, and die within 12 days of infection. Viral titres are significantly higher compared to controls.

(Mueller et al., 2010)

Dcr-2^R416X/+ significantly suppresses the position effect variegation of the Sb¹ allele that is seen in the T(2;3)Sb^V chromosome.

Dcr-2^L811fsX/Dcr-2^R416X suppresses the position effect variegation of the w gene that is seen in the In(1)w^m4 chromosome.

(Fagegaltier et al., 2009)

flies show a slight disruption of the heterochromatic silencing of the P{T1} reporter in line P{T1}T190-177 and of the P{T1(-FRT)} reporter in line P{T1(-FRT)}T190-177.

(Haynes et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Dcr-2^R416X is a non-enhancer of abnormal immune response | adult stage phenotype of Veneno^Doc

(Brosh et al., 2022)

Dcr-2^R416X is a non-enhancer of abnormal locomotor behavior | adult stage phenotype of Adar^5G1

(Deng et al., 2020)

Dcr-2^R416X is a non-enhancer of abnormal locomotor behavior | adult stage phenotype of Adar^E374A

(Deng et al., 2020)

NOT Suppressor of

Statement

Reference

Dcr-2^R416X is a non-suppressor of abnormal immune response | adult stage phenotype of Veneno^Doc

(Brosh et al., 2022)

Dcr-2^R416X is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Adar^5G1

(Deng et al., 2020)

Dcr-2^R416X is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Adar^E374A

(Deng et al., 2020)

Other

Statement

Reference

Dcr-1^Q1147X/Dcr-1[+], Dcr-2^R416X has female fertile phenotype

(Kalidas et al., 2008)

Phenotype Manifest In

Suppressor of

Statement

Reference

Dcr-2^R416X is a suppressor of eye phenotype of w^RNAi.GMR

(Lee et al., 2004)

Dcr-2^R416X is a suppressor of pigment cell phenotype of w^RNAi.GMR

(Lee et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Dcr-2^R416X ; Dcr-1^Q1147X/+ females have normal ovaries and normal fertility.

(Kalidas et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Dcr-2^R416X

(Brosh et al., 2022, Deng et al., 2020, Lee et al., 2018, Chtarbanova et al., 2014, Carré et al., 2013, Kemp et al., 2013, Mueller et al., 2010, Deddouche et al., 2008)

Dicer-2^R416X

(Harsh et al., 2018)

dcr-2^R416X

(Vedanayagam et al., 2023, Wang et al., 2014, Lim et al., 2013, Kalidas et al., 2008, Haynes et al., 2006)

dcr2^R416X

(Fagegaltier et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (17)

Report Sections

Open Close