FlyBase Allele Report: Dmel\Dcr-2[L811fsX]

General Information

Symbol

Dmel\Dcr-2^L811fsX

Species

D. melanogaster

Name

FlyBase ID

FBal0156733

Feature type

allele

Associated gene

Dmel\Dcr-2

Associated Insertion(s)

Carried in Construct

Also Known As

dcr2^L811fsX, dicer-2^L811fsX, Dcr-2^L811X, dicer-2^null

Key Links

Genomic Maps

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Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Grimaud et al., 2006)

Mutagen

ethyl methanesulfonate

(Lee et al., 2004)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

complex_substitution

2R:17,578,131..17,578,133 [-]

Comment:

Leu 811 is replaced by FGIRSLCWIVAARRTPTWX.

(Lee et al., 2004)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

The Leucine at residue 811 is replaced by FGIRSLCWIVAARRTPTWX.

(Lee et al., 2004)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

nucleus | male (with Df(2R)BSC45)

(Wen et al., 2015)

spermatid (with Df(2R)BSC45)

(Wen et al., 2015)

spermatozoon (with Df(2R)BSC45)

(Wen et al., 2015)

Detailed Description

Statement

Reference

Dcr-2^L811fsX homozygotes do not show significant differences in viral load in the head upon infection with ZIKV, as compared to infected controls.

(Liu et al., 2018)

The fertility of Dcr-2^L811fsX/Df(2R)BSC45 mutant males is severely reduced compared to wild-type controls (female fertility is not affected) due decreased number of mature sperm in their seminal vesicle. The spermatid nuclear alignment as well as the alignment of the investment cones in thee testes is disrupted.

(Wen et al., 2015)

The survival rate of DIP1^EY02625 mutants after DCV infection is 17% compared to controls. Median survival is 5-6 days.

(Zhang et al., 2015)

Compared with DCV-infected wild-type flies, Dcr-2^L811fsX homozygotes have a much higher viral load.

(Lim et al., 2014)

Dcr-2^L811fsX/Df(2R)BSC45 flies injected with Invertebrate Iridescent Virus type 6 (IIV-6) die earlier than IIV-6 infected wild-type controls. A fraction of Dcr-2^L811fsX/Df(2R)BSC45 flies injected with buffer also die in the course of the experiment, indicating an increased sensitivity to stress associated with the mutation.

(Kemp et al., 2013)

Dcr-2^L811fsX mutant flies are more susceptible to non-virulent Flock house virus (FHV) mutant infection than wild-type. The FHV mutant that lacks viral protein FHV\B2, a known RNAi suppressor, is not pathogenic in wild-type flies, but Dcr-2^L811fsX mutants show decreased survival after infection. Unlike the case in wild-type flies, the FHV FHV\B2 mutant replicates to high levels in Dcr-2^L811fsX mutants.

(Han et al., 2011)

More than 60% of cells in anaphase mutant wing discs contain lagging chromosomes.

(Pek and Kai, 2011)

Dcr-2^L811fsX/+ significantly suppresses the position effect variegation of the Sb¹ allele that is seen in the T(2;3)Sb^V chromosome.

Dcr-2^L811fsX/Dcr-2^R416X suppresses the position effect variegation of the w gene that is seen in the In(1)w^m4 chromosome.

(Fagegaltier et al., 2009)

Homozygous projection neurons show normal dendrite and axon targeting.

(Berdnik et al., 2008)

Dcr-2^L811fsX mutant germline stem cells (GSCs) do not exhibit any maintenance defects when clones are induced either during larval/pupal stages or during adult stages.

(Shcherbata et al., 2007)

Mutant flies are more susceptible to infection with RNA viruses (Flock House virus, Drosophila C virus or Sindbis virus) than control flies.

(Galiana-Arnoux et al., 2006)

Embryos derived from Dcr-2^L811fsX mothers exhibit normal cellularization and net lipid-droplet transport.

(Meyer et al., 2006)

Dcr-2^L811fsX homozygous flies fail to produce siRNA from a transfected construct.

(Vagin et al., 2006)

Dcr-2^L811fsX flies show increased mortality and an increased level of viral accumulation following infection with both Flock house virus (FHV) and cricket paralysis virus (CrPV).

(Wang et al., 2006)

Mutant flies are hypersensitive to infection with Cricket Paralysis virus (CrPV); the median survival after inoculation with 350 TCID₅₀ of CrPV is reduced compared to controls.

(van Rij et al., 2006)

When Dcr-2^L811fsX germ-line clones are made a normal progression of egg chambers is seen.

(Hatfield et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Dcr-2[+]/Dcr-2^L811fsX is a non-enhancer of visible phenotype of Scer\GAL4^da.G32, Zzzz\CAG^{99.UAS.Tag:MYC,Tag:FLAG}

(Lawlor et al., 2012)

Dcr-2[+]/Dcr-2^L811fsX is a non-enhancer of visible phenotype of Scer\GAL4^da.G32, Zzzz\CTG^{rCUG.99.UAS.Tag:MYC,Tag:FLAG}

(Lawlor et al., 2012)

Dcr-2^L811fsX, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-enhancer of increased cell death phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Bilen et al., 2006)

Suppressor of

Statement

Reference

Dcr-2[+]/Dcr-2^L811fsX is a suppressor | partially of partially lethal - majority live phenotype of ct^A, lncRNA:flam^A

(Guida et al., 2016)

Dcr-2^L811fsX is a suppressor of visible phenotype of w^dsRNA.GMR/w^dsRNA.GMR

(Fukunaga et al., 2012)

Dcr-2[+]/Dcr-2^L811fsX is a suppressor | partially of visible phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{99.UAS.Tag:MYC,Tag:FLAG}, Zzzz\CTG^{rCUG.99.UAS.Tag:MYC,Tag:FLAG}

(Lawlor et al., 2011)

Dcr-2^L811fsX is a suppressor of increased mortality during development phenotype of Zzzz\CTG^{130.UAS.DsRed2}/Disc\RFP^{(CTG)130.Scer\UAS}, Zzzz\CAG^{100.UAS.DsRed2}/Disc\RFP^{CAG100.DsRed2.Scer\UAS}, Scer\GAL4^how-24B

(Yu et al., 2011)

Dcr-2^L811fsX is a suppressor of increased mortality during development phenotype of Zzzz\CTG^{250.UAS.DsRed2}/Disc\RFP^{(CTG)250.Scer\UAS}, Disc\RFP^{CAG250.DsRed2.Scer\UAS}/Zzzz\CAG^{250.UAS.DsRed2}, Scer\GAL4^GMR.PF

(Yu et al., 2011)

Dcr-2^L811fsX is a suppressor of abnormal eye color phenotype of w^IR.GMR

(Lim et al., 2008)

Dcr-2^L811fsX is a suppressor | somatic clone of abnormal eye color phenotype of w^IR.GMR

(Zhou et al., 2008)

Dcr-2^L811fsX/Dcr-2^L811fsX is a suppressor of abnormal eye color phenotype of w^dsRNA.GMR/w^dsRNA.GMR

(Pelisson et al., 2007)

Dcr-2[+]/Dcr-2^L811fsX is a suppressor | partially of increased cell death phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Diap1^dsRNA.UAS

(Meyer et al., 2006)

NOT Suppressor of

Statement

Reference

Dcr-2[+]/Dcr-2^L811fsX is a non-suppressor of visible phenotype of Scer\GAL4^da.G32, Zzzz\CAG^{99.UAS.Tag:MYC,Tag:FLAG}

(Lawlor et al., 2012)

Dcr-2[+]/Dcr-2^L811fsX is a non-suppressor of visible phenotype of Scer\GAL4^da.G32, Zzzz\CTG^{rCUG.99.UAS.Tag:MYC,Tag:FLAG}

(Lawlor et al., 2012)

Dcr-2^L811fsX, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-suppressor of increased cell death phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Bilen et al., 2006)

Other

Statement

Reference

Dcr-1^Q1147X, Dcr-2^L811fsX has decreased cell number | somatic clone | larval stage phenotype

(Shcherbata et al., 2007)

Dcr-1^Q1147X, Dcr-2^L811fsX has decreased cell number | somatic clone | pupal stage phenotype

(Shcherbata et al., 2007)

Phenotype Manifest In

Suppressed by

Statement

Reference

Dcr-2^L811fsX, w^IR.GMR has eye phenotype, suppressible | partially by Dcr-2^E1617K.t7.2

(Lee et al., 2004)

Dcr-2^L811fsX, w^IR.GMR has pigment cell phenotype, suppressible | partially by Dcr-2^E1617K.t7.2

(Lee et al., 2004)

Dcr-2^L811fsX, w^IR.GMR has eye phenotype, suppressible by Dcr-2^E1210V.t7.2

(Lee et al., 2004)

Dcr-2^L811fsX, w^IR.GMR has pigment cell phenotype, suppressible by Dcr-2^E1210V.t7.2

(Lee et al., 2004)

Dcr-2^L811fsX, w^IR.GMR has eye phenotype, suppressible | partially by Dcr-2^E1371K.t7.2

(Lee et al., 2004)

Dcr-2^L811fsX, w^IR.GMR has pigment cell phenotype, suppressible | partially by Dcr-2^E1371K.t7.2

(Lee et al., 2004)

NOT suppressed by

Statement

Reference

Dcr-2^L811fsX, w^IR.GMR has eye phenotype, non-suppressible by Dcr-2^{E1371K.E1617K.t7.2}

(Lee et al., 2004)

Dcr-2^L811fsX, w^IR.GMR has pigment cell phenotype, non-suppressible by Dcr-2^{E1371K.E1617K.t7.2}

(Lee et al., 2004)

Enhancer of

Statement

Reference

Dcr-2[+]/Dcr-2^L811fsX is an enhancer of phenotype of Zzzz\rep^{Scer\UAS.T:Avic\GFP}/Sindbis\rep^UAS.GFP

(Avadhanula et al., 2009)

NOT Enhancer of

Statement

Reference

Dcr-2[+]/Dcr-2^L811fsX is a non-enhancer of tergite phenotype of Scer\GAL4^da.G32, Zzzz\CAG^{99.UAS.Tag:MYC,Tag:FLAG}

(Lawlor et al., 2012)

Dcr-2[+]/Dcr-2^L811fsX is a non-enhancer of tergite phenotype of Scer\GAL4^da.G32, Zzzz\CTG^{rCUG.99.UAS.Tag:MYC,Tag:FLAG}

(Lawlor et al., 2012)

Dcr-2^L811fsX, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Bilen et al., 2006)

Dcr-2^L811fsX, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-enhancer of retina phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Bilen et al., 2006)

Suppressor of

Statement

Reference

Dcr-2^L811fsX is a suppressor of eye phenotype of w^dsRNA.GMR/w^dsRNA.GMR

(Fukunaga et al., 2012)

Dcr-2[+]/Dcr-2^L811fsX is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{99.UAS.Tag:MYC,Tag:FLAG}, Zzzz\CTG^{rCUG.99.UAS.Tag:MYC,Tag:FLAG}

(Lawlor et al., 2011)

Dcr-2^L811fsX/Dcr-2^L811fsX is a suppressor of eye phenotype of w^dsRNA.GMR/w^dsRNA.GMR

(Pelisson et al., 2007)

Dcr-2^L811fsX/Dcr-2^L811fsX is a suppressor of wing phenotype of sd^Fab-X

(Grimaud et al., 2006)

Dcr-2[+]/Dcr-2^L811fsX is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Diap1^dsRNA.UAS

(Meyer et al., 2006)

Dcr-2^L811fsX is a suppressor of eye phenotype of w^IR.GMR

(Lee et al., 2004)

Dcr-2^L811fsX is a suppressor of pigment cell phenotype of w^IR.GMR

(Lee et al., 2004)

NOT Suppressor of

Statement

Reference

Dcr-2[+]/Dcr-2^L811fsX is a non-suppressor of tergite phenotype of Scer\GAL4^da.G32, Zzzz\CAG^{99.UAS.Tag:MYC,Tag:FLAG}

(Lawlor et al., 2012)

Dcr-2[+]/Dcr-2^L811fsX is a non-suppressor of tergite phenotype of Scer\GAL4^da.G32, Zzzz\CTG^{rCUG.99.UAS.Tag:MYC,Tag:FLAG}

(Lawlor et al., 2012)

Dcr-2^L811fsX, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Bilen et al., 2006)

Dcr-2^L811fsX, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF is a non-suppressor of retina phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Bilen et al., 2006)

Other

Statement

Reference

Dcr-1^Q1147X, Dcr-2^L811fsX has female germline stem cell | somatic clone | larval stage phenotype

(Shcherbata et al., 2007)

Dcr-1^Q1147X, Dcr-2^L811fsX has female germline stem cell | somatic clone | pupal stage phenotype

(Shcherbata et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Dcr-2^L811fsX heterozygosity partially suppresses the mortality of flam^A, ct^A double homozygotes.

(Guida et al., 2016)

The eyes of w^dsRNA.GMR transgenic female flies homozygous for Dcr-2^L811fsX are similar to that of wild-type.

(Fukunaga et al., 2012)

A Dcr-2^L811fsX homozygous mutant background suppresses the developmental lethality associated with expression of Disc\RFP^{(CTG)130.Scer\UAS} and Disc\RFP^{CAG100.DsRed2.Scer\UAS} in the developing muscle (under the control of Scer\GAL4^how-24B).

A Dcr-2^L811fsX homozygous mutant background suppresses the organismal lethality associated with 30 minute heat-shock induced expression of Disc\RFP^{CAG250.DsRed2.Scer\UAS} and Disc\RFP^{(CTG)250.Scer\UAS} (under the control of Scer\GAL4^hs.PB).

(Yu et al., 2011)

w^IR.GMR eyes that are also homozygous for Dcr-2^L811fsX show strong suppression of the pale eye colour phenotype.

(Lim et al., 2008)

Flies carrying w^IR.GMR in which the eyes are homozygous for Dcr-2^L811fsX have dark red eyes.

(Zhou et al., 2008)

Flies carrying one copy of w^dsRNA.GMR in a homozygous Dcr-2^L811fsX background have red eyes.

(Pelisson et al., 2007)

Dcr-1^Q1147X Dcr-2^L811fsX double mutants show strong germline stem cell maintenance (GSC) defects when GSC clones are induced during late larval/early pupal stages.

(Shcherbata et al., 2007)

Eye degeneration due to expression of Hsap\MJD^{tr.Q78.Scer\UAS.T:Ivir\HA1} in the eye (under the control of Scer\GAL4^GMR.PF) is unaffected by a Dcr-2^L811fsX background.

(Bilen et al., 2006)

The wing phenotype of sd^Fab-X flies is suppressed in sd^Fab-X;Dcr-2^L811fsX flies.

(Grimaud et al., 2006)

A Dcr-2^L811fsX/+ background partially suppresses the small eye phenotype that is seen when th^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF.

Dcr-2^L811fsX; Dcr-1^Q1147X double mutants show no segment polarity defects.

(Meyer et al., 2006)

Xenogenetic Interactions

Statement

Reference

Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} expression using Scer\GAL4^da.G32 in the presence of heterozygous Dcr-2^L811fsX does not give significantly different phenotype severity compared to Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} expression in a wild-type Dcr-2 background.

Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} expression using Scer\GAL4^da.G32 in the presence of heterozygous Dcr-2^L811fsX does not give significantly different phenotype severity compared to Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} expression in a wild-type Dcr-2 background.

(Lawlor et al., 2012)

Heterozygous Dcr-2^L811fsX suppresses the eye-phenotype resulting from the co-expression of Zzzz\CAG^{99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} and Zzzz\CAG^{rCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF. In addition, the overall disruption observed in patterning of the internal structure of the eye is significantly reduced in the presence of heterozygous Dcr-2^L811fsX as revealed by sections through adult eyes.

(Lawlor et al., 2011)

Heterozygosity for Dcr-2^L811fsX in a Scer\GAL4^Act5C.PI Zzzz\rep^{Scer\UAS.T:Avic\GFP} background results in increased Sindbis viral replication.

(Avadhanula et al., 2009)

Complementation and Rescue Data

Rescued by

Dcr-2^L811fsX/Df(2R)BSC45 is rescued by Dcr-2^+tKa

(Wen et al., 2015)

Dcr-2^L811fsX/Df(2R)BSC45 is rescued by Dcr-2^+tKa

(Kemp et al., 2013)

Comments

The severely reduced fertility of Dcr-2^L811fsX/Df(2R)BSC45 mutant males along with their sperm deficiency is rescued by combination with Dcr-2^+tKa.

(Wen et al., 2015)

Dcr-2^+tKa rescues the increased susceptibility of Dcr-2^L811fsX/Df(2R)BSC45 mutant flies to IIV-6 infection.

(Kemp et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

33053

y^d2 w¹¹¹⁸ P{ey-FLP.N}2; P{neoFRT}42D Dcr-2^L811fsX/CyO; P{Dcr-2^+t7.2}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (19)

Reported As

Symbol Synonym

Dcr-2^L811FSX

(Deddouche et al., 2008)

Dcr-2^L811FXS

(Avadhanula et al., 2009)

Dcr-2^L811Fsx

(Mueller et al., 2010, Czech et al., 2008)

Dcr-2^L811X

(Hatfield et al., 2005)

Dcr-2^L811fsx

(Eyk et al., 2019, Habayeb et al., 2009)

Dcr2^KO

(Chow et al., 2017)

Dcr2^L811fsX

(Coll et al., 2018, Chow et al., 2017, Lamiable et al., 2016)

Dicer-2^L811fsX

(Berdnik et al., 2008)

dcr-2^L811Ex

(Ashraf et al., 2006)

dcr-2^L811X

(Shcherbata et al., 2007)

dcr-2^L811fs

(Martin et al., 2009)

dcr2^L811fsX

(Dufourt et al., 2011, Lawlor et al., 2011, Okamura et al., 2011, Yu et al., 2011, Fagegaltier et al., 2009)

dicer-2^L811fsX

(Harris et al., 2011)

dicer-2^L811fsx

(Kandasamy et al., 2017, Kandasamy and Fukunaga, 2016)

dicer-2^null

(Kandasamy et al., 2017, Kandasamy and Fukunaga, 2016)

dicer2^L811fsX

(Martins et al., 2019)

Name Synonyms

Suppressor(GMR-wIR)A293

(Lee et al., 2004)

Secondary FlyBase IDs

References (71)

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