FB2022_03, released June 9, 2022

Allele: Dmel\Dcr-2L811fsX

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General Information
Symbol
Dmel\Dcr-2L811fsX
Species
D. melanogaster
Name
FlyBase ID
FBal0156733
Feature type
allele
Associated gene
Dmel\Dcr-2
Associated Insertion(s)
Carried in Construct
Also Known As
dcr2L811fsX, dicer-2L811fsX, Dcr-2L811X, dicer-2null
Key Links
Genomic Maps
Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Grimaud et al., 2006)
Mutagen
ethyl methanesulfonate
(Lee et al., 2004)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
delins
2R:17,578,131..17,578,133 [-]
Comment:

Leu 811 is replaced by FGIRSLCWIVAARRTPTWX.

(Lee et al., 2004)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Cytology
      Nature of the lesion
      Statement
      Reference

      The Leucine at residue 811 is replaced by FGIRSLCWIVAARRTPTWX.

      (Lee et al., 2004)
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Dcr-2L811fsX adults systemically infected, but not orally infected, with Drosophila C Virus (DCV) show increased viral load compared to controls; oral infection with Sindbis virus (SINV) does not lead to increased viral load compared to controls.

      (Segrist et al., 2021)

      Dcr-2L811fsX homozygotes do not show significant differences in viral load in the head upon infection with ZIKV, as compared to infected controls.

      (Liu et al., 2018)

      The fertility of Dcr-2L811fsX/Df(2R)BSC45 mutant males is severely reduced compared to wild-type controls (female fertility is not affected) due decreased number of mature sperm in their seminal vesicle. The spermatid nuclear alignment as well as the alignment of the investment cones in thee testes is disrupted.

      (Wen et al., 2015)

      The survival rate of DIP1EY02625 mutants after DCV infection is 17% compared to controls. Median survival is 5-6 days.

      (Zhang et al., 2015)

      Compared with DCV-infected wild-type flies, Dcr-2L811fsX homozygotes have a much higher viral load.

      (Lim et al., 2014)

      Dcr-2L811fsX/Df(2R)BSC45 flies injected with Invertebrate Iridescent Virus type 6 (IIV-6) die earlier than IIV-6 infected wild-type controls. A fraction of Dcr-2L811fsX/Df(2R)BSC45 flies injected with buffer also die in the course of the experiment, indicating an increased sensitivity to stress associated with the mutation.

      (Kemp et al., 2013)

      Dcr-2L811fsX mutant flies are more susceptible to non-virulent Flock house virus (FHV) mutant infection than wild-type. The FHV mutant that lacks viral protein FHV\B2, a known RNAi suppressor, is not pathogenic in wild-type flies, but Dcr-2L811fsX mutants show decreased survival after infection. Unlike the case in wild-type flies, the FHV FHV\B2 mutant replicates to high levels in Dcr-2L811fsX mutants.

      (Han et al., 2011)

      More than 60% of cells in anaphase mutant wing discs contain lagging chromosomes.

      (Pek and Kai, 2011)

      Dcr-2L811fsX/+ significantly suppresses the position effect variegation of the Sb1 allele that is seen in the T(2;3)SbV chromosome.

      Dcr-2L811fsX/Dcr-2R416X suppresses the position effect variegation of the w gene that is seen in the In(1)wm4 chromosome.

      (Fagegaltier et al., 2009)

      Homozygous projection neurons show normal dendrite and axon targeting.

      (Berdnik et al., 2008)

      Dcr-2L811fsX mutant germline stem cells (GSCs) do not exhibit any maintenance defects when clones are induced either during larval/pupal stages or during adult stages.

      (Shcherbata et al., 2007)

      Mutant flies are more susceptible to infection with RNA viruses (Flock House virus, Drosophila C virus or Sindbis virus) than control flies.

      (Galiana-Arnoux et al., 2006)

      Embryos derived from Dcr-2L811fsX mothers exhibit normal cellularization and net lipid-droplet transport.

      (Meyer et al., 2006)

      Dcr-2L811fsX homozygous flies fail to produce siRNA from a transfected construct.

      (Vagin et al., 2006)

      Dcr-2L811fsX flies show increased mortality and an increased level of viral accumulation following infection with both Flock house virus (FHV) and cricket paralysis virus (CrPV).

      (Wang et al., 2006)

      Mutant flies are hypersensitive to infection with Cricket Paralysis virus (CrPV); the median survival after inoculation with 350 TCID50 of CrPV is reduced compared to controls.

      (van Rij et al., 2006)

      When Dcr-2L811fsX germ-line clones are made a normal progression of egg chambers is seen.

      (Hatfield et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Other
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      Dcr-2L811fsX, wIR.GMR has eye phenotype, suppressible | partially by Dcr-2E1617K.t7.2

      (Lee et al., 2004)

      Dcr-2L811fsX, wIR.GMR has pigment cell phenotype, suppressible | partially by Dcr-2E1617K.t7.2

      (Lee et al., 2004)

      Dcr-2L811fsX, wIR.GMR has eye phenotype, suppressible by Dcr-2E1210V.t7.2

      (Lee et al., 2004)

      Dcr-2L811fsX, wIR.GMR has pigment cell phenotype, suppressible by Dcr-2E1210V.t7.2

      (Lee et al., 2004)

      Dcr-2L811fsX, wIR.GMR has eye phenotype, suppressible | partially by Dcr-2E1371K.t7.2

      (Lee et al., 2004)

      Dcr-2L811fsX, wIR.GMR has pigment cell phenotype, suppressible | partially by Dcr-2E1371K.t7.2

      (Lee et al., 2004)
      NOT suppressed by
      Statement
      Reference

      Dcr-2L811fsX, wIR.GMR has eye phenotype, non-suppressible by Dcr-2E1371K.E1617K.t7.2

      (Lee et al., 2004)

      Dcr-2L811fsX, wIR.GMR has pigment cell phenotype, non-suppressible by Dcr-2E1371K.E1617K.t7.2

      (Lee et al., 2004)
      Enhancer of
      Statement
      Reference

      Dcr-2[+]/Dcr-2L811fsX is an enhancer of phenotype of Zzzz\repScer\UAS.T:Avic\GFP/Sindbis\repUAS.GFP

      (Avadhanula et al., 2009)
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Dcr-2L811fsX heterozygosity partially suppresses the mortality of flamA, ctA double homozygotes.

      (Guida et al., 2016)

      The eyes of wdsRNA.GMR transgenic female flies homozygous for Dcr-2L811fsX are similar to that of wild-type.

      (Fukunaga et al., 2012)

      A Dcr-2L811fsX homozygous mutant background suppresses the developmental lethality associated with expression of Disc\RFP(CTG)130.Scer\UAS and Disc\RFPCAG100.DsRed2.Scer\UAS in the developing muscle (under the control of Scer\GAL4how-24B).

      A Dcr-2L811fsX homozygous mutant background suppresses the organismal lethality associated with 30 minute heat-shock induced expression of Disc\RFPCAG250.DsRed2.Scer\UAS and Disc\RFP(CTG)250.Scer\UAS (under the control of Scer\GAL4hs.PB).

      (Yu et al., 2011)

      wIR.GMR eyes that are also homozygous for Dcr-2L811fsX show strong suppression of the pale eye colour phenotype.

      (Lim et al., 2008)

      Flies carrying wIR.GMR in which the eyes are homozygous for Dcr-2L811fsX have dark red eyes.

      (Zhou et al., 2008)

      Flies carrying one copy of wdsRNA.GMR in a homozygous Dcr-2L811fsX background have red eyes.

      (Pelisson et al., 2007)

      Dcr-1Q1147X Dcr-2L811fsX double mutants show strong germline stem cell maintenance (GSC) defects when GSC clones are induced during late larval/early pupal stages.

      (Shcherbata et al., 2007)

      Eye degeneration due to expression of Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 in the eye (under the control of Scer\GAL4GMR.PF) is unaffected by a Dcr-2L811fsX background.

      (Bilen et al., 2006)

      The wing phenotype of sdFab-X flies is suppressed in sdFab-X;Dcr-2L811fsX flies.

      (Grimaud et al., 2006)

      A Dcr-2L811fsX/+ background partially suppresses the small eye phenotype that is seen when thdsRNA.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF.

      Dcr-2L811fsX; Dcr-1Q1147X double mutants show no segment polarity defects.

      (Meyer et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference

      Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG expression using Scer\GAL4da.G32 in the presence of heterozygous Dcr-2L811fsX does not give significantly different phenotype severity compared to Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG expression in a wild-type Dcr-2 background.

      Zzzz\CAGrCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG expression using Scer\GAL4da.G32 in the presence of heterozygous Dcr-2L811fsX does not give significantly different phenotype severity compared to Zzzz\CAGrCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG expression in a wild-type Dcr-2 background.

      (Lawlor et al., 2012)

      Heterozygous Dcr-2L811fsX suppresses the eye-phenotype resulting from the co-expression of Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG and Zzzz\CAGrCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4GMR.PF. In addition, the overall disruption observed in patterning of the internal structure of the eye is significantly reduced in the presence of heterozygous Dcr-2L811fsX as revealed by sections through adult eyes.

      (Lawlor et al., 2011)

      Heterozygosity for Dcr-2L811fsX in a Scer\GAL4Act5C.PI Zzzz\repScer\UAS.T:Avic\GFP background results in increased Sindbis viral replication.

      (Avadhanula et al., 2009)
      Complementation and Rescue Data
      Rescued by

      Dcr-2L811fsX/Df(2R)BSC45 is rescued by Dcr-2+tKa

      (Wen et al., 2015)

      Dcr-2L811fsX/Df(2R)BSC45 is rescued by Dcr-2+tKa

      (Kemp et al., 2013)
      Comments

      The severely reduced fertility of Dcr-2L811fsX/Df(2R)BSC45 mutant males along with their sperm deficiency is rescued by combination with Dcr-2+tKa.

      (Wen et al., 2015)

      Dcr-2+tKa rescues the increased susceptibility of Dcr-2L811fsX/Df(2R)BSC45 mutant flies to IIV-6 infection.

      (Kemp et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (19)
      Reported As
      Symbol Synonym
      Dcr-2L811FSX
      (Deddouche et al., 2008)
      Dcr-2L811FXS
      (Avadhanula et al., 2009)
      Dcr-2L811Fsx
      (Mueller et al., 2010, Czech et al., 2008)
      Dcr-2L811X
      (Hatfield et al., 2005)
      Dcr-2L811fsX
      (Segrist et al., 2021, Iki et al., 2020, Ahlers et al., 2019, Genenncher et al., 2018, Liu et al., 2018, Teixeira et al., 2017, Guida et al., 2016, Lim et al., 2014, Durdevic et al., 2013, Kemp et al., 2013, Lawlor et al., 2012, Toledano et al., 2012, Poulton et al., 2011, Okamura et al., 2008, Galiana-Arnoux et al., 2006, Grimaud et al., 2006, Meyer et al., 2006, Vagin et al., 2006, van Rij et al., 2006, Wang et al., 2006, Wang et al., 2006)
      Dcr-2L811fsx
      (Eyk et al., 2019, Habayeb et al., 2009)
      Dcr2KO
      (Chow et al., 2017)
      Dcr2L811fsX
      (Coll et al., 2018, Chow et al., 2017, Lamiable et al., 2016)
      Dicer-2L811fsX
      (Berdnik et al., 2008)
      dcr-2L811Ex
      (Ashraf et al., 2006)
      dcr-2L811X
      (Shcherbata et al., 2007)
      dcr-2L811fs
      (Martin et al., 2009)
      dcr-2L811fsX
      (Donelick et al., 2020, Lee et al., 2020, Chak et al., 2015, Wen et al., 2015, Zhang et al., 2015, Lim et al., 2013, Perrat et al., 2013, Fukunaga et al., 2012, Han et al., 2011, Pek and Kai, 2011, Pek and Kai, 2011, Ghildiyal et al., 2008, Lim et al., 2008, Zhou et al., 2008, Förstemann et al., 2007, Horwich et al., 2007, Lim and Kai, 2007, Lim and Kai, 2007, Pelisson et al., 2007, Tomari et al., 2007, Bilen et al., 2006, Vagin et al., 2006, Matranga et al., 2005, Tomari et al., 2004)
      dcr2L811fsX
      (Dufourt et al., 2011, Lawlor et al., 2011, Okamura et al., 2011, Yu et al., 2011, Fagegaltier et al., 2009)
      dicer-2L811fsX
      (Harris et al., 2011)
      dicer-2L811fsx
      (Kandasamy et al., 2017, Kandasamy and Fukunaga, 2016)
      dicer-2null
      (Kandasamy et al., 2017, Kandasamy and Fukunaga, 2016)
      dicer2L811fsX
      (Martins et al., 2019)
      Name Synonyms
      Suppressor(GMR-wIR)A293
      (Lee et al., 2004)
      Secondary FlyBase IDs
        References (72)