Leu 811 is replaced by FGIRSLCWIVAARRTPTWX.
The Leucine at residue 811 is replaced by FGIRSLCWIVAARRTPTWX.
Dcr-2L811fsX adults systemically infected, but not orally infected, with Drosophila C Virus (DCV) show increased viral load compared to controls; oral infection with Sindbis virus (SINV) does not lead to increased viral load compared to controls.
Dcr-2L811fsX homozygotes do not show significant differences in viral load in the head upon infection with ZIKV, as compared to infected controls.
The fertility of Dcr-2L811fsX/Df(2R)BSC45 mutant males is severely reduced compared to wild-type controls (female fertility is not affected) due decreased number of mature sperm in their seminal vesicle. The spermatid nuclear alignment as well as the alignment of the investment cones in thee testes is disrupted.
The survival rate of DIP1EY02625 mutants after DCV infection is 17% compared to controls. Median survival is 5-6 days.
Compared with DCV-infected wild-type flies, Dcr-2L811fsX homozygotes have a much higher viral load.
Dcr-2L811fsX/Df(2R)BSC45 flies injected with Invertebrate Iridescent Virus type 6 (IIV-6) die earlier than IIV-6 infected wild-type controls. A fraction of Dcr-2L811fsX/Df(2R)BSC45 flies injected with buffer also die in the course of the experiment, indicating an increased sensitivity to stress associated with the mutation.
Dcr-2L811fsX mutant flies are more susceptible to non-virulent Flock house virus (FHV) mutant infection than wild-type. The FHV mutant that lacks viral protein FHV\B2, a known RNAi suppressor, is not pathogenic in wild-type flies, but Dcr-2L811fsX mutants show decreased survival after infection. Unlike the case in wild-type flies, the FHV FHV\B2 mutant replicates to high levels in Dcr-2L811fsX mutants.
More than 60% of cells in anaphase mutant wing discs contain lagging chromosomes.
Dcr-2L811fsX/+ significantly suppresses the position effect variegation of the Sb1 allele that is seen in the T(2;3)SbV chromosome.
Dcr-2L811fsX/Dcr-2R416X suppresses the position effect variegation of the w gene that is seen in the In(1)wm4 chromosome.
Homozygous projection neurons show normal dendrite and axon targeting.
Dcr-2L811fsX mutant germline stem cells (GSCs) do not exhibit any maintenance defects when clones are induced either during larval/pupal stages or during adult stages.
Mutant flies are more susceptible to infection with RNA viruses (Flock House virus, Drosophila C virus or Sindbis virus) than control flies.
Embryos derived from Dcr-2L811fsX mothers exhibit normal cellularization and net lipid-droplet transport.
Dcr-2L811fsX homozygous flies fail to produce siRNA from a transfected construct.
Dcr-2L811fsX flies show increased mortality and an increased level of viral accumulation following infection with both Flock house virus (FHV) and cricket paralysis virus (CrPV).
Mutant flies are hypersensitive to infection with Cricket Paralysis virus (CrPV); the median survival after inoculation with 350 TCID50 of CrPV is reduced compared to controls.
When Dcr-2L811fsX germ-line clones are made a normal progression of egg chambers is seen.
Dcr-2[+]/Dcr-2L811fsX is a non-enhancer of visible phenotype of Scer\GAL4da.G32, Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG
Dcr-2[+]/Dcr-2L811fsX is a non-enhancer of visible phenotype of Scer\GAL4da.G32, Zzzz\CTGrCUG.99.UAS.Tag:MYC,Tag:FLAG
Dcr-2L811fsX, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF is a non-enhancer of increased cell death phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF
Dcr-2[+]/Dcr-2L811fsX is a suppressor | partially of partially lethal - majority live phenotype of ctA, lncRNA:flamA
Dcr-2L811fsX is a suppressor of visible phenotype of wdsRNA.GMR/wdsRNA.GMR
Dcr-2[+]/Dcr-2L811fsX is a suppressor | partially of visible phenotype of Scer\GAL4GMR.PF, Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG, Zzzz\CTGrCUG.99.UAS.Tag:MYC,Tag:FLAG
Dcr-2L811fsX is a suppressor of abnormal eye color phenotype of wIR.GMR
Dcr-2L811fsX is a suppressor | somatic clone of abnormal eye color phenotype of wIR.GMR
Dcr-2L811fsX/Dcr-2L811fsX is a suppressor of abnormal eye color phenotype of wdsRNA.GMR/wdsRNA.GMR
Dcr-2[+]/Dcr-2L811fsX is a suppressor | partially of increased cell death phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, Diap1dsRNA.UAS
Dcr-2[+]/Dcr-2L811fsX is a non-suppressor of visible phenotype of Scer\GAL4da.G32, Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG
Dcr-2[+]/Dcr-2L811fsX is a non-suppressor of visible phenotype of Scer\GAL4da.G32, Zzzz\CTGrCUG.99.UAS.Tag:MYC,Tag:FLAG
Dcr-2L811fsX, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF is a non-suppressor of increased cell death phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF
Dcr-1Q1147X, Dcr-2L811fsX has decreased cell number | somatic clone | larval stage phenotype
Dcr-1Q1147X, Dcr-2L811fsX has decreased cell number | somatic clone | pupal stage phenotype
Dcr-2L811fsX, wIR.GMR has eye phenotype, suppressible | partially by Dcr-2E1617K.t7.2
Dcr-2L811fsX, wIR.GMR has pigment cell phenotype, suppressible | partially by Dcr-2E1617K.t7.2
Dcr-2L811fsX, wIR.GMR has eye phenotype, suppressible by Dcr-2E1210V.t7.2
Dcr-2L811fsX, wIR.GMR has pigment cell phenotype, suppressible by Dcr-2E1210V.t7.2
Dcr-2L811fsX, wIR.GMR has eye phenotype, suppressible | partially by Dcr-2E1371K.t7.2
Dcr-2L811fsX, wIR.GMR has pigment cell phenotype, suppressible | partially by Dcr-2E1371K.t7.2
Dcr-2L811fsX, wIR.GMR has eye phenotype, non-suppressible by Dcr-2E1371K.E1617K.t7.2
Dcr-2L811fsX, wIR.GMR has pigment cell phenotype, non-suppressible by Dcr-2E1371K.E1617K.t7.2
Dcr-2[+]/Dcr-2L811fsX is an enhancer of phenotype of Zzzz\repScer\UAS.T:Avic\GFP/Sindbis\repUAS.GFP
Dcr-2[+]/Dcr-2L811fsX is a non-enhancer of tergite phenotype of Scer\GAL4da.G32, Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG
Dcr-2[+]/Dcr-2L811fsX is a non-enhancer of tergite phenotype of Scer\GAL4da.G32, Zzzz\CTGrCUG.99.UAS.Tag:MYC,Tag:FLAG
Dcr-2L811fsX, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF is a non-enhancer of eye phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF
Dcr-2L811fsX, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF is a non-enhancer of retina phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF
Dcr-2L811fsX is a suppressor of eye phenotype of wdsRNA.GMR/wdsRNA.GMR
Dcr-2[+]/Dcr-2L811fsX is a suppressor | partially of eye phenotype of Scer\GAL4GMR.PF, Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG, Zzzz\CTGrCUG.99.UAS.Tag:MYC,Tag:FLAG
Dcr-2L811fsX/Dcr-2L811fsX is a suppressor of eye phenotype of wdsRNA.GMR/wdsRNA.GMR
Dcr-2L811fsX/Dcr-2L811fsX is a suppressor of wing phenotype of sdFab-X
Dcr-2[+]/Dcr-2L811fsX is a suppressor | partially of eye phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, Diap1dsRNA.UAS
Dcr-2L811fsX is a suppressor of eye phenotype of wIR.GMR
Dcr-2L811fsX is a suppressor of pigment cell phenotype of wIR.GMR
Dcr-2[+]/Dcr-2L811fsX is a non-suppressor of tergite phenotype of Scer\GAL4da.G32, Zzzz\CAG99.UAS.Tag:MYC,Tag:FLAG
Dcr-2[+]/Dcr-2L811fsX is a non-suppressor of tergite phenotype of Scer\GAL4da.G32, Zzzz\CTGrCUG.99.UAS.Tag:MYC,Tag:FLAG
Dcr-2L811fsX, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF is a non-suppressor of eye phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF
Dcr-2L811fsX, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF is a non-suppressor of retina phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF
Dcr-1Q1147X, Dcr-2L811fsX has female germline stem cell | somatic clone | larval stage phenotype
Dcr-1Q1147X, Dcr-2L811fsX has female germline stem cell | somatic clone | pupal stage phenotype
Dcr-2L811fsX heterozygosity partially suppresses the mortality of flamA, ctA double homozygotes.
The eyes of wdsRNA.GMR transgenic female flies homozygous for Dcr-2L811fsX are similar to that of wild-type.
A Dcr-2L811fsX homozygous mutant background suppresses the developmental lethality associated with expression of Disc\RFP(CTG)130.Scer\UAS and Disc\RFPCAG100.DsRed2.Scer\UAS in the developing muscle (under the control of Scer\GAL4how-24B).
A Dcr-2L811fsX homozygous mutant background suppresses the organismal lethality associated with 30 minute heat-shock induced expression of Disc\RFPCAG250.DsRed2.Scer\UAS and Disc\RFP(CTG)250.Scer\UAS (under the control of Scer\GAL4hs.PB).
wIR.GMR eyes that are also homozygous for Dcr-2L811fsX show strong suppression of the pale eye colour phenotype.
Flies carrying wIR.GMR in which the eyes are homozygous for Dcr-2L811fsX have dark red eyes.
Flies carrying one copy of wdsRNA.GMR in a homozygous Dcr-2L811fsX background have red eyes.
Dcr-1Q1147X Dcr-2L811fsX double mutants show strong germline stem cell maintenance (GSC) defects when GSC clones are induced during late larval/early pupal stages.
Eye degeneration due to expression of Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 in the eye (under the control of Scer\GAL4GMR.PF) is unaffected by a Dcr-2L811fsX background.
The wing phenotype of sdFab-X flies is suppressed in sdFab-X;Dcr-2L811fsX flies.
A Dcr-2L811fsX/+ background partially suppresses the small eye phenotype that is seen when thdsRNA.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF.
Dcr-2L811fsX; Dcr-1Q1147X double mutants show no segment polarity defects.
Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG expression using Scer\GAL4da.G32 in the presence of heterozygous Dcr-2L811fsX does not give significantly different phenotype severity compared to Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG expression in a wild-type Dcr-2 background.
Zzzz\CAGrCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG expression using Scer\GAL4da.G32 in the presence of heterozygous Dcr-2L811fsX does not give significantly different phenotype severity compared to Zzzz\CAGrCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG expression in a wild-type Dcr-2 background.
Heterozygous Dcr-2L811fsX suppresses the eye-phenotype resulting from the co-expression of Zzzz\CAG99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG and Zzzz\CAGrCUG.99.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4GMR.PF. In addition, the overall disruption observed in patterning of the internal structure of the eye is significantly reduced in the presence of heterozygous Dcr-2L811fsX as revealed by sections through adult eyes.
Heterozygosity for Dcr-2L811fsX in a Scer\GAL4Act5C.PI Zzzz\repScer\UAS.T:Avic\GFP background results in increased Sindbis viral replication.
Dcr-2L811fsX/Df(2R)BSC45 is rescued by Dcr-2+tKa
Dcr-2L811fsX/Df(2R)BSC45 is rescued by Dcr-2+tKa
The severely reduced fertility of Dcr-2L811fsX/Df(2R)BSC45 mutant males along with their sperm deficiency is rescued by combination with Dcr-2+tKa.
Dcr-2+tKa rescues the increased susceptibility of Dcr-2L811fsX/Df(2R)BSC45 mutant flies to IIV-6 infection.