In contrast to their different responses to wild-type females, CheB42aΔ5-68 and wild-type males attempt to copulate with heat-shocked females expressing traScer\UAS.cFa under the control of Scer\GAL4hs.PB with indistinguishable frequency and kinetics, suggesting that the behavioural effect of CheB42a depends on the presence of the major female cuticular hydrocarbons.
Partially-transformed males, where traScer\UAS.cFa under the control of Scer\GAL4oeE is expressed in the oenocytes, elicit significant courtship responses both from controls and CheB42aΔ5-68 males. Mutant males outperform controls specifically in the kinetics and frequency of attempted copulations.
Co-expression of shi1.Scer\UAS in feminised males expressing traScer\UAS.cFa under the control of Scer\GAL455B by shifting copulating pairs of flies to the restrictive temperature either late or early during copulation has little influence of the fertility of the males but highly decreases their ability to repress female remating.
Males expressing traScer\UAS.cFa under the control of Scer\GAL4103Y in an oc1 background do not show feminisation of walking behaviour; the number of start/stop bouts is not greater than in control males.
The abnormal copulation duration and reduced fertility of feminised XY males expressing traScer\UAS.cFa under the control of Scer\GAL455B is rescued by expression of Scer\GAL80Cha.PK. In addition, for these rescued males, no case of remating within 24 hours is detected in wild-type females mated to them.