Neuronal hyperactivation of mdIV neurons through expression of Bhal\NaChBacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4ppk.1.9 display severely premature intermediate surfacing transition (IST) behavior (emerging from the food transiently to to roam the food surface, prior to complete food exit) compared to wild type. The mutant larvae also display a premature shift in thermotactic preference behavior (wild type larvae change the way they respond to temperature changes when they go from feeding to wandering) compared to wild type. However, eventual food exit does not occur significantly later than in controls. The resulting adult flies are smaller in size than their wild type counterparts.
Flies expressing Bhal\NaChBacScer\UAS.T:Avic\GFP-EGFP under the control of either Scer\GAL4c673a or Scer\GAL4fru-GAL4 have a decrease in fat levels compared to control flies. The abdominal fat cells have either no fat droplets or only a few small ones. The food intake of these flies is not significantly different from that of controls. These flies show an increase in CO[] emission compared to control flies.
Flies expressing Bhal\NaChBacScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4fru-GAL4 show massive autophagic vesicles in fat and malpighian tubule cells (these are not seen in control flies).