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General Information
Symbol
Dmel\Tdc2RO54
Species
D. melanogaster
Name
FlyBase ID
FBal0189582
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

G6688290A

Amino acid change:

E51K | Tdc2-PA

Reported amino acid change:

E51K

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: E51K. Point mutation in highly conserved pyridoxal-dependent decarboxylase conserved domain.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Tdc2RO54/Df(2R)42 mutant adult females display defects in trimming of posterior follicle cells and produce fewer trimmed eggs.

Virgin Tdc2RO54 mutant females show increased receptivity compared to wild type virgin females. Mated Tdc2RO54 females lay very few eggs and remain highly receptive after copulation, displaying low levels of rejection behaviors such as ovipositor extrusion compared with mated controls. Wild type males court Tdc2RO54 mated females significantly more than wild type mated females.

Mutants show decreased activity and decreased circadian rhythmicity.

TbhnM18/+ larvae have a significant reduction in the distance they crawl, relative to controls; locomotion tends to be further reduced in homozygotes.

Tdc2RO54 mutant third instar larvae exhibit an increase in the number of synaptopods (dynamic filopodia-like extensions).

Tdc2RO54 mutant neuromuscular junctions exhibit a wild-type response to the addition of 10υM octopamine with an increase in the number of synaptopods.

Tdc2RO54 mutant third instar larval neuromuscular junctions exhibit a decrease in the number of type II boutons and terminal branches.

Tdc2RO54 mutant third instar larvae exhibit a 15% increase in locomotor speed in response to starvation for 2 hours, compared to a 36% increase in wild-type.

Tdc2RO54 male adult flies show reduced locomotor activity compared to control flies; this effect is much greater in homozygotes but can still be observed in heterozygotes.

Tdc2RO54 homozygous adult flies are hypersensitive to cocaine; these flies show at least a 5X increase in responsiveness relative to controls although the types of behavior induced are indistinguishable from controls. Tdc2RO54 heterozygotes also show significant cocaine sensitivity but this is less severe than homozygotes. The Tdc2RO54 flies show similar behavioral sensitization to repeated doses of cocaine to control flies.

Tdc2RO54 females fail to lay eggs. As mature eggs and egg remnants can be found in the calyx, lateral and common oviduct, it appears that these mutants achieve ovulation but not oviposition. This phenotype can be partially rescued by feeding with tyramine and octopamine. Tdc2RO54/Df(2R)42 transheterozygotes also fail to lay eggs. Tdc2RO54 flies have reduced levels of tyramine and octopamine in the brain compared to wild type.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT suppressed by
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Expression of Tdc1Scer\UAS.cCa under the control of Scer\GAL4Tdc2.PC results in complete suppression of the cocaine sensitivity phenotype of Tdc2RO54 flies.

Expression of Tdc1Scer\UAS.cCa, under the control of Scer\GAL4Tdc2.PC, completely rescues the egg retention phenotype of Tdc2RO54 mutants. However, expression of this transgene fails to rescue the reduced neural tyramine and octopamine phenotype.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

Expression of Tdc2Scer\UAS.cCb, under the control of Scer\GAL4Tdc2.PC, partially rescues the egg retention phenotype of Tdc2RO54 mutants. This expression also increases levels of tyramine and octopamine in the brains of Tdc2RO54 flies.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

T. Schupbach

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (13)