FlyBase Allele Report: Dmel\Gαo[GD8640]

General Information

Symbol

Dmel\Gαo^GD8640

Species

D. melanogaster

Name

FlyBase ID

FBal0207066

Feature type

allele

Associated gene

Dmel\Gαo

Associated Insertion(s)

Carried in Construct

P{GD8640}

Also Known As

UAS-RNAi-Gαo, UAS-Go-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD8640}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD8640

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Gαo^GD8640 under the control of Scer\GAL4^GH146 or Scer\GAL4^NP2631, but not Scer\GAL4^NP0225, in combination with a Dicer-2 transgene to enhance RNAi efficiency, significantly reduces short term aversive olfactory memory in adults, compared to controls.

(Zhou et al., 2019)

Expression of Gαo^GD8640 under the control of Scer\GAL4^Bx-MS1096 results in a folded wing phenotype (78% penetrance), due to a failure of wing expansion after eclosion. The flies also show a multiple wing hair phenotype.

(Lin and Katanaev, 2013)

The number of natural synaptopods (motile filopodia-like extensions) at type II neuromuscular junctions is significantly increased compared to wild type in third instar larvae expressing G-oα47A^GD8640 under the control of Scer\GAL4^Tdc2.PC. The number of type II boutons at the neuromuscular junction is also increased in these larvae.

The number of type I boutons at the neuromuscular junction is normal in third instar larvae expressing G-oα47A^GD8640 under the control of Scer\GAL4^Tdc2.PC.

The number of type I boutons at the neuromuscular junction is increased compared to wild type in third instar larvae expressing G-oα47A^GD8640 under the control of either Scer\GAL4^futsch-C380 or Scer\GAL4^BG439.

(Koon and Budnik, 2012)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8640

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Gαo^GD8640, Scer\GAL4^Bx-MS1096 has abnormal planar polarity phenotype, suppressible by kermit^KK101898, Scer\GAL4^Bx-MS1096

(Lin and Katanaev, 2013)

Gαo^GD8640, Scer\GAL4^Bx-MS1096 has visible phenotype, suppressible | partially by kermit^SH0225/kermit[+]

(Lin and Katanaev, 2013)

Gαo^GD8640, Scer\GAL4^Bx-MS1096 has abnormal planar polarity phenotype, suppressible | partially by kermit^SH0225/kermit[+]

(Lin and Katanaev, 2013)

Gαo^GD8640, Scer\GAL4^Bx-MS1096 has abnormal planar polarity phenotype, suppressible | partially by kermit^ex31/kermit[+]

(Lin and Katanaev, 2013)

NOT Enhancer of

Statement

Reference

Gαo^GD8640, Scer\GAL4^Bx-MS1096 is a non-enhancer of abnormal planar polarity phenotype of Scer\GAL4^Bx-MS1096, kermit^UAS.cLa

(Lin and Katanaev, 2013)

NOT Suppressor of

Statement

Reference

Gαo^GD8640, Scer\GAL4^Bx-MS1096 is a non-suppressor of abnormal planar polarity phenotype of Scer\GAL4^Bx-MS1096, kermit^UAS.cLa

(Lin and Katanaev, 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Gαo^GD8640, Scer\GAL4^Bx-MS1096 has wing phenotype, suppressible | partially by kermit^SH0225/kermit[+]

(Lin and Katanaev, 2013)

Gαo^GD8640, Scer\GAL4^Bx-MS1096 has wing hair | increased number phenotype, suppressible | partially by kermit^SH0225/kermit[+]

(Lin and Katanaev, 2013)

Gαo^GD8640, Scer\GAL4^Bx-MS1096 has wing hair | increased number phenotype, suppressible | partially by kermit^ex31/kermit[+]

(Lin and Katanaev, 2013)

Gαo^GD8640, Scer\GAL4^Bx-MS1096 has wing hair | increased number phenotype, suppressible by kermit^KK101898, Scer\GAL4^Bx-MS1096

(Lin and Katanaev, 2013)

NOT Enhancer of

Statement

Reference

Gαo^GD8640, Scer\GAL4^Bx-MS1096 is a non-enhancer of wing hair phenotype of Scer\GAL4^Bx-MS1096, kermit^UAS.cLa

(Lin and Katanaev, 2013)

Gαo^GD8640, Scer\GAL4^Bx-MS1096 is a non-enhancer of wing hair | increased number phenotype of Scer\GAL4^Bx-MS1096, kermit^UAS.cLa

(Lin and Katanaev, 2013)

NOT Suppressor of

Statement

Reference

Gαo^GD8640, Scer\GAL4^Bx-MS1096 is a non-suppressor of wing hair phenotype of Scer\GAL4^Bx-MS1096, kermit^UAS.cLa

(Lin and Katanaev, 2013)

Gαo^GD8640, Scer\GAL4^Bx-MS1096 is a non-suppressor of wing hair | increased number phenotype of Scer\GAL4^Bx-MS1096, kermit^UAS.cLa

(Lin and Katanaev, 2013)

Additional Comments

Genetic Interactions

Statement

Reference

kermit^SH0225/+ partially suppresses (to 22% penetrance) the folded wing phenotype caused by expression of Gαo^GD8640 under the control of Scer\GAL4^Bx-MS1096.

The multiple wing hair phenotype seen in flies expressing Gαo^GD8640 under the control of Scer\GAL4^Bx-MS1096 is partially suppressed if they are heterozygous for either kermit^SH0225 or kermit^ex31, and is almost completely suppressed by co-expression of kermit^KK101898.

Co-expression of Gαo^GD8640 has no effect on the wing defects caused by expressing kermit^Scer\UAS.cLa under the control of Scer\GAL4^Bx-MS1096.

(Lin and Katanaev, 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v19124

w¹¹¹⁸; P{GD8640}v19124

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8640

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

G-oα47A^GD8640

Gαo^GD8640

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

Open Close