Pan-neural expression of NetB^Scer\UAS.cHa under the control of Scer\GAL4^sca-109-68 dramatically reduces apoptosis in the thoracic and abdominal segments of the ventral nerve cord.

When Df(1)NetAB^Δ mutant females expressing NetB^Scer\UAS.cHa under the control of Scer\GAL4^sli.PS are crossed to wild type males there is a reduction in the proportion of eggs that hatch compared to wild type.

Expression of NetB^Scer\UAS.cHa using Scer\GAL4^sli.PS results in the dendrites of the motoneuron labelled by Scer\GAL4^{VGlut-VGN9281} having a wild type distribution.

The projections from the pCC neurons on either side of the midline are attracted toward the midline in 11 hour embryos expressing NetB^Scer\UAS.cHa under the control of Scer\GAL4^unspecified. 14 hour embryos expressing NetB^Scer\UAS.cHa under the control of Scer\GAL4^unspecified show collapse of the longitudinal tracts at the midline in 40% of hemisegments, and in other hemisegments the longitudinal tracts are shifted closer to the midline.

When expression is driven by Scer\GAL4^GMR.PF, retinal axons stall in the optic stalk, spread abnormally or criss-cross each other along the D/V axis. Photoreceptor axons also collapse onto each other along the A/P axis. They fail to innervate their target region properly. When expression is driven in clones in the anterior, undifferentiated portion of the eye disc or in the lamina anlage by Scer\GAL4^Act5C.PP there is no effect on retinal fibers. However when clones fall in the differentiating portion of the lamina, mild hypoinnervation and irregular spacing of retinal fibers occurs.

Scer\GAL4^F63-mediated expression elicits inappropriate innervation by the transverse nerve (ectopic innervation of muscles 7 and 6 and occasionally 13). Scer\GAL4^how-24B-mediated expression sometimes causes the entire SN to stall as a clump of growth cones either within the CNS or in the ventral muscle region The SNc often extends for only a short distance and then ends in a clump or fails altogether. When the SN reaches the lateral muscle domain the SNa often fails to branch. Loss of Sema-2a expression allows both the SNa and SNc to project normally. Scer\GAL4^A51-mediated expression in muscle 5 specifically repels the lateral branch of the SNa, the nerve sometimes takes a circuitous path to muscle 8 without innervating muscle 5. Scer\GAL4^how-24B-mediated expression allows the axons of the transverse nerve to ectopically innervate muscles 7 and 6.

Df(1)KA9 and Df(1)RK5 embryos exhibit variable defects in the formation of commissural pathways, Scer\GAL4^rho.PL induced expression of P{UAS-NETB} significantly rescued the CNS phenotype. Scer\GAL4^sca-537.4 induced expression leads to a variety of CNS defects; reductions in the number of axons in commissural bundles and more extremely missing commissures.

NetB^UAS.cHa, Scer\GAL4^how-24B has larval transverse nerve phenotype, suppressible by fra³

NetB^UAS.cHa, Scer\GAL4^how-24B has larval transverse nerve phenotype, suppressible by fra⁴

NetB^UAS.cHa, Scer\GAL4⁶⁰⁵, fra³/fra⁴ has dMP2 neuron phenotype

NetB^UAS.cHa, Scer\GAL4^how-24B, Sema2a⁰³⁴⁷⁴ has larval segmental nerve phenotype

NetB^UAS.cHa, Scer\GAL4^how-24B, Sema2a⁰³⁴⁷⁴ has larval abdominal segmental nerve phenotype