FlyBase Allele Report: Scer\GAL4[sim.P3.7]

General Information

Symbol

Scer\GAL4^sim.P3.7

Species

S. cerevisiae

Name

FlyBase ID

FBal0060666

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{GAL4-sim.3.7}

Also Known As

sim-Gal4, simGAL4, P[sim-Gal4], 3.7sim-Gal4, single-minded (sim)-Gal4, simG4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Xiao et al., 1996)

Progenitor genotype

Carried in construct

P{GAL4-sim.3.7}

Cytology

Description

A 3.7kb BamHI fragment of sim 5' flanking DNA containing the early sim promoter and 5' flanking sequences (encodes 18 amino acids of coding sequence) regulates expression of a GAL4 driver.

(Xiao et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

sim

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic midline of ventral nerve cord

(Bloomington Drosophila Stock Center, 2017)

embryonic stage 10 -- 17

larval ventral nerve cord | precursor

(Xiao et al., 1996)

ventral midline of embryo

(Xiao et al., 1996)

embryonic stage 11

ventral midline of embryo

Comment: all cells

(Wheeler et al., 2006)

embryonic stage 11 -- 12

ventral midline of embryo

(Fontana and Crews, 2012)

embryonic stage 13

ventral midline of embryo

Comment: all cells

(Wheeler et al., 2006)

embryonic stage 16

ventral midline of embryo

(Fontana and Crews, 2012)

embryonic stage 17

ventral midline of embryo

Comment: all cells

(Wheeler et al., 2006)

Additional Information

Statement

Reference

Drives expression in cells of the ventral midline of the embryonic CNS.

(FlyBase Curators, 2017)

Midline expression is present in all midline cells from segments S1 to A8/9.

(Fontana and Crews, 2012)

Expression assayed at stages 9, 11, 13, and 17. Expression may be continuous between assayed stages in some tissues.

(Wheeler et al., 2006)

In embryos, expression specific to CNS midline precursor cells (CMP); not expressed in ventral ectodermal cells.

(Xiao et al., 1996)

Marker for

ventral midline of embryo

(Xiao et al., 1996)

Reflects expression of

sim

(Bloomington Drosophila Stock Center, 2017, Wheeler et al., 2006, Xiao et al., 1996)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Abcd1^HMS02382

(Gordon et al., 2018)

abnormal neuroanatomy, with Elovl7^GD16713

(Gordon et al., 2018)

abnormal neuroanatomy, with Elovl7^HMC03112

(Gordon et al., 2018)

abnormal neuroanatomy, with en^UAS.cGa

(Watson et al., 2011)

abnormal neuroanatomy, with hh^UAS.cAa

(Watson et al., 2011)

abnormal neuroanatomy, with jing^UAS.cSa

(Sedaghat and Sonnenfeld, 2002)

abnormal neuroanatomy, with sim^UAS.cXa

(Sedaghat and Sonnenfeld, 2002)

abnormal neuroanatomy, with tgo^Δc.UAS

(Sonnenfeld et al., 2005)

abnormal neuroanatomy | embryonic stage 13, with N^{Intra1768.UAS.Tag:FLAG}

(Zhang et al., 2011)

abnormal neuroanatomy | embryonic stage 13, with spi^s.UAS

(Zhang et al., 2011)

abnormal neuroanatomy | embryonic stage 13, with Su(H)^{VP16.UAS.Tag:MYC}

(Zhang et al., 2011)

abnormal neuroanatomy | embryonic stage 16, with N^{Intra1768.UAS.Tag:FLAG}

(Zhang et al., 2011)

abnormal neuroanatomy | embryonic stage 16, with spi^s.UAS

(Zhang et al., 2011)

abnormal neuroanatomy | embryonic stage 16, with Su(H)^{VP16.UAS.Tag:MYC}

(Zhang et al., 2011)

abnormal neuroanatomy | embryonic stage, with D^EnR.UAS

(Shen et al., 2013)

abnormal neuroanatomy | embryonic stage, with D^EnR.UAS, D^r72/Df(3L)fz-GS1a

(Shen et al., 2013)

abnormal neuroanatomy | embryonic stage, with D^UAS.WT

(Shen et al., 2013)

abnormal neuroanatomy | embryonic stage, with D^ΔHMG.UAS

(Shen et al., 2013)

abnormal neuroanatomy | embryonic stage, with D^ΔHMG.UAS, D^r72/Df(3L)fz-GS1a

(Shen et al., 2013)

abnormal neuroanatomy | embryonic stage, with Mmus\Sox2^{EnR.UAS.Tag:MYC}

(Shen et al., 2013)

abnormal neuroanatomy | embryonic stage, with Mmus\Sox2^UAS.EYFP

(Shen et al., 2013)

abnormal neuroanatomy | embryonic stage, with Mmus\Sox2^{ΔHMG.UAS.EYFP,Tag:MYC}

(Shen et al., 2013)

decreased cell number | adult stage, with Abcd1^HMS02382

(Gordon et al., 2018)

decreased cell number | adult stage, with Elovl7^HMC03112

(Gordon et al., 2018)

viable, with Abcd1^HMS02382

(Gordon et al., 2018)

Phenotype Manifest In

axon, with sli^UAS.cBa

(Battye et al., 1999)

axon | embryonic stage, with D^EnR.UAS

(Shen et al., 2013)

axon | embryonic stage, with D^ΔHMG.UAS

(Shen et al., 2013)

central nervous system, with sli^UAS.cBa

(Battye et al., 1999)

embryonic brain, with jing^UAS.cSa

(Sedaghat and Sonnenfeld, 2002)

embryonic brain, with sim^UAS.cXa

(Sedaghat and Sonnenfeld, 2002)

larval anterior commissure | embryonic stage, with Inx7^RNAi.UAS

(Ostrowski et al., 2008)

larval central nervous system, with D^EnR.UAS

(Shen et al., 2013)

larval central nervous system, with D^EnR.UAS, D^r72/Df(3L)fz-GS1a

(Shen et al., 2013)

larval central nervous system, with D^UAS.WT

(Shen et al., 2013)

larval central nervous system, with D^ΔHMG.UAS

(Shen et al., 2013)

larval central nervous system, with D^ΔHMG.UAS, D^r72/Df(3L)fz-GS1a

(Shen et al., 2013)

larval central nervous system, with Mmus\Sox2^{EnR.UAS.Tag:MYC}

(Shen et al., 2013)

larval central nervous system, with Mmus\Sox2^UAS.EYFP

(Shen et al., 2013)

larval central nervous system, with Mmus\Sox2^{ΔHMG.UAS.EYFP,Tag:MYC}

(Shen et al., 2013)

larval longitudinal connective, with jing^UAS.cSa

(Sedaghat et al., 2002)

larval longitudinal connective, with sli^UAS.cBa

(Battye et al., 1999)

larval longitudinal connective, with tgo^Δc.UAS

(Sonnenfeld et al., 2005)

larval longitudinal connective | embryonic stage, with D^EnR.UAS

(Shen et al., 2013)

larval longitudinal connective | embryonic stage, with D^ΔHMG.UAS

(Shen et al., 2013)

larval longitudinal connective | embryonic stage, with Inx7^RNAi.UAS

(Ostrowski et al., 2008)

larval posterior commissure | embryonic stage, with Inx7^RNAi.UAS

(Ostrowski et al., 2008)

larval somatic muscle cell | ectopic, with sli², sli^UAS.cKa

(Kramer et al., 2001)

larval ventral nerve cord | embryonic stage, with Inx7^RNAi.UAS

(Ostrowski et al., 2008)

midline glial cell, with en^UAS.cGa

(Watson et al., 2011)

midline glial cell, with hh^UAS.cAa

(Watson et al., 2011)

midline glial cell | embryonic stage 13 | increased number, with N^{Intra1768.UAS.Tag:FLAG}

(Zhang et al., 2011)

midline glial cell | embryonic stage 13 | increased number, with spi^s.UAS

(Zhang et al., 2011)

midline glial cell | embryonic stage 13 | increased number, with Su(H)^{VP16.UAS.Tag:MYC}

(Zhang et al., 2011)

midline glial cell | embryonic stage 14, with Mmmm\ras^N17.UAS

(Settle et al., 2003)

midline glial cell | embryonic stage 16 | increased number, with Egfr^U.A887T.UAS

(Settle et al., 2003)

midline glial cell | embryonic stage 16 | increased number, with N^{Intra1768.UAS.Tag:FLAG}

(Zhang et al., 2011)

midline glial cell | embryonic stage 16 | increased number, with Rat\Neu^NT.UAS

(Settle et al., 2003)

midline glial cell | embryonic stage 16 | increased number, with Rat\Neu^YB.UAS

(Settle et al., 2003)

midline glial cell | embryonic stage 16 | increased number, with Rat\Neu^YC.UAS

(Settle et al., 2003)

midline glial cell | embryonic stage 16 | increased number, with Rat\Neu^YD.UAS

(Settle et al., 2003)

midline glial cell | embryonic stage 16 | increased number, with Rat\Neu^YE.UAS

(Settle et al., 2003)

midline glial cell | embryonic stage 16 | increased number, with spi^s.UAS

(Zhang et al., 2011)

midline glial cell | embryonic stage 16 | increased number, with Su(H)^{VP16.UAS.Tag:MYC}

(Zhang et al., 2011)

muscle cell of A1-7 dorsal acute muscle 1, with sli², sli^UAS.cKa

(Kramer et al., 2001)

muscle cell of A1-7 dorsal acute muscle 2, with sli², sli^UAS.cKa

(Kramer et al., 2001)

muscle cell of A1-7 dorsal oblique muscle 1, with sli², sli^UAS.cKa

(Kramer et al., 2001)

muscle cell of A1-7 dorsal oblique muscle 2, with sli², sli^UAS.cKa

(Kramer et al., 2001)

muscle cell of A1-7 lateral oblique muscle 1, with sli², sli^UAS.cKa

(Kramer et al., 2001)

muscle cell of A1-7 ventral longitudinal muscle 3, with sli², sli^UAS.cKa

(Kramer et al., 2001)

muscle cell of A1-7 ventral longitudinal muscle 4, with sli², sli^UAS.cKa

(Kramer et al., 2001)

pigment cell, with Abcd1^HMS02382

(Gordon et al., 2018)

pigment cell, with Elovl7^HMC03112

(Gordon et al., 2018)

retina, with Abcd1^HMS02382

(Gordon et al., 2018)

retina, with Elovl7^GD16713

(Gordon et al., 2018)

retina, with Elovl7^HMC03112

(Gordon et al., 2018)

SP1 neuron, with sli^UAS.cBa

(Battye et al., 1999)

symmetrical commissure, with jing^UAS.cSa

(Sedaghat et al., 2002)

symmetrical commissure, with sli^UAS.cBa

(Battye et al., 1999)

symmetrical commissure, with tgo^Δc.UAS

(Sonnenfeld et al., 2005)

symmetrical commissure | embryonic stage, with D^EnR.UAS

(Shen et al., 2013)

symmetrical commissure | embryonic stage, with D^ΔHMG.UAS

(Shen et al., 2013)

tract | embryonic stage, with D^EnR.UAS

(Shen et al., 2013)

tract | embryonic stage, with D^ΔHMG.UAS

(Shen et al., 2013)

ventral nerve cord, with sli^UAS.cBa

(Battye et al., 1999)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^sim.P3.7, mam^ΔC has abnormal neuroanatomy | embryonic stage 16 phenotype, enhanceable by N^{Intra1768.UAS.Tag:FLAG}, Scer\GAL4^sim.P3.7

(Zhang et al., 2011)

NOT Enhanced by

Statement

Reference

Scer\GAL4^sim.P3.7, spi^s.UAS has abnormal neuroanatomy | embryonic stage 13 phenotype, non-enhanceable by mam^ΔC

(Zhang et al., 2011)

Scer\GAL4^sim.P3.7, spi^s.UAS has abnormal neuroanatomy | embryonic stage 16 phenotype, non-enhanceable by mam^ΔC

(Zhang et al., 2011)

NOT suppressed by

Statement

Reference

N^{Intra1768.UAS.Tag:FLAG}, Scer\GAL4^sim.P3.7 has abnormal neuroanatomy | embryonic stage 16 phenotype, non-suppressible by mam^ΔC

(Zhang et al., 2011)

Scer\GAL4^sim.P3.7, spi^s.UAS has abnormal neuroanatomy | embryonic stage 13 phenotype, non-suppressible by mam^ΔC

(Zhang et al., 2011)

Scer\GAL4^sim.P3.7, spi^s.UAS has abnormal neuroanatomy | embryonic stage 16 phenotype, non-suppressible by mam^ΔC

(Zhang et al., 2011)

Enhancer of

Statement

Reference

Scer\GAL4^sim.P3.7/Mmus\Sox2^{ΔHMG.UAS.EYFP,Tag:MYC} is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of D^r72/Df(3L)fz-GS1a

(Shen et al., 2013)

Mmus\Sox2^{EnR.UAS.Tag:MYC}/Scer\GAL4^sim.P3.7 is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of D^r72/Df(3L)fz-GS1a

(Shen et al., 2013)

N^{Intra1768.UAS.Tag:FLAG}, Scer\GAL4^sim.P3.7 is an enhancer of abnormal neuroanatomy | embryonic stage 16 phenotype of Scer\GAL4^sim.P3.7, mam^ΔC

(Zhang et al., 2011)

NOT Enhancer of

Statement

Reference

spi^s.UAS/Scer\GAL4^sim.P3.7 is a non-enhancer of abnormal neuroanatomy | embryonic stage 16 phenotype of mam^ΔC

(Zhang et al., 2011)

Suppressor of

Statement

Reference

Mmus\Sox2^UAS.EYFP/Scer\GAL4^sim.P3.7 is a suppressor of abnormal neuroanatomy | embryonic stage phenotype of D^r72/Df(3L)fz-GS1a

(Shen et al., 2013)

dlp^UAS.cBa/Scer\GAL4^sim.P3.7 is a suppressor | partially of abnormal neuroanatomy | embryonic stage phenotype of Sdc²³

(Chanana et al., 2009)

Scer\GAL4^sim.P3.7/Egfr^Elp.UAS is a suppressor | partially of abnormal neuroanatomy phenotype of jing³/Df(2R)ST1

(Sonnenfeld et al., 2004)

Scer\GAL4^sim.P3.7/Egfr^Elp.UAS is a suppressor of abnormal neuroanatomy phenotype of jing³

(Sonnenfeld et al., 2004)

Ras85D^V12.UAS/Scer\GAL4^sim.P3.7 is a suppressor of abnormal neuroanatomy phenotype of jing³

(Sonnenfeld et al., 2004)

NOT Suppressor of

Statement

Reference

spi^s.UAS/Scer\GAL4^sim.P3.7 is a non-suppressor of abnormal neuroanatomy | embryonic stage 16 phenotype of mam^ΔC

(Zhang et al., 2011)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^sim.P3.7, spi^s.UAS has midline glial cell | embryonic stage 13 | increased number phenotype, non-enhanceable by mam^ΔC

(Zhang et al., 2011)

Scer\GAL4^sim.P3.7, spi^s.UAS has midline glial cell | embryonic stage 16 | increased number phenotype, non-enhanceable by mam^ΔC

(Zhang et al., 2011)

NOT suppressed by

Statement

Reference

N^{Intra1768.UAS.Tag:FLAG}, Scer\GAL4^sim.P3.7 has midline glial cell | increased number | embryonic stage 16 phenotype, non-suppressible by mam^ΔC

(Zhang et al., 2011)

Scer\GAL4^sim.P3.7, spi^s.UAS has midline glial cell | increased number | embryonic stage 13 phenotype, non-suppressible by mam^ΔC

(Zhang et al., 2011)

Scer\GAL4^sim.P3.7, spi^s.UAS has midline glial cell | increased number | embryonic stage 16 phenotype, non-suppressible by mam^ΔC

(Zhang et al., 2011)

Egfr^U.A887T.UAS, Scer\GAL4^sim.P3.7 has midline glial cell | increased number | embryonic stage 16 phenotype, non-suppressible by Rat\Neu^KDYD.UAS, Scer\GAL4^sim.P3.7

(Settle et al., 2003)

Enhancer of

Statement

Reference

Scer\GAL4^sim.P3.7/Mmus\Sox2^{ΔHMG.UAS.EYFP,Tag:MYC} is an enhancer of larval central nervous system phenotype of D^r72/Df(3L)fz-GS1a

(Shen et al., 2013)

Mmus\Sox2^{EnR.UAS.Tag:MYC}/Scer\GAL4^sim.P3.7 is an enhancer of larval central nervous system phenotype of D^r72/Df(3L)fz-GS1a

(Shen et al., 2013)

Scer\GAL4^sim.P3.7/N^{Intra1768.UAS.Tag:FLAG} is an enhancer of midline glial cell | embryonic stage 16 | increased number phenotype of mam^ΔC

(Zhang et al., 2011)

NOT Enhancer of

Statement

Reference

spi^s.UAS/Scer\GAL4^sim.P3.7 is a non-enhancer of midline glial cell | embryonic stage 16 | increased number phenotype of mam^ΔC

(Zhang et al., 2011)

Suppressor of

Statement

Reference

Mmus\Sox2^UAS.EYFP/Scer\GAL4^sim.P3.7 is a suppressor of larval central nervous system phenotype of D^r72/Df(3L)fz-GS1a

(Shen et al., 2013)

dlp^UAS.cBa/Scer\GAL4^sim.P3.7 is a suppressor | partially of fascicle | ectopic | embryonic stage phenotype of Sdc²³

(Chanana et al., 2009)

Scer\GAL4^sim.P3.7/Egfr^Elp.UAS is a suppressor | partially of larval ventral midline glial cell phenotype of jing³/Df(2R)ST1

(Sonnenfeld et al., 2004)

Scer\GAL4^sim.P3.7/Egfr^Elp.UAS is a suppressor of larval ventral midline glial cell phenotype of jing³

(Sonnenfeld et al., 2004)

Ras85D^V12.UAS/Scer\GAL4^sim.P3.7 is a suppressor of larval ventral midline glial cell phenotype of jing³

(Sonnenfeld et al., 2004)

NOT Suppressor of

Statement

Reference

spi^s.UAS/Scer\GAL4^sim.P3.7 is a non-suppressor of midline glial cell | increased number | embryonic stage 16 phenotype of mam^ΔC

(Zhang et al., 2011)

Rat\Neu^KDYD.UAS, Scer\GAL4^sim.P3.7 is a non-suppressor of midline glial cell | increased number | embryonic stage 16 phenotype of Egfr^U.A887T.UAS, Scer\GAL4^sim.P3.7

(Settle et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

bgm^UAS.Tag:FLAG/Scer\GAL4^sim.P3.7 rescues bgm¹

(Gordon et al., 2018)

sli^UAS.cKa/Scer\GAL4^sim.P3.7 rescues sli²

(Orgogozo et al., 2004)

Partially rescues

NetA^UAS.cHa/Scer\GAL4^sim.P3.7 partially rescues Df(1)NetAB^Δ

(Newquist et al., 2013)

Scer\GAL4^sim.P3.7/NetB^UAS.cHa partially rescues Df(1)NetAB^Δ

(Newquist et al., 2013)

comm^UAS.cKa/Scer\GAL4^sim.P3.7 partially rescues comm¹

(Georgiou and Tear, 2002)

sli^UAS.cKa/Scer\GAL4^sim.P3.7 partially rescues sli²

(Kramer et al., 2001)

sli^UAS.cKa/Scer\GAL4^sim.P3.7 partially rescues sli²

(Kramer et al., 2001)

Scer\GAL4^sim.P3.7/sli^UAS.cBa partially rescues sli²

(Battye et al., 1999)

rho^UAS.cXa/Scer\GAL4^sim.P3.7 partially rescues rho^7M43

(Xiao et al., 1996)

Fails to rescue

NetA^UAS.cHa/Scer\GAL4^sim.P3.7 fails to rescue Df(1)NetAB^Δ

(Newquist et al., 2013)

Sdc^{PA.UAS.Tag:FLAG,GFP}/Scer\GAL4^sim.P3.7 fails to rescue Sdc²³

(Chanana et al., 2009)

β-Spec^UAS.Tag:MYC/Scer\GAL4^sim.P3.7 fails to rescue β-Spec^em6

(Garbe et al., 2007)

Delta^UAS.cDa/Scer\GAL4^sim.P3.7 fails to rescue Delta^vi1/Delta^6B

(Crowner et al., 2003)

comm^UAS.cKa/Scer\GAL4^sim.P3.7 fails to rescue comm^Δe39

(Georgiou and Tear, 2002)

rho^UAS.cXa/Scer\GAL4^sim.P3.7 fails to rescue rho^PΔ38

(Lanoue and Jacobs, 1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

26784

w^*; P{GAL4-sim.3.7}2, P{UAS-GFP.btau}2

9150

w^*; P{GAL4-sim.3.7}2/CyO; P{GAL4-sim.3.7}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

GAL4^sim.P3.7

Scer\GAL4^sim.P3.7

Name Synonyms

Secondary FlyBase IDs

References (52)

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