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General Information
Symbol
Dmel\dlpUAS.cBa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Baeg
FlyBase ID
FBal0121805
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-dlp
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of full length dlp (an XhoI-XbaI fragment).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of dlpScer\UAS.cBa under the control of Scer\GAL4how-24B leads to larval neuromuscular junctions exhibiting significantly increased branch number and increased type I synaptic bouton number, significantly increased amplitude of evoked junctional currents, as compared to controls.

Expression of dlpScer\UAS.cBa under the control of Scer\GAL4nub-AC-62 results in a very slight reduction in wing margin bristles compared to wild type. Wing blades are largely of normal size and show no signs of scalloping at the margin.

Expression of dlpScer\UAS.cBa under the control of a postsynaptic Scer\GAL4 driver results in an increase in mean active zone area at the neuromuscular junction compared to wild type.

Expression of dlpScer\UAS.cBa under the control of either Scer\GAL4how-24B or Scer\GAL4elav.PU results in a reduction in bouton number at the neuromuscular junction compared to wild type.

Expression of dlpScer\UAS.cBa under the simultaneous control of both Scer\GAL4sca.PU and Scer\GAL4how-24B results in a reduction in bouton number at the neuromuscular junction compared to wild type.

Expression of dlpScer\UAS.cBa, under the control of both Scer\GAL4how-24B and Scer\GAL4G14, does not produce a SNa phenotype.

Expression of dlpScer\UAS.cBa, under the control of Scer\GAL4ap-md544, in a dlp1/+ background leads to bristle loss at the anterior wing margin.

Adults expressing dlpScer\UAS.cBa under the control of Scer\GAL4en-e16E have notched wings.

Expression of dlpScer\UAS.cBa under the control of Scer\GAL4C96 results in severe wing margin defects and loss of sensory bristles.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Suppressed by
Enhancer of
NOT Enhancer of
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

The increase in average cell number in the stalks between egg chambers which is seen in Mmp2Y53N/Mmp2W307stop females after being shifted to the restrictive temperature is enhanced if they are also expressing dlpScer\UAS.cBa under the control of Scer\GAL4C587.

Expression of dlpScer\UAS.cBa does not enhance the reduction in adult wing size seen when fz2GPI.Scer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4nub-AC-62.

Scer\GAL4elav.PU-, Scer\GAL4sim.P3.7- or Scer\GAL4repo.PU-mediated expression of dlpScer\UAS.cBa partially rescues the Sdc23 ventral midline fascicle cross-over phenotype.

Expression of dlpScer\UAS.cBa under the control of Scer\GAL4how-24B does not rescue the reduction in bouton number at the neuromuscular junction seen in homozygous Sdc10608 larvae.

Larvae co-expressing SdcScer\UAS.cJa and dlpScer\UAS.cBa under the control of Scer\GAL4how-24B show a reduction in bouton number at the neuromuscular junction compared to wild type, as occurs in larvae expressing dlpScer\UAS.cBa alone under the control of Scer\GAL4how-24B.

Expression of dlpScer\UAS.cBa under the control of Scer\GAL4sli.PS fails to rescue the axon midline crossing phenotype seen in Df(2R)48 embryos in which Sara function has been rescued by SaraUbi.PJ. Expression of dlpScer\UAS.cBa under the control of Scer\GAL4elav.PLu significantly rescues the axon midline crossing phenotype seen in Df(2R)48 embryos in which Sara function has been rescued by SaraUbi.PJ.

Co-expression of wgl-12.Scer\UAS or armS10.Scer\UAS.T:Hsap\MYC can rescue both the wing notching phenotype and loss of sensory bristles caused by expression of dlpScer\UAS.cBa under the control of Scer\GAL4C96. In addition, ectopic margin bristles close to the wing margin are seen in these flies.

Xenogenetic Interactions
Statement
Reference

Expression of dlpScer\UAS.cBa strongly enhances the wing scalloping and bristle loss phenotypes seen when Drer\wif1Scer\UAS.cAa is expressed under the control of Scer\GAL4nub-AC-62.

Expression of dlpScer\UAS.cBa synergistically enhances the wing margin scalloping and wing blade reduction seen when Drer\wif1pVal.Scer\UAS.T:SV5\V5 is expressed under the control of Scer\GAL4nub-AC-62. The loss of wing margin bristles is also enhanced, consistent with the two transgenes having an additive effect.

dlpScer\UAS.cBa additively enhances the loss of wing margin bristles seen when Drer\wif1ΔEGF.Scer\UAS.T:SV5\V5 is expressed under the control of Scer\GAL4nub-AC-62. The wing margin defects seen in Drer\wif1ΔEGF.Scer\UAS.T:SV5\V5 are not enhanced by dlpScer\UAS.cBa.

Expression of dlpScer\UAS.cBa synergistically enhances the wing scalloping and bristle loss phenotypes seen when shf::Drer\wif1EDF-WIF.Scer\UAS.T:SV5\V5 is expressed under the control of Scer\GAL4nub-AC-62. Wing margin bristles and the first longitudinal vein are almost completely eliminated and wing size is further reduced.

Complementation and Rescue Data
Partially rescues

dlpUAS.cBa/Scer\GAL4[-] partially rescues dlp1/Df(3L)fz-D21

Comments

Expression of dlpScer\UAS.cBa under the control of Scer\GAL4prd.RG1 restores cuticle patterning in entire segments in a non-cell-autonomous manner in dlpA187 embryos derived from dlpA187 female germline clones.

Expression of dlpScer\UAS.cBa without a driver is sufficient to rescue the eye phenotype, but not the wing phenotype of dlp1/Df(3L)fz-D21 mutants.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
dlpScer\UAS.cBa
dlpUAS.cBa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Baeg
Secondary FlyBase IDs
    References (24)