Expression of wgUAS.cHa.Tag:HA under the control of Scer\GAL4rn-GAL4-5 (with tub-Gal80[ts] restricting expression to third instar larvae) neither in combination with egrUAS.cUa nor in CtBP87De-10/+ background leads to ectopic wings compared to controls.
Restricted expression of wgUAS.cHa.Tag:HA to second but not third instar larvae (under the control of Scer\GAL4ptc.PU and tub-Gal80[ts]) leads to ectopic wing pouches in wing discs in CtBP87De-10/+ background compared to controls.
The increased number of intestinal progenitor cells in mitosis characteristic for adult flies expressing wgScer\UAS.cHa.T:Ivir\HA1 driven by Scer\GAL4esg-NP7397 (combined with tub-Gal80[ts] for temporal control) is suppressed by co-expression of RetGD45.
The reduction in total cell number per clone and reduction in number of Dl-positive intestinal stem cells per clone seen in MARCM clones in the intestine of lin-28Δ1 adults is not rescued by expression of wgScer\UAS.cHa.T:Ivir\HA1 under the control of Scer\GAL4tub.PU.
Co-expression of Mmp2Scer\UAS.cPa has no effect on the increase in stalk cell numbers seen in females expressing wgScer\UAS.cHa.T:Ivir\HA1 under the control of Scer\GAL4C587 (using the temperature sensitive Scer\GAL80ts.αTub84B allele to limit expression to the adult stage).