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General Information
Symbol
Dmel\wgUAS.cHa.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0065588
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-wg, UAS-wg-HA, UAS-wg+
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

The FRT cassette has been excised, juxtaposing the UASt regulatory sequences and wg coding sequences tagged at amino acid residue 111 with Tag:HA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
ameliorates  obesity
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of wgUAS.cHa.Tag:HA under the control of Scer\GAL4nSyb.PS significantly increases bouton number at the neuromuscular junction of muscle 4, compared to controls, with no effect on bouton size.

Expression of wgUAS.cHa.Tag:HA under the control of Scer\GAL4rn-GAL4-5 (with tub-Gal80[ts] restricting expression to third instar larvae) does not lead to ectopic wings compared to controls.

Restricted expression of wgUAS.cHa.Tag:HA to second but not third instar larvae (under the control of Scer\GAL4ptc.PU and tub-Gal80[ts]) leads to ectopic wings compared to controls.

Adult flies expressing wgScer\UAS.cHa.T:Ivir\HA1 driven by Scer\GAL4esg-NP7397 combined with tub-Gal80[ts] (for temporal control) display increase in midgut stem cell proliferation (assessed by number of pH3-positive cells).

Expression of wgScer\UAS.cHa.T:Ivir\HA1 under the Scer\GAL4svp.PT driver does not change ostia cell shape.

Adults expressing wgUAS.cHa.Tag:HA under the control of Scer\GAL4Mef2.PR have a reduced abdominal fat body with smaller lipid droplets than controls.

Third instar larvae expressing wgUAS.cHa.Tag:HA under the control of Scer\GAL4DcG have a severely smaller fat body in the abdominal region.

Expression of wgScer\UAS.cHa.T:Ivir\HA1 under the control of Scer\GAL4bab1-Gal4 results in increased numbers of stalk cells between egg chambers.

Females expressing wgScer\UAS.cHa.T:Ivir\HA1 under the control of Scer\GAL4C587 (using the temperature sensitive Scer\GAL80ts.αTub84B allele to limit expression to the adult stage) show an increase in the average number of cells in the interfollicle stalks compared to wild type.

Ectopic expression of wgUAS.cHa.Tag:HA under the control of Scer\GAL4btl.PS (in combination with a Gal80[ts] transgene to overcome embryonic and larval lethality) in spiracular branch (SB) growth zones 1 and 2, leads to proliferation of the SB cells and expansion of SB growth zone 2. The wgUAS.cHa.Tag:HA expressing SB zone 1 cells resemble zone 2 cells; they do not undergo endoreplication as indicated by their small nuclei, but instead remain in an undifferentiated state.

Expression of wgScer\UAS.cHa.T:Ivir\HA1 in post-mitotic eye cells under the control of Scer\GAL4GMR.PF results in smaller eyes that have a 'glossy' appearance. Analysis of the eye nipple arrays in these overexpression mutants reveals a catastrophic loss of nipples, with the remaining nanostructures randomly spaced with large gap areas.

Overexpression of wgScer\UAS.cHa.T:Ivir\HA1 using Scer\GAL4dpp.blk1 decreases the nuclear size of distal salivary gland cells. Thus, the normal difference in size between proximal and distal salivary gland cells is small in these animals.

Very few nuclei incorporate BrdU in wgScer\UAS.cHa.T:Ivir\HA1, Scer\GAL4dpp.blk1 salivary glands.

Expression of wgScer\UAS.cHa.T:Ivir\HA1 in the larvae under the control of Scer\GAL4Act5C.Switch.PR results in lethality. Both male and female life-span are reduced when Ras85DV12.Scer\UAS is expressed in adult flies.

Expression of wgScer\UAS.cHa.T:Ivir\HA1 under the control of Scer\GAL4dome-PG14 blocks differentiation of hemocyte precursors in the lymph gland.

Expression of wgScer\UAS.cHa.T:Ivir\HA1 under the control of Scer\GAL4Antp-10 results in an increase in the number of cells of the posterior signalling center of the lymph gland.

Expression of wgScer\UAS.cHa.T:Ivir\HA1 under the control of Scer\GAL4lz-gal4 results in an increase in the number of crystal cells.

Expression of wgScer\UAS.cHa.T:Ivir\HA1 under the control of Scer\GAL4elav.PH in clones induced by FLP/FRT mediated recombination (to activate the Scer\GAL4elav.PH driver) results in an increase in the number of type 1b boutons at muscle 13 compared to wild type.

Overexpression of wgScer\UAS.cHa.T:Ivir\HA1 starting from early larval stages driven by Scer\GAL4byn-Gal4 in combination with Scer\GAL80ts.αTub84B results in a marked expansion of the size of the hindgut proliferation zone in late-stage larvae and in newly eclosed adult flies. A similar effect can be achieved by expressing wgScer\UAS.cHa.T:Ivir\HA1 exclusively during the adult stage. At higher expression levels of wgScer\UAS.cHa.T:Ivir\HA1 (achieved by increasing the temperature for the experiments) the proportion of spindle cell zone cells in the posterior intestine is increased compared to wild-type. Overexpression of wgScer\UAS.cHa.T:Ivir\HA1 at somewhat lower temperatures results in a less extreme expansion of the hindgut proliferation zone and there is an apparent increase in round cell zone cells.

Embryos derived from females homozygous for wgScer\UAS.cHa.T:Ivir\HA1 crossed to males homozygous for Scer\GAL4e22c have a naked cuticle phenotype.

When wgScer\UAS.cHa.T:Ivir\HA1 is driven by Scer\GAL4C380 or Scer\GAL4C164, a slight increase in the number of synaptic boutons is seen.

Expression of wgScer\UAS.cHa.T:Ivir\HA1 using Scer\GAL4e22c in wgl-17 embryos causes all ventral epidermal cells to secrete naked cuticle. wgl-17 embryos expressing wgScer\UAS.cHa.T:Ivir\HA1 under the control of Scer\GAL4wg.PM show full rescue of the ventral cuticle pattern, although the embryos are slightly distorted as the dorsal pattern elements are not rescued.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT Enhanced by
Statement
Reference

Scer\GAL4ptc.PU, wgUAS.cHa.Tag:HA has wing | ectopic phenotype, non-enhanceable by CtBP[+]/CtBP87De-10

Suppressed by
NOT suppressed by
NOT Enhancer of
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

Expression of wgUAS.cHa.Tag:HA under the control of Scer\GAL4rn-GAL4-5 (with tub-Gal80[ts] restricting expression to third instar larvae) neither in combination with egrUAS.cUa nor in CtBP87De-10/+ background leads to ectopic wings compared to controls.

Restricted expression of wgUAS.cHa.Tag:HA to second but not third instar larvae (under the control of Scer\GAL4ptc.PU and tub-Gal80[ts]) leads to ectopic wing pouches in wing discs in CtBP87De-10/+ background compared to controls.

The increased number of intestinal progenitor cells in mitosis characteristic for adult flies expressing wgScer\UAS.cHa.T:Ivir\HA1 driven by Scer\GAL4esg-NP7397 (combined with tub-Gal80[ts] for temporal control) is suppressed by co-expression of RetGD45.

The reduction in total cell number per clone and reduction in number of Dl-positive intestinal stem cells per clone seen in MARCM clones in the intestine of lin-28Δ1 adults is not rescued by expression of wgScer\UAS.cHa.T:Ivir\HA1 under the control of Scer\GAL4tub.PU.

Co-expression of Mmp2Scer\UAS.cPa has no effect on the increase in stalk cell numbers seen in females expressing wgScer\UAS.cHa.T:Ivir\HA1 under the control of Scer\GAL4C587 (using the temperature sensitive Scer\GAL80ts.αTub84B allele to limit expression to the adult stage).

Co-expression of sspScer\UAS.cTa and wgScer\UAS.cHa.T:Ivir\HA1 using Scer\GAL4dpp.blk1 suppresses the heterogeneity of salivary gland nuclear size seen when sspScer\UAS.cTa is expressed alone.

The naked cuticle phenotype of embryos derived from females homozygous for wgScer\UAS.cHa.T:Ivir\HA1 crossed to males homozygous for Scer\GAL4e22c is not affected by eRF1unspecified or Elfunspecified.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

wgScer\UAS.cHa.T:Ivir\HA1 completely rescues the neuromuscular junction expansion phenotype seen in wgl-12 animals, when driven by Scer\GAL4C380.

Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
Comments
Comments

Exists in clones, not reported as being germ-line transmitted.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
wgScer\UAS.cHa.T:Ivir\HA1
wgUAS.cHa.T:HA1
wgUAS.cHa.Tag:HA
Name Synonyms
Secondary FlyBase IDs
    References (28)