FlyBase Allele Report: Scer\GAL4[wg.PM]

General Information

Symbol

Scer\GAL4^wg.PM

Species

S. cerevisiae

Name

wingless promoter construct of Martinez-Arias

FlyBase ID

FBal0065090

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{GAL4-wg.M}

Also Known As

wg-GAL4, wgGal4, wingless-GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Gieseler et al., 1997.10.13)

Progenitor genotype

Carried in construct

P{GAL4-wg.M}

Cytology

Description

7.8kb of 5' untranslated control region of wg regulates expression of a GAL4 driver.

(Gieseler et al., 1997.10.13)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

proventriculus | restricted

Comment: foregut/midgut junction

(Park and Kwon, 2011)

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Gieseler et al., 1997.10.13)

organism | segment polarity expression pattern

(Glise and Noselli, 1997)

adult stage

proventriculus | restricted

(Singh et al., 2011)

Additional Information

Statement

Reference

Drives embryonic expression in a segment polarity expression pattern; does not drive expression in imaginal discs.

(FlyBase Curators, 2017)

The wg reporter is expressed in the AvicGFP^Stat92E.10X domain at the junction of the foregut and midgut.

(Park and Kwon, 2011)

Expression observed in embryos; no expression in imaginal discs.

(Gieseler et al., 1997.10.13)

Marker for

Reflects expression of

(Park and Kwon, 2011, Gieseler et al., 1997.10.13, Glise and Noselli, 1997)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with rpr^UAS.cZa

(Morris et al., 2006)

visible, with Cul5^EP3390

(Ayyub et al., 2005)

visible, with E2f1^UAS.cNa

(Morris et al., 2006)

Phenotype Manifest In

abdominal ventral acute muscle 1 founder cell, with shi^K44A.UAS

(Cox et al., 2005)

antenna | increased number, with Cul5^EP3390

(Ayyub et al., 2005)

denticle | cell autonomous | ectopic, with SoxN^UAS.Venus

(Overton et al., 2007)

denticle | ectopic, with ovo^UAS.cPa

(Payre et al., 1999)

denticle | ectopic, with Rac1^V12.UAS

(Jones and Bejsovec, 2005)

denticle | increased number, with ovo^svb.UAS

(Chanut-Delalande et al., 2006)

embryonic/first instar larval cuticle, with pan^ΔN.UAS

(Moline et al., 1999)

embryonic epidermis, with ovo^UAS.cPa

(Payre et al., 1999)

embryonic mandibular segment, with Diap1^UAS.cHa

(Lohmann et al., 2002)

embryonic maxillary segment, with Diap1^UAS.cHa

(Lohmann et al., 2002)

embryonic procephalic segment, with Diap1^UAS.cHa

(Lohmann et al., 2002)

germline cell, with ttv^UAS.Tag:MYC

(Deshpande et al., 2007)

myoblast, with Df(1)w67k30, kirre^UAS.cRa

(Ruiz-Gomez et al., 2000)

ventral denticle belt, with ovo^UAS.A.GFP

(Kondo et al., 2010)

ventral denticle belt, with ovo^UAS.cPa

(Payre et al., 1999)

ventral denticle belt, with pan^ΔN.UAS

(Moline et al., 1999)

ventral denticle belt | ectopic, with ovo^UAS.B.GFP

(Kondo et al., 2010)

ventral denticle belt | ectopic, with ovo^UAS.svb.GFP

(Kondo et al., 2010)

wing, with Cul5^EP3390

(Ayyub et al., 2005)

wing blade, with E2f1^UAS.cNa

(Morris et al., 2006)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^wg.PM, ovo^UAS.svb.GFP has ventral denticle belt | ectopic phenotype, suppressible by tal^unspecified

(Kondo et al., 2010)

NOT suppressed by

Statement

Reference

Scer\GAL4^wg.PM, ovo^UAS.B.GFP has ventral denticle belt | ectopic phenotype, non-suppressible by tal^unspecified

(Kondo et al., 2010)

Suppressor of

Statement

Reference

Nrt::wg^{Scer\FRT-.UAS.Tag:HA}/Scer\GAL4^wg.PM is a suppressor of abdominal ventral acute muscle 1 founder cell phenotype of wg^l-17

(Cox et al., 2005)

Nrt::wg^{Scer\FRT-.UAS.Tag:HA}/Scer\GAL4^wg.PM is a suppressor of abdominal ventral acute muscle 2 founder cell phenotype of wg^l-17

(Cox et al., 2005)

Nrt::wg^{Scer\FRT-.UAS.Tag:HA}/Scer\GAL4^wg.PM is a suppressor of abdominal ventral acute muscle 3 founder cell phenotype of wg^l-17

(Cox et al., 2005)

Nrt::wg^{Scer\FRT-.UAS.Tag:HA}/Scer\GAL4^wg.PM is a suppressor of abdominal lateral oblique muscle 1 founder cell phenotype of wg^l-17

(Cox et al., 2005)

rpr^UAS.C/Scer\GAL4^wg.PM is a suppressor of embryonic mandibular segment phenotype of Dfd^unspecified

(Lohmann et al., 2002)

rpr^UAS.C/Scer\GAL4^wg.PM is a suppressor of embryonic maxillary segment phenotype of Dfd^unspecified

(Lohmann et al., 2002)

klar^UAS.Tag:MYC/Scer\GAL4^wg.PM is a suppressor of presumptive embryonic salivary gland phenotype of hkb²

(Myat and Andrew, 2002)

Wnt6^UAS.cSa/Scer\GAL4^wg.PM is a suppressor | partially of tracheal dorsal trunk primordium phenotype of Wnt2^unspecified, wg^l-17

(Llimargas and Lawrence, 2001)

wntD^UAS.cSa/Scer\GAL4^wg.PM is a suppressor | partially of tracheal dorsal trunk primordium phenotype of Wnt2^unspecified, wg^l-17

(Llimargas and Lawrence, 2001)

Nrt::wg^UAS.Tag:HA/Scer\GAL4^wg.PM is a suppressor of phenotype of wg^l-17

(Pfeiffer et al., 2000)

NOT Suppressor of

Statement

Reference

Wnt10^UAS.cSa/Scer\GAL4^wg.PM is a non-suppressor of tracheal dorsal trunk primordium phenotype of Wnt2^unspecified, wg^l-17

(Llimargas and Lawrence, 2001)

Wnt4^UAS.cSa/Scer\GAL4^wg.PM is a non-suppressor of tracheal dorsal trunk primordium phenotype of Wnt2^unspecified, wg^l-17

(Llimargas and Lawrence, 2001)

Wnt5^UAS.cSa/Scer\GAL4^wg.PM is a non-suppressor of tracheal dorsal trunk primordium phenotype of Wnt2^unspecified, wg^l-17

(Llimargas and Lawrence, 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

zip^UAS.mGFP6/Scer\GAL4^wg.PM rescues zip³

(Okumura et al., 2010)

zip^UAS.mGFP6/Scer\GAL4^wg.PM rescues zip⁴

(Okumura et al., 2010)

kirre^{Δ610-959.UAS.Tag:FLAG}/Scer\GAL4^wg.PM rescues Df(1)w67k30

(Menon et al., 2005)

ttv^UAS.Tag:MYC/Scer\GAL4^wg.PM rescues ttv^00681b

(Gallet et al., 2003)

wg^UAS.GFP/Scer\GAL4^wg.PM rescues wg^l-17

(Pfeiffer et al., 2002)

fu^UAS.cTa/Scer\GAL4^wg.PM rescues fu⁴¹

(Therond et al., 1999)

wg^{UAS.cHa.Tag:HA}/Scer\GAL4^wg.PM rescues wg^l-17

(Hays et al., 1997)

wg^UAS.cHa/Scer\GAL4^wg.PM rescues wg^l-17

(Hays et al., 1997)

Partially rescues

mid^UAS.cGa/Scer\GAL4^wg.PM partially rescues Df(2L)Exel6012

(Gaziova and Bhat, 2009)

mid^UAS.cGa/Scer\GAL4^wg.PM partially rescues mid^los1

(Gaziova and Bhat, 2009)

Rho1^UAS.cHa/Scer\GAL4^wg.PM partially rescues Rho1^1B

(Xu et al., 2008)

wg^UAS.HRP/Scer\GAL4^wg.PM partially rescues wg^l-17

(Dubois et al., 2001)

wg^wg.3'UTR.UAS/Scer\GAL4^wg.PM partially rescues wg^l-17

(Simmonds et al., 2001)

wg^{SV40.3'UTR.UAS}/Scer\GAL4^wg.PM partially rescues wg^l-17

(Simmonds et al., 2001)

lin^UAS.cHa/Scer\GAL4^wg.PM partially rescues lin^G2/lin²

(Hatini et al., 2000)

wg^{PE2.UAS.Tag:HA}/Scer\GAL4^wg.PM partially rescues wg^l-17

(Hays et al., 1997)

wg^PE4.UAS/Scer\GAL4^wg.PM partially rescues wg^l-17

(Hays et al., 1997)

wg^PE2.UAS/Scer\GAL4^wg.PM partially rescues wg^l-17

(Hays et al., 1997)

wg^{PE4.UAS.Tag:HA}/Scer\GAL4^wg.PM partially rescues wg^l-17

(Hays et al., 1997)

Fails to rescue

zip^UAS.mGFP6/Scer\GAL4^wg.PM fails to rescue zip³

(Okumura et al., 2010)

kirre^{Δ586-959.UAS.Tag:FLAG}/Scer\GAL4^wg.PM fails to rescue Df(1)w67k30

(Menon et al., 2005)

wg^{Ppa.3'UTR.UAS}/Scer\GAL4^wg.PM fails to rescue wg^l-17

(Simmonds et al., 2001)

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

4918

107949

101852

w^* ; P{GAL4-wg.M}MA1

Notes on Origin

Discoverer

Comments

wg DNA provides all functions for the embryonic pattern and contains only a very small and not clear pattern from the adult.

(Gieseler et al., 1997.10.13)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

GAL4^wg.PM

Scer\GAL4^wg.PM

Name Synonyms

wingless promoter construct of Martinez-Arias

(Gieseler et al., 1997.10.13)

Secondary FlyBase IDs

References (53)

Report Sections

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