FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\zip3
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General Information
Symbol
Dmel\zip3
Species
D. melanogaster
Name
FlyBase ID
FBal0263483
Feature type
allele
Associated gene
Dmel\zip
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Okumura et al., 2010)
Progenitor genotype
Cytology
Description

Amino acid replacement: Q1076term.

(Okumura et al., 2010)

The mutation results in a truncated protein that lacks part of the coiled coil domain.

(Okumura et al., 2010)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:24,994,920..24,994,920 [-]
Nucleotide change:

C24994920T

Amino acid change:

Q1076term | zip-PA; Q1031term | zip-PB; Q991term | zip-PC; Q1036term | zip-PD; Q991term | zip-PE; Q999term | zip-PF; Q991term | zip-PG; Q999term | zip-PH; Q1044term | zip-PI

Reported amino acid change:

Q1076term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Okumura et al., 2010)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal axis specification | embryonic stage 15 (with Df(2R)gsb)

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 15 (with zip1)

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 15 (with zip2)

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 15 (with zip4)

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 15 | recessive

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 16 (with Df(2R)gsb)

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 16 (with zip1)

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 16 (with zip2)

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 16 (with zip4)

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 16 | recessive

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 17 (with Df(2R)gsb)

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 17 (with zip1)

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 17 (with zip2)

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 17 (with zip4)

      (Okumura et al., 2010)

      abnormal axis specification | embryonic stage 17 | recessive

      (Okumura et al., 2010)

      lethal - all die before end of larval stage

      (Okumura et al., 2010)

      lethal - all die before end of larval stage (with Df(2R)gsb)

      (Okumura et al., 2010)

      lethal - all die before end of larval stage (with zip1)

      (Okumura et al., 2010)

      lethal - all die before end of larval stage (with zip2)

      (Okumura et al., 2010)

      lethal - all die before end of larval stage (with zip4)

      (Okumura et al., 2010)

      some die during larval stage

      (Okumura et al., 2010)

      some die during larval stage (with Df(2R)gsb)

      (Okumura et al., 2010)

      some die during larval stage (with zip1)

      (Okumura et al., 2010)

      some die during larval stage (with zip2)

      (Okumura et al., 2010)

      some die during larval stage (with zip4)

      (Okumura et al., 2010)
      Phenotype Manifest In

      embryonic/larval anterior midgut | embryonic stage 15 (with Df(2R)gsb)

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 15 (with zip1)

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 15 (with zip2)

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 15 (with zip4)

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 16 (with Df(2R)gsb)

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 16 (with zip1)

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 16 (with zip2)

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 16 (with zip4)

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 17 (with Df(2R)gsb)

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 17 (with zip1)

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 17 (with zip2)

      (Okumura et al., 2010)

      embryonic/larval anterior midgut | embryonic stage 17 (with zip4)

      (Okumura et al., 2010)

      embryonic esophagus | embryonic stage 17

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 15 (with Df(2R)gsb)

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 15 (with zip1)

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 15 (with zip2)

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 15 (with zip4)

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 16 (with Df(2R)gsb)

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 16 (with zip1)

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 16 (with zip2)

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 16 (with zip4)

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 17 (with Df(2R)gsb)

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 17 (with zip1)

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 17 (with zip2)

      (Okumura et al., 2010)

      embryonic proventriculus | embryonic stage 17 (with zip4)

      (Okumura et al., 2010)
      Detailed Description
      Statement
      Reference

      zip3 homozygous mutants show laterality defects in the proventriculus and anterior midgut. The laterality of the other parts of the embryonic gut, including the hindgut and the posterior part of the midgut is normal in these mutants. The proventriculus and the anterior midgut do not rotate (as in wild-type) in these mutants at stages 15 to 17.

      zip3 heterozygotes appear wild-type.

      zip3/zip4 embryos show laterality defects in the proventriculus and anterior midgut. The laterality of the other parts of the embryonic gut, including the hindgut and the posterior part of the midgut is normal in these mutants. The proventriculus and the anterior midgut do not rotate (as in wild-type) in these mutants at stages 15 to 17.

      zip3/zip1 embryos show laterality defects in the proventriculus and anterior midgut. The laterality of the other parts of the embryonic gut, including the hindgut and the posterior part of the midgut is normal in these mutants. The proventriculus and the anterior midgut do not rotate (as in wild-type) in these mutants at stages 15 to 17.

      zip3/zip2 embryos show laterality defects in the proventriculus and anterior midgut. The laterality of the other parts of the embryonic gut, including the hindgut and the posterior part of the midgut is normal in these mutants. The proventriculus and the anterior midgut do not rotate (as in wild-type) in these mutants at stages 15 to 17.

      zip3/Df(2R)gsb embryos show laterality defects in the proventriculus and anterior midgut. The laterality of the other parts of the embryonic gut, including the hindgut and the posterior part of the midgut is normal in these mutants. The proventriculus and the anterior midgut do not rotate (as in wild-type) in these mutants at stages 15 to 17.

      Approximately 20% of zip3 stage 17 embryos show left-right inversion of looping of the esophagus.

      zip3 mutants exhibit dorsal closure defects.

      Differentiation and development of the trunk visceral mesoderm appears normal in stage 12 zip3 homozygous embryos.

      The circular visceral muscle appears to be correctly differentiated and migrates normally in stage 16 zip3 homozygotes.

      Examination of the actin cytoskeleton through expression of MoeScer\UAS.T:Avic\GFP under the control of Scer\GAL4NetB-NP1522) shows that the structure and closure of the midgut in zip3 stage 15 and 16 embryos is comparable to controls.

      The right side specific rearrangement of the circular visceral muscle cells is abolished in zip3 stage 15-16 embryos.

      The rotation of the ventral midline of the circular visceral muscle cells relative to the midline of the embryo that is seen in wild type does not occur in stage 16 zip3 embryos.

      The posterior migration of the ventral anterior midgut epithelium (examined using MoeScer\UAS.T:Avic\GFP and Disc\RFPDsRedT4.Scer\UAS.T:nls5 under the control of the Scer\GAL448Y driver) occurs normally in early stage 16 zip3 mutants compared to controls. The movement of the anterior midgut that normally occurs at late stage 16 is disrupted in zip3 homozygotes with the posterior movement, rightward movement of the anterior region and leftward movement of the posterior region of the anterior midgut all affected.

      (Okumura et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Enhancer of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      A chic221 heterozygous background enhances the loss of rotation in the proventriculus and anterior midgut seen in zip3 homozygotes.

      A zip3 heterozygous background enhances the loss of rotation in the proventriculus and anterior midgut seen in sqhAX3/Y mutants.

      (Okumura et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      zip3 is rescued by Scer\GAL4da.G32/zipUAS.mGFP6

      (Okumura et al., 2010)

      zip3 is rescued by zipUAS.mGFP6/Scer\GAL4wg.PM

      (Okumura et al., 2010)
      Partially rescued by

      zip3 is partially rescued by Scer\GAL4how-24B/zipUAS.mGFP6

      (Okumura et al., 2010)

      zip3 is partially rescued by zipUAS.mGFP6/Scer\GAL4NP3392

      (Okumura et al., 2010)

      zip3 is partially rescued by zipUAS.mGFP6/Scer\GAL448Y

      (Okumura et al., 2010)

      zip3 is partially rescued by Scer\GAL4NP1522/zipUAS.mGFP6

      (Okumura et al., 2010)
      Not rescued by

      zip3 is not rescued by Scer\GAL4NP221/zipUAS.mGFP6

      (Okumura et al., 2010)

      zip3 is not rescued by Scer\GAL4tey-5053A/zipUAS.mGFP6

      (Okumura et al., 2010)

      zip3 is not rescued by Scer\GAL4NP5021/zipUAS.mGFP6

      (Okumura et al., 2010)

      zip3 is not rescued by Scer\GAL4drm.7.1/zipUAS.mGFP6

      (Okumura et al., 2010)

      zip3 is not rescued by Scer\GAL4byn-Gal4/zipUAS.mGFP6

      (Okumura et al., 2010)

      zip3 is not rescued by Scer\GAL4elav-C155/zipUAS.mGFP6

      (Okumura et al., 2010)

      zip3 is not rescued by zipUAS.mGFP6/Scer\GAL4wg.PM

      (Okumura et al., 2010)
      Comments

      zipScer\UAS.T:Avic\GFP driven by Scer\GAL4da.G32 rescues the loss of rotation in the proventriculus and anterior midgut seen in zip3 homozygotes.

      zipScer\UAS.T:Avic\GFP driven by the circular visceral muscle drivers Scer\GAL4how-24B, Scer\GAL4Gap1-NP3392, Scer\GAL448Y or Scer\GAL4NetB-NP1522 partially rescues the loss of rotation in the proventriculus and anterior midgut seen in zip3 homozygotes.

      zipScer\UAS.T:Avic\GFP driven by the longitudinal visceral muscle drivers Scer\GAL4NP221 or Scer\GAL4tey-5053A fails to rescue the loss of rotation in the proventriculus and anterior midgut seen in zip3 homozygotes.

      zipScer\UAS.T:Avic\GFP driven by the drivers Scer\GAL4NP5021, Scer\GAL4drm.7.1, Scer\GAL4byn-Gal4, Scer\GAL4elav-C155 or Scer\GAL4wg.PM fails to rescue the loss of rotation in the proventriculus and anterior midgut seen in zip3 homozygotes.

      Expression of zipScer\UAS.T:Avic\GFP driven by Scer\GAL4wg.PM suppresses the dorsal closure defects in zip3 homozygotes although the no rotation phenotype is not suppressed.

      Expression of zipScer\UAS.T:Avic\GFP continuously in a non-permissive temperature (32[o]C), under the control of Scer\GAL4da.G32 and Scer\GAL80ts.αTub84B, rescues the laterality defects found in zip3 mutants. The zip3 rotation defects are not rescued when the 1 hour shift to the non-permissive temperature occurs from stage 14 onwards. The temperature shift applied from 16 to 17 hours after egg laying (AEL), which corresponds to stage 12, leads to the most efficient rescue of the laterality defects associated with zip3 mutants. However, expression of zipScer\UAS.T:Avic\GFP after 22 hours AEL, corresponding to stage 14, does not rescue the laterality, 'no rotation' phenotype.

      zipScer\UAS.T:Avic\GFP driven by Scer\GAL4how-24B partially rescues the loss of side specific rearrangement in circular visceral muscle cells seen in zip3 homozygotes. The failure of the circular visceral muscle midline to rotate in relation to the midline of the embryo is also partially rescued.

      (Okumura et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      zip3
      (Okumura et al., 2010)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)