Co-expression of WScer\UAS.cZa and rprScer\UAS.cZa under the control of Scer\GAL4ptc-559.1 is lethal, but is viable when Scer\GAL80αTub84B.PL is expressed until the third instar larval stage. Robust cell death is seen in the wing disc 12 hours after Scer\GAL4 de-repression.
When larvae expressing WScer\UAS.cZa and rprScer\UAS.cZa under the control of Scer\GAL4ptc-559.1 are irradiated (and expression is repressed until 12 hours prior to irradiation using Scer\GAL80αTub84B.PL), less cell death is seen in the wing disc in comparison with irradiated controls. A heterozygous Df(3L)banΔ1 background has no effect on the amount of cell death, but when de-repression occurs 6 hours prior to irradiation (which results in a less dramatic reduction in cell death compared to 12 hours), heterozygous Df(3L)banΔ1 partially suppresses the level of protection seen when WScer\UAS.cZa and rprScer\UAS.cZa are expressed alone.
When wing discs containing WScer\UAS.cZa and rprScer\UAS.cZa expressing clones (repressed during early development using Scer\GAL80αTub84B.PL)) are irradiated, less cell death is seen outside of the clones in comparison with irradiated controls.
Expression of thScer\UAS.T:Hsap\MYC.cBa almost completely restores the eye phenotypes seen when rprScer\UAS.cZa is expressed under the control of Scer\GAL4GMR.PF at 29[o]C. Eye size is 98% of control and no roughness or other morphological abnormalities are observed. The loss of viability seen in flies expressing rprScer\UAS.cZa is rescued upon co-expression of thScer\UAS.T:Hsap\MYC.cBa.
Expression of thN117K.H283Y.Scer\UAS.T:Avic\GFP-YFP.Venus in the developing eye under the control of Scer\GAL4GMR.PF fails to suppress the 'small-eye' phenotype found in rprScer\UAS.cZa; Scer\GAL4GMR.PF flies.
Loss of one genomic copy of ninaA, using the amorphic allele ninaAE110V, protects the outer photoreceptor cells from rprScer\UAS.cZa-induced apoptosis. This anti-apoptotic effect is dose-dependent, as complete loss of ninaA leads to further protection.
Scer\GAL4srp.D.cCa-mediated expression of rprScer\UAS.cZa and WScer\UAS.cZa leads to a high lethality rate during early larval development - these larvae are devoid of hemocytes and rarely reach the third instar larval stage.
Expression of both rprScer\UAS.cZa and WScer\UAS.cZa in the cortex and neuropile glia from late embryonic stage onwards, under the control of Scer\GAL4nrv2.PS at 18[o]C and switching to a high temperature (25[o]C) shortly after hatching allows most animals to develop to late larval stages. In these larvae, glial cell death sets in around hatching and is more or less complete by the early 2nd instar. These larval brains exhibit a greatly increased number of intra-neuropilar tracheal branches. Six or more secondary branches split off the perineuropilar plexus at variable positions and penetrate the neuropile. Larvae lacking cortex and neuropile glia (but retaining surface glia) show severe behavioural deficits, characterised by reduced peristaltic movement and feeding, and a constant 'tremor' of the pharyngeal and body wall musculature.
Males co-expressing rprScer\UAS.cZa and WScer\UAS.cZa under the control of Scer\GAL4IFa.PT show no difference in male-female courtship behaviour compared to control males when naive males are allowed to court virgin wild-type females. However, the mutant males show significant male-male courtship behaviour when naive males are allowed to court males.
Animals co-expressing rprScer\UAS.cZa and WScer\UAS.cZa under the control of Scer\GAL4P2.4.Pdf are arrhythmic for locomotor activity under constant light conditions at a constant temperature of 25[o]C, but become rhythmic almost immediately after being exposed to a temperature cycle of 12 hours at 25[o]C and 12 hours at 30[o]C. There is a peak at the beginning of the cryophase and a smaller peak at the early thermophase. The flies show a dispersed anticipatory activity starting around the middle of the thermophase, although transient cycles are not clear.
Co-expression of BacA\p35Scer\UAS.cHa partially restores hemolymph trehalose levels in larvae expressing rprScer\UAS.cZa under the control of Scer\GAL4Akh.PL. Co-expression of BacA\p35Scer\UAS.cHa partially reduces the resistance to starvation-induced death seen in flies expressing rprScer\UAS.cZa under the control of Scer\GAL4Akh.PL.
The electroantennogram (EAG) rhythm in response to ethyl acetate (measured on the second day of constant darkness conditions) in flies co-expressing rprScer\UAS.cZa and WScer\UAS.cZa under the control of Scer\GAL4P2.4.Pdf is similar to that of wild-type flies.
Third instar larvae co-expressing both rprScer\UAS.cZa and WScer\UAS.cZa under the control of Scer\GAL41118 have no obvious defects in the Bolwig's nerve axonal projections although the lateral neurons are completely missing.
Co-expression of BacA\p35Scer\UAS.cHa completely blocks cell death caused by expression of rprScer\UAS.cZa under the control of Scer\GAL4en-e16E. Animals expressing both BacA\p35Scer\UAS.cHa and rprScer\UAS.cZa under the control of Scer\GAL4en-e16E survive to adulthood with largely normal wings.
Co-expression of WScer\UAS.cZa and rprScer\UAS.cZa (when driven by Scer\GAL4Gp150-52A) results in ectopic midline cell death. The addition of thScer\UAS.T:Ivir\HA1 suppresses this phenotype, sometimes to wild-type.
When grimScer\UAS.cNa and rprScer\UAS.cZa are coexpressed, all the midline glia and some VUM neurons are eliminated. When grimScer\UAS.cNa and WScer\UAS.cZa are coexpressed, all the midline glia and VUM neurons are eliminated.
Mediated expression of both rprScer\UAS.cZa and WScer\UAS.cZa causes a striking loss of glial cells, significant defects in axon guidance scaffold are detected. VUM neurons exhibit greatly reduced sensitivity: cells are normal in position, number and morphology. Two copies of each construct completely eliminates all midline glia and dramatic loss of VUM neurons. Results indicate all midline glia are capable of undergoing cell death and suggests the midline neurons and glia may have different sensitivities to rpr and W expression. Simultaneous expression of BacA\p35Scer\UAS.cHa causes no ectopic cell death demonstrating that midline cell death induced by rpr and W require the functions or one or more caspases.
Expression of BacA\p35Scer\UAS.P\T.cBa rescues the eye phenotypes seen when rprScer\UAS.cZa is expressed under the control of Scer\GAL4GMR.PF at 24[o]C. In females, eye size is increased compared to wild type (but is still small compared to the expression of BacA\p35Scer\UAS.P\T.cBa alone), whereas in males the eye size seen is comparable to that seen in wild type. Expression of BacA\p35Scer\UAS.P\T.cBa eliminates eye roughness in both males and females.
Expression of Zzzz\E4orf4Scer\UAS.cPa partially suppresses the semi-lethality seen when rprScer\UAS.cZa is expressed under the control of Scer\GAL4GMR.PF at 18[o]C. 27.4% of pupae eclose (compared to 0.5%). The reduction in eye size is also partially suppressed.
Co-expression of rprScer\UAS.cZa with BacA\p35Scer\UAS.cHa (both under the control of Scer\GAL4unspecified) in wgl-17 wing disc clones results in most or all of the cells in the clone being `undead'. None of these clones are associated with large, neoplastic outgrowths, and they grow slowly, forming abnormally small clones.