FlyBase Allele Report: Dmel\rpr[UAS.cZa]

Expression of rpr^Scer\UAS.cZa under the control of Scer\GAL4^{Myo31DF-NP0001} (combined with tub-Gal80[ts] for added temporal control) induces apoptosis but also leads to increased number of proliferating (EdU+) cells in the posterior midgut epithelium.

(Obata et al., 2018)

Expression of rpr^Scer\UAS.cZa under the control of Scer\GAL4^ptc-559.1 is lethal, but are viable when Scer\GAL80^αTub84B.PL is expressed in early development. Robust cell death is seen in the wing disc 12 hours after Scer\GAL4 de-repression.

When larvae expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^ptc-559.1 are irradiated, less cell death is seen in the wing disc in comparison with irradiated controls. The cells in the A compartment are the first to benefit, but the protective effect is able to reach the P compartment.

(Bilak et al., 2014)

Expression of rpr^Scer\UAS.cZa under the control of Scer\GAL4^GMR.PF results in almost complete loss of ommatidia in the eye at 29[o]C. Only 20% of flies are able to eclose. Less severe phenotypes are seen at 24[o]C, with 56% of flies able to eclose. At 18[o]C only 0.5% of flies eclose.

(Pechkovsky et al., 2013)

Expression of rpr^Scer\UAS.cZa under the control of Scer\GAL4^Gp150-52A induces a low level of cell death in embryonic CNS midline cells.

(Zhou et al., 2013)

Expression of rpr^Scer\UAS.cZa under the control of Scer\GAL4^btl.PU causes frequent gaps in the dorsal trunk. No loss of the salivary ducts is seen.

(Kerman and Andrew, 2010)

Expression of rpr^Scer\UAS.cZa in the developing eye under the control of Scer\GAL4^GMR.PF induces a small eye phenotype.

Down-regulation of endogenous th protein through ectopic expression of rpr^Scer\UAS.cZa in all four cells of the adult sensory organ, under the control of Scer\GAL4^sca-109-68 results in the loss of bristles on the adult notum, indicating a defect in only the shaft cell. In SOP lineages that express rpr^Scer\UAS.cZa, the SOP divdes four times, as in wild-type, makes the same five cell types and shows the same developmental time-course, beginning 16 hours after puparium formation.

(Koto et al., 2009)

Ectopic expression of rpr^Scer\UAS.cZa, under the control of Scer\GAL4^ninaE.PT induces a progressive loss of outer photoreceptor cells that can be visualised in tangential plastic retina sections of 2-day old flies.

(Mendes et al., 2009)

Expression of rpr^Scer\UAS.cZa using Scer\GAL4^Gr66a.PD abolishes the L-canavanine-induced proboscis retraction seen in wild type flies.

(Mitri et al., 2009)

The eclosion of flies expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^Eh.2.4 under photoperiod conditions lacks the prominent lights-on response seen in wild-type flies. Unlike in controls, advancing the lights on signal by one or two hours does not swell the leading edge of the eclosion distribution.

(McNabb and Truman, 2008)

Stage 16 embryos expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^gcm-rA87.C show a strong reduction in the number of glial cells. The brain neuropile is distorted and is not compartmentalised in the mutant embryos (in wild type the neuropile is subdivided by glial septa into distinct compartments). The glial sheath that is normally formed by surface glia around the brain is missing in the mutant embryos.

Late stage embryos expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^gcm-rA87.C show abnormalities in movement; peristaltic waves are absent and the embryos show incessant movement which consists of uncoordinated contractions of individual segments. The amplitude of the contractions is reduced compared to controls.

Animals expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^nrv2.PS and raised at 18[o]C mostly develop to late larval stages. In these larvae, most glial cells are still present in the first instar, but glial cell death occurs during the second instar. Late larvae show severe behavioural defects, which are characterised by strongly reduced peristaltic movement and feeding and a constant "tremor" of the pharyngeal and body wall musculature.

(Pereanu et al., 2007)

Males expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^IFa.PT show no difference in male-female courtship behaviour compared to control males when naive males are allowed to court virgin wild-type females. However, the mutant males show significant male-male courtship behaviour when naive males are allowed to court males.

(Terhzaz et al., 2007)

Flies that express rpr^Scer\UAS.cZa under the control of Scer\GAL4^Fmrf.PS show ablation of Tv neurons. These flies complete pupal ecdysis but show weaker pre-ecdysis contractions than controls.

Flies that express rpr^Scer\UAS.cZa under the control of Scer\GAL4^Eh.2.4 show ablation of VM neurons. These flies complete pupal ecdysis without severe defects or lethality but ecdysis is delayed by around 4 minutes, resulting in a longer period of pre-ecdysis.

Expression of rpr^Scer\UAS.cZa under the control of Scer\GAL4^Ccap.PP results in ablation of the 27/704 neurons. These flies fail to initiate ecdysis contractions and cannot complete head eversion. Instead, they show prolonged pre-ecdysis contractions for about 25 minutes, followed by weak random contractions of the abdomen for the next 50 minutes.

(Kim et al., 2006)

Males in which most npf-expressing cells have been ablated (by expression of rpr^Scer\UAS.cZa under the control of Scer\GAL4^nf.1) display subnormal courtship activity toward virgin females, partly because of prolonged courtship initiation latency before commencement of active courtship.

Males in which most npf-expressing cells have been ablated (by expression of rpr^Scer\UAS.cZa under the control of Scer\GAL4^nf.1) show subtle but consistently subnormal 'evening anticipation' (ie, the gradual increase in locomotor activity before the light-to-dark transition): after the quiescent midday period, activity levels are elevated slightly earlier compared to controls.

(Lee et al., 2006)

Expression of rpr^Scer\UAS.cZa in pacemaker neurons under the control of Scer\GAL4^P2.4.Pdf results in varying degrees of ablation of the normal compliment of eight PDF neurons per larval brain. However, this does not affect the larval response to light.

Ablation of PDF neurons through expression of rpr^Scer\UAS.cZa under the control of Scer\GAL4^tim.Scer\UAS does not affect the larval response to light.

(Hassan et al., 2005)

Severe lethality is seen when rpr^Scer\UAS.cZa is expressed using Scer\GAL4^GMR.PU, presumably due to leaky Scer\GAL4 expression. Animals also show a small-eye phenotype.

Expression of rpr^Scer\UAS.cZa using Scer\GAL4^Rh3.PP does not cause any lethality. However, R7 photoreceptor neurons are absent in some ommatidia, and this phenotype worsens significantly with age.

(Kanuka et al., 2005)

Expression of rpr^Scer\UAS.cZa, under the control of Scer\GAL4^P2.4.Pdf, causes ablation of the LNv. This has no apparent effect on 5-HT arborization. Further, ablation of the LNv and Rh5 photoreceptors, by expression of rpr^Scer\UAS.cZa driven by both Scer\GAL4^P2.4.Pdf and Scer\GAL4^Rh5.PT, does not affect 5-HT arborization.

(Rodriguez Moncalvo and Campos, 2005)

Larvae that express rpr^Scer\UAS.cZa under the control of Scer\GAL4^Eh.2.4 show ablation of the EH neurons. Only 31% of these larvae survive from first to third larval instar. The majority of dead larvae have fluid-filled trachea. Most Scer\GAL4^Eh.2.4>rpr^Scer\UAS.cZa larvae show similar ecdysis timings to wild-type larvae. However, Scer\GAL4^Eh.2.4>rpr^Scer\UAS.cZa larvae can occasionally show unusually light mouthpart pigmentation and differences in the amount of pigmentation between the two sides of the mouth plates.

Larvae that express rpr^Scer\UAS.cZa under the control of both Scer\GAL4^Eh.2.4 and Scer\GAL4^Ccap.PP show ablation of both the EH and CCAP neurons. These larvae also show a "buttoned-up" phenotype in which the mouthparts of the second instar are not shed and are present anterior to those of the third instar. The onset of pre-ecdysis is significantly delayed in these larvae compared to controls.

(Clark et al., 2004)

Expression of rpr^Scer\UAS.cZa under the control of Scer\GAL4^slbo.2.6 results in apoptotic cells in stage 10 egg chambers, in contrast to wild-type egg chambers; TUNEL labeling is seen in the border cells, centripetal cells and posterior follicle cells.

(Geisbrecht and Montell, 2004)

Expression of rpr^Scer\UAS.cZa under the control of Scer\GAL4^Akh.PL does not produce any noticeable defects in growth, metamorphosis, eclosion or longevity. Adults show normal reproductive capabilities and courtship behaviour. Hemolymph trehalose levels are reduced to 7-26% of normal levels in larvae expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^Akh.PL, while glucose levels are unaffected. The triglyceride content of the fat body is normal. Flies expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^Akh.PL show resistance to starvation-induced death. Flies expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^Akh.PL show similar locomotor activity rhythms to wild-type flies when fed on 4% sucrose-agar medium. Under starvation conditions, most flies expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^Akh.PL do not show pronounced starvation-induced hyperactivity (which is seen in wild-type flies).

(Lee and Park, 2004)

Expression of rpr^Scer\UAS.cZa under the control of Scer\GAL4^Ccap.PP results in the ablation of Ccap-expressing neurons in the larval

central nervous system.

Expression of rpr^Scer\UAS.cZa under the control of Scer\GAL4^Ccap.PP

is not lethal during the larval stages. These animals are able to

shed their old cuticle at the end of the moult to the 3rd instar.

The initiation of ecdysis behaviour from the 2nd to 2rd larval instar

occurs and the larvae use this behaviour to free themselves from the

2nd instar cuticle. The duration of events up to the onset of pre-ecdysis

is normal, but there is a modest but significant lengthening of pre-ecdysis

behaviour (although the pre-ecdysis behaviour itself is normal). The

biting period, which occurs between the end of pre-ecdysis and ecdysis

onset, and the duration of ecdysis itself, are significantly extended

compared to wild type. The larvae show anterior to posterior peristaltic

waves interspersed with the typically occurring posterior to anterior

waves, which is never seen in wild-type animals. The waves moving

in the anterior to posterior direction do not aid in breaking the old

cuticle, so the time to successful shedding of the old cuticle is lengthened.

Most animals expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^Ccap.PP

die during the pupal stage. Early pupal stage animals show defects

such as incomplete head eversion, retracted posterior cuticle due to

failure to transition from pre-ecdysis to ecdysis and incomplete shedding

of tracheal lining. Pharate adults have defects in adult head formation

and in leg and wing extension. Animals expressing rpr^Scer\UAS.cZa

under the control of Scer\GAL4^Ccap.PP initiate normal pre-ecdysis

behaviour before pupal ecdysis, however, this behaviour lasts significantly

longer than in controls and is not followed by head eversion. Instead,

abdominal pre-ecdysis movements eventually cease and are followed by

a period that resembles the postecdysis period seen in controls.

About 10% of animals expressing rpr^Scer\UAS.cZa under the control

of Scer\GAL4^Ccap.PP form relatively normal heads at the end of

metamorphosis, and these animals are usually able to exit from the

pupal case. The developmental and behavioural events that normally

occur at eclosion do occur in these animals, although there are differences

in the duration or number of events compared to wild type. For example,

tracheal filling takes longer than normal, while the ptilinum is deployed

normally. The bouts of rapid anteriorly directed peristalses of ecdysis

proper occur in these animals, but they are relatively ineffective

at propelling the animal forward, because the abdomen is not distended

by this time. Although most of these animals eventually succeed in

eclosing, it takes much longer than normal.

The eclosion profile of animals expressing rpr^Scer\UAS.cZa under

the control of Scer\GAL4^Ccap.PP is different from that of control

animals; the temporal gate of eclosion is lengthened, with significant

emergence occurring in the late night/predawn period. There is also

a significant diminution in the amplitude of the eclosion burst that

occurs immediately following lights-on. There is no consistent difference

in the peak time of eclosion between animals expressing rpr^Scer\UAS.cZa

under the control of Scer\GAL4^Ccap.PP and control animals in light-dark

or constant darkness conditions.

(Park et al., 2003)

Most flies expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^GMR.PF die shortly after pupariation. Flies expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^rdgC.PK survive well to adulthood and show no defects in walking behaviour.

(Keller et al., 2002)

Expression of rpr^Scer\UAS.cZa under the control of Scer\GAL4^Gp150-52A in embryos in the central nervous system midline does not induce ectopic cell death.

(Wing et al., 2002)

Flies expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^Eh.2.4 show a similar rise in heart rate during the last 10 hours of adult development (before eclosion) as seen in wild-type flies, differing significantly only during the smooth/grainy stage and grainy stage. Flies expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^Eh.2.4 fail to fill their tracheoles completely with air at any stage during the last 2.9 hours of development before eclosion, in contrast to wild type which have air filled tracheoles by 0.9 hours before eclosion. In contrast to wild-type flies, injection of M.sexta ecdysis-triggering hormone (ETH) is ineffective in inducing premature tracheal filling around the ventral CNS or in the tracheal sacs overlying the brain in flies expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^Eh.2.4. Injection of M.sexta ETH into these flies at the grainy stage also has no effect on the heart rate, in contrast to wild-type flies. Very few flies expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^Eh.2.4 attempt to ecdyse after neck ligation and there is no stage dependent pattern to these ecdyses (this response differs from wild-type flies).

(Baker et al., 1999)

The locomotor activity of flies expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^P2.4.Pdf is not entirely normal. The flies are entrained during 12 hour light:12 hour dark (LD) cycles, but the evening locomotor phase is advanced by at least 0.5 hours compared to wild-type flies. 63% of flies are rhythmic for the entire duration of a 9 day period under constant darkness. The proportion of rhythmic individuals decreases over time under constant darkness. Flies that are persistently rhythmic tend to have short periods.

(Renn et al., 1999)

Expression of rpr^Scer\UAS.cZa under the control of Scer\GAL4^Eh.2.4 results in semilethality, with most animals dying during the larval period, crawling out of the food before dying. Most of the larvae have tracheal abnormalities; 80% have dorsal tracheae filled with fluid. Old tracheae are seen inside new ones. Eh expressing neurons die during the first or second larval stages in animals expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^Eh.2.4. The neurons appear to disintegrate progressively. 30%-34% of animals expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^Eh.2.4 eclose as fertile, viable adults. Defects are seen during pre-eclosion, when the timing of inflation of the trachea relative to ptilinum extension is altered compared to wild-type (tracheal inflation is delayed in approximately a third of cases). There is also a dramatic increase in the latency from ptilinum extension to adult emergence. The organisation of eclosion behaviour into bouts that is seen in wild-type flies is absent in flies expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^Eh.2.4. These flies display the four characteristic eclosion behaviours (head expansion, head thrusts, thoracic contractions and abdominal contractions), but once these behaviours begin they are performed almost continuously without periods of quiescence. The behaviours also seem weaker than in wild-type flies. The flies fail to undergo normal postemergence expansion and hardening of the adult cuticle after they have eclosed, and more than two-thirds of flies fail to undergo wing expansion. The eclosion of the flies under photoperiod conditions lacks the prominent lights-on response seen in wild-type flies, although the flies show a robust free-running eclosion rhythm under constant darkness.

(McNabb et al., 1997)

Scer\GAL4^Gp150-52A-mediated expression of rpr^Scer\UAS.cZa alone is not sufficient to induce substantial apoptosis in the embryonic CNS midline cells. The midline cells survive and are capable of carrying out normal axon guidance functions (axon scaffold organisation is normal).

(Zhou et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^Gp150-52A, rpr^UAS.cZa has abnormal cell death | embryonic stage phenotype, enhanceable by morgue^UAS.cZa/Scer\GAL4^Gp150-52A

(Zhou et al., 2013)

Scer\GAL4^Gp150-52A, rpr^UAS.cZa has abnormal cell death | embryonic stage phenotype, enhanceable by morgue^G421S.UAS/Scer\GAL4^Gp150-52A

(Zhou et al., 2013)

Scer\GAL4^Gp150-52A, rpr^UAS.cZa has abnormal cell death | embryonic stage phenotype, enhanceable by morgue^G421C.UAS/Scer\GAL4^Gp150-52A

(Zhou et al., 2013)

Scer\GAL4^Gp150-52A, rpr^UAS.cZa has abnormal cell death | embryonic stage phenotype, enhanceable by morgue^G421A.UAS/Scer\GAL4^Gp150-52A

(Zhou et al., 2013)

Suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, Scer\GAL80^αTub84B.PL, hid^UAS.cZa, rpr^UAS.cZa has radiation resistant | cell non-autonomous phenotype, suppressible | partially by +/Df(3L)mir-ban^Δ1

(Bilak et al., 2014)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has visible | adult stage phenotype, suppressible by Diap1^{UAS.Tag:MYC.cBa}, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has partially lethal - majority die phenotype, suppressible by Diap1^{UAS.Tag:MYC.cBa}, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has visible | adult stage phenotype, suppressible | male limited by BacA\p35^UASp.cBa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has abnormal size | adult stage phenotype, suppressible | male limited by BacA\p35^UASp.cBa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has partially lethal - majority die phenotype, suppressible | partially by HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has some die during pupal stage phenotype, suppressible | partially by HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has visible | adult stage phenotype, suppressible | partially by HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has abnormal size | adult stage phenotype, suppressible | partially by HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

rpr^UAS.cZa has increased cell death phenotype, suppressible by ninaA[+]/ninaA^E110V/Scer\GAL4^ninaE.PT

(Mendes et al., 2009)

Scer\GAL4^SIFa.PT, rpr^UAS.cZa has abnormal courtship behavior | male phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^SIFa.PT

(Terhzaz et al., 2007)

BacA\p35^UAS.cHa, Scer\GAL4^αTub84B.PZ, rpr^UAS.cZa has hyperplasia | somatic clone phenotype, suppressible by puc^UAS.cMa

(Ryoo et al., 2004)

Scer\GAL4^en-e16E, rpr^UAS.cZa has lethal | embryonic stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en-e16E

(Ryoo et al., 2002)

Scer\GAL4^en-e16E, rpr^UAS.cZa has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en-e16E

(Ryoo et al., 2002)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has lethal phenotype, suppressible by Diap1^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Wing et al., 2001)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has visible phenotype, suppressible by Diap1^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Wing et al., 2001)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has increased cell death phenotype, suppressible by Diap1^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Wing et al., 2001)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has increased cell death phenotype, suppressible | partially by Diap1^SL

(Wing et al., 2001)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has lethal phenotype, suppressible by Diap1^SL

(Wing et al., 2001)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has visible phenotype, suppressible | partially by Diap1^SL

(Wing et al., 2001)

Scer\GAL4^P2.4.Pdf, rpr^UAS.cZa has abnormal locomotor rhythm phenotype, suppressible | partially by BacA\p35^UAS.cHa, Scer\GAL4^P2.4.Pdf

(Renn et al., 1999)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has lethal phenotype, suppressible by Diap2^UAS.cWa, Scer\GAL4^GMR.PF

(Wing et al., 1998)

Suppressor of

Statement

Reference

rpr^UAS.cZa, Scer\GAL4^GMR.PF is a suppressor | partially of visible | female limited | adult stage phenotype of BacA\p35^UASp.cBa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

rpr^UAS.cZa, Scer\GAL4^GMR.PF is a suppressor | partially of abnormal size | female limited | adult stage phenotype of BacA\p35^UASp.cBa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Scer\GAL4^vg.PM/rpr^UAS.cZa is a suppressor | partially of lethal | pupal stage phenotype of Scer\GAL4^vg.PM, stwl^UY823

(Brun et al., 2006)

Other

Statement

Reference

Scer\GAL4^GMR45F08, hid^UAS.cZa, rpr^UAS.cZa has abnormal neuroanatomy | larval stage phenotype

(Andrade et al., 2019)

Scer\GAL4^GMR45F08, hid^UAS.cZa, rpr^UAS.cZa has lethal - all die during embryonic stage phenotype

(Andrade et al., 2019)

Scer\GAL4^GMR45F08, hid^UAS.cZa, rpr^UAS.cZa has lethal - all die before end of larval stage phenotype

(Andrade et al., 2019)

Scer\GAL4^ptc-559.1, Scer\GAL80^αTub84B.PL, hid^UAS.cZa, rpr^UAS.cZa has lethal - all die before end of larval stage phenotype

(Bilak et al., 2014)

Scer\GAL4^ptc-559.1, Scer\GAL80^αTub84B.PL, hid^UAS.cZa, rpr^UAS.cZa has radiation resistant | cell non-autonomous phenotype

(Bilak et al., 2014)

Scer\GAL4^ptc-559.1, Scer\GAL80^αTub84B.PL, hid^UAS.cZa, rpr^UAS.cZa has radiation resistant | somatic clone | cell non-autonomous phenotype

(Bilak et al., 2014)

Scer\GAL4^ptc-559.1, Scer\GAL80^αTub84B.PL, hid^UAS.cZa, rpr^UAS.cZa has increased cell death | somatic clone phenotype

(Bilak et al., 2014)

Scer\GAL4^srp.D.cCa, hid^UAS.cZa, rpr^UAS.cZa has lethal - all die during larval stage phenotype

(Shia et al., 2009)

Scer\GAL4^nrv2.PS, hid^UAS.cZa, rpr^UAS.cZa has lethal | temperature conditional phenotype

(Pereanu et al., 2007)

Scer\GAL4^nrv2.PS, hid^UAS.cZa, rpr^UAS.cZa has increased cell death | temperature conditional phenotype

(Pereanu et al., 2007)

Scer\GAL4^nrv2.PS, hid^UAS.cZa, rpr^UAS.cZa has abnormal neuroanatomy | temperature conditional phenotype

(Pereanu et al., 2007)

Scer\GAL4^P2.4.Pdf, hid^UAS.cZa, rpr^UAS.cZa has abnormal behavior phenotype

(Yoshii et al., 2005)

BacA\p35^UAS.cHa, Scer\GAL4^αTub84B.PZ, rpr^UAS.cZa has hyperplasia | somatic clone phenotype

(Ryoo et al., 2004)

Scer\GAL4¹¹¹⁸, hid^UAS.cZa, rpr^UAS.cZa has abnormal neuroanatomy phenotype

(Malpel et al., 2002)

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, rpr^UAS.cZa has viable phenotype

(Ryoo et al., 2002)

Scer\GAL4^Gp150-52A, morgue^EP2367, rpr^UAS.cZa has increased cell death phenotype

(Wing et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Gp150-52A, rpr^UAS.cZa has ventral midline of embryo | embryonic stage phenotype, enhanceable by morgue^UAS.cZa/Scer\GAL4^Gp150-52A

(Zhou et al., 2013)

Scer\GAL4^Gp150-52A, rpr^UAS.cZa has ventral midline of embryo | embryonic stage phenotype, enhanceable by morgue^G421S.UAS/Scer\GAL4^Gp150-52A

(Zhou et al., 2013)

Scer\GAL4^Gp150-52A, rpr^UAS.cZa has ventral midline of embryo | embryonic stage phenotype, enhanceable by morgue^G421C.UAS/Scer\GAL4^Gp150-52A

(Zhou et al., 2013)

Scer\GAL4^Gp150-52A, rpr^UAS.cZa has ventral midline of embryo | embryonic stage phenotype, enhanceable by morgue^G421A.UAS/Scer\GAL4^Gp150-52A

(Zhou et al., 2013)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has midline glial cell phenotype, enhanceable by grim^UAS.cNa/Scer\GAL4^Gp150-52A

(Wing et al., 1998)

Suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, Scer\GAL80^αTub84B.PL, hid^UAS.cZa, rpr^UAS.cZa has wing disc | cell non-autonomous phenotype, suppressible | partially by +/Df(3L)mir-ban^Δ1

(Bilak et al., 2014)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has eye phenotype, suppressible by Diap1^{UAS.Tag:MYC.cBa}, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has ommatidium phenotype, suppressible by Diap1^{UAS.Tag:MYC.cBa}, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has eye phenotype, suppressible by BacA\p35^UASp.cBa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has ommatidium phenotype, suppressible by BacA\p35^UASp.cBa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has eye phenotype, suppressible | partially by HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has eye phenotype, suppressible by Diap1^UAS.Tag:FLAG, Scer\GAL4^GMR.PF

(Koto et al., 2009)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has eye phenotype, suppressible by Diap1^UAS.Venus, Scer\GAL4^GMR.PF

(Koto et al., 2009)

rpr^UAS.cZa has outer photoreceptor cell phenotype, suppressible by ninaA[+]/ninaA^E110V/Scer\GAL4^ninaE.PT

(Mendes et al., 2009)

Scer\GAL4^Rh3.PP, rpr^UAS.cZa has rhabdomere R7 phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^Rh3.PP

(Kanuka et al., 2005)

BacA\p35^UAS.cHa, Scer\GAL4^αTub84B.PZ, rpr^UAS.cZa has wing disc | somatic clone phenotype, suppressible by puc^UAS.cMa

(Ryoo et al., 2004)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has eye phenotype, suppressible by Diap1^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Wing et al., 2001)

Scer\GAL4^Gp150-52A, hid^UAS.cZa, rpr^UAS.cZa has ventral midline of embryo phenotype, suppressible | partially by Diap1^UAS.Tag:HA, Scer\GAL4^Gp150-52A

(Wing et al., 2001)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has eye phenotype, suppressible | partially by Diap1^SL

(Wing et al., 2001)

Scer\GAL4^Gp150-52A, hid^UAS.cZa, rpr^UAS.cZa has ventral midline of embryo phenotype, suppressible | partially by Diap1^UAS.Tag:HA

(Wing et al., 2001)

Scer\GAL4^GMR.PF, rpr^UAS.cZa has midline glial cell phenotype, suppressible by Scer\GAL4^Gp150-52A/Diap2^UAS.cWa

(Wing et al., 1998)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, rpr^UAS.cZa has eye phenotype, non-suppressible by Diap1^{PRAP.UAS.Venus}, Scer\GAL4^GMR.PF

(Koto et al., 2009)

Enhancer of

Statement

Reference

Scer\GAL4^Gp150-52A/rpr^UAS.cZa is an enhancer of midline glial cell phenotype of Scer\GAL4^Gp150-52A, grim^UAS.cNa

(Wing et al., 1998)

Suppressor of

Statement

Reference

rpr^UAS.cZa, Scer\GAL4^GMR.PF is a suppressor | partially of eye | female limited phenotype of BacA\p35^UASp.cBa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Other

Statement

Reference

Scer\GAL4^GMR45F08, hid^UAS.cZa, rpr^UAS.cZa has larval posterior inferior protocerebrum phenotype

(Andrade et al., 2019)

Scer\GAL4^GMR45F08, hid^UAS.cZa, rpr^UAS.cZa has embryonic/larval fan-shaped body - primordial phenotype

(Andrade et al., 2019)

Scer\GAL4^ptc-559.1, Scer\GAL80^αTub84B.PL, hid^UAS.cZa, rpr^UAS.cZa has wing disc | cell non-autonomous phenotype

(Bilak et al., 2014)

Scer\GAL4^ple.PF, grim^UAS.cOCa, hid^UAS.cZa, rpr^UAS.cZa has neurite & dopaminergic neuron phenotype

(Palgi et al., 2009)

Scer\GAL4^Hml.PG, hid^UAS.cZa, rpr^UAS.cZa has embryonic/larval hemocyte phenotype

(Shia et al., 2009)

Scer\GAL4^Pxn.PS, hid^UAS.cZa, rpr^UAS.cZa has embryonic/larval hemocyte phenotype

(Shia et al., 2009)

Scer\GAL4^srp.D.cCa, hid^UAS.cZa, rpr^UAS.cZa has embryonic/larval hemocyte phenotype

(Shia et al., 2009)

Scer\GAL4^nrv2.PS, hid^UAS.cZa, rpr^UAS.cZa has embryonic/larval ganglionic tracheal branch | temperature conditional phenotype

(Pereanu et al., 2007)

Scer\GAL4^nrv2.PS, hid^UAS.cZa, rpr^UAS.cZa has glial cell | temperature conditional phenotype

(Pereanu et al., 2007)

BacA\p35^UAS.cHa, Scer\GAL4^αTub84B.PZ, rpr^UAS.cZa has wing disc | somatic clone phenotype

(Ryoo et al., 2004)

Scer\GAL4¹¹¹⁸, hid^UAS.cZa, rpr^UAS.cZa has larval LN period neuron phenotype

(Malpel et al., 2002)

Scer\GAL4^Gp150-52A, morgue^EP2367, rpr^UAS.cZa has presumptive embryonic/larval central nervous system phenotype

(Wing et al., 2002)

Scer\GAL4^Gp150-52A, morgue^EP2367, rpr^UAS.cZa has midline glial cell phenotype

(Wing et al., 2002)

Scer\GAL4^Gp150-52A, grim::rpr^rg.UAS, rpr^UAS.cZa has ventral midline of embryo phenotype

(Wing et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of W^Scer\UAS.cZa and rpr^Scer\UAS.cZa under the control of Scer\GAL4^ptc-559.1 is lethal, but is viable when Scer\GAL80^αTub84B.PL is expressed until the third instar larval stage. Robust cell death is seen in the wing disc 12 hours after Scer\GAL4 de-repression.

When larvae expressing W^Scer\UAS.cZa and rpr^Scer\UAS.cZa under the control of Scer\GAL4^ptc-559.1 are irradiated (and expression is repressed until 12 hours prior to irradiation using Scer\GAL80^αTub84B.PL), less cell death is seen in the wing disc in comparison with irradiated controls. A heterozygous Df(3L)ban^Δ1 background has no effect on the amount of cell death, but when de-repression occurs 6 hours prior to irradiation (which results in a less dramatic reduction in cell death compared to 12 hours), heterozygous Df(3L)ban^Δ1 partially suppresses the level of protection seen when W^Scer\UAS.cZa and rpr^Scer\UAS.cZa are expressed alone.

When wing discs containing W^Scer\UAS.cZa and rpr^Scer\UAS.cZa expressing clones (repressed during early development using Scer\GAL80^αTub84B.PL)) are irradiated, less cell death is seen outside of the clones in comparison with irradiated controls.

(Bilak et al., 2014)

Expression of th^{Scer\UAS.T:Hsap\MYC.cBa} almost completely restores the eye phenotypes seen when rpr^Scer\UAS.cZa is expressed under the control of Scer\GAL4^GMR.PF at 29[o]C. Eye size is 98% of control and no roughness or other morphological abnormalities are observed. The loss of viability seen in flies expressing rpr^Scer\UAS.cZa is rescued upon co-expression of th^{Scer\UAS.T:Hsap\MYC.cBa}.

(Pechkovsky et al., 2013)

Expression of morgue^Scer\UAS.cZa enhances the embryonic CNS midline cell death phenotype seen when rpr^Scer\UAS.cZa is expressed under the control of Scer\GAL4^Gp150-52A.

Expression of morgue^{G421A.Scer\UAS} enhances the embryonic CNS midline cell death phenotype seen when rpr^Scer\UAS.cZa is expressed under the control of Scer\GAL4^Gp150-52A.

Expression of morgue^{G421C.Scer\UAS} enhances the embryonic CNS midline cell death phenotype seen when rpr^Scer\UAS.cZa is expressed under the control of Scer\GAL4^Gp150-52A.

Expression of morgue^{G421S.Scer\UAS} enhances the embryonic CNS midline cell death phenotype seen when rpr^Scer\UAS.cZa is expressed under the control of Scer\GAL4^Gp150-52A.

(Zhou et al., 2013)

Co-expression of W^Scer\UAS.cZa and rpr^Scer\UAS.cZa in the posterior signalling center under the control of Scer\GAL4^Antp-10 has no effect on lamellocyte differentiation.

(Sinenko et al., 2011)

Expression of th^{Scer\UAS.T:Zzzz\FLAG} or th^{Scer\UAS.T:Avic\GFP-YFP.Venus} in the developing eye under the control of Scer\GAL4^GMR.PF suppresses the rpr^Scer\UAS.cZa-induced 'small-eye' phenotype.

Expression of th^{N117K.H283Y.Scer\UAS.T:Avic\GFP-YFP.Venus} in the developing eye under the control of Scer\GAL4^GMR.PF fails to suppress the 'small-eye' phenotype found in rpr^Scer\UAS.cZa; Scer\GAL4^GMR.PF flies.

(Koto et al., 2009)

Loss of one genomic copy of ninaA, using the amorphic allele ninaA^E110V, protects the outer photoreceptor cells from rpr^Scer\UAS.cZa-induced apoptosis. This anti-apoptotic effect is dose-dependent, as complete loss of ninaA leads to further protection.

(Mendes et al., 2009)

Co-expression of W^Scer\UAS.cZa, grim^{Scer\UAS.cOCa} and rpr^Scer\UAS.cZa under the control of Scer\GAL4^ple.PF results in the volume of DA neurites being significantly diminished compared to controls.

(Palgi et al., 2009)

Scer\GAL4^Hml.PG-, Scer\GAL4^Pxn.PS- or Scer\GAL4^srp.D.cCa-mediated expression of rpr^Scer\UAS.cZa and W^Scer\UAS.cZa ablates larval hemocytes.

Scer\GAL4^srp.D.cCa-mediated expression of rpr^Scer\UAS.cZa and W^Scer\UAS.cZa leads to a high lethality rate during early larval development - these larvae are devoid of hemocytes and rarely reach the third instar larval stage.

(Shia et al., 2009)

Expression of both rpr^Scer\UAS.cZa and W^Scer\UAS.cZa in the cortex and neuropile glia from late embryonic stage onwards, under the control of Scer\GAL4^nrv2.PS at 18[o]C and switching to a high temperature (25[o]C) shortly after hatching allows most animals to develop to late larval stages. In these larvae, glial cell death sets in around hatching and is more or less complete by the early 2nd instar. These larval brains exhibit a greatly increased number of intra-neuropilar tracheal branches. Six or more secondary branches split off the perineuropilar plexus at variable positions and penetrate the neuropile. Larvae lacking cortex and neuropile glia (but retaining surface glia) show severe behavioural deficits, characterised by reduced peristaltic movement and feeding, and a constant 'tremor' of the pharyngeal and body wall musculature.

(Pereanu et al., 2007)

Males co-expressing rpr^Scer\UAS.cZa and W^Scer\UAS.cZa under the control of Scer\GAL4^IFa.PT show no difference in male-female courtship behaviour compared to control males when naive males are allowed to court virgin wild-type females. However, the mutant males show significant male-male courtship behaviour when naive males are allowed to court males.

Females co-expressing rpr^Scer\UAS.cZa and W^Scer\UAS.cZa under the control of Scer\GAL4^IFa.PT copulate more rapidly with male flies than control females.

(Terhzaz et al., 2007)

Animals co-expressing rpr^Scer\UAS.cZa and W^Scer\UAS.cZa under the control of Scer\GAL4^P2.4.Pdf are arrhythmic for locomotor activity under constant light conditions at a constant temperature of 25[o]C, but become rhythmic almost immediately after being exposed to a temperature cycle of 12 hours at 25[o]C and 12 hours at 30[o]C. There is a peak at the beginning of the cryophase and a smaller peak at the early thermophase. The flies show a dispersed anticipatory activity starting around the middle of the thermophase, although transient cycles are not clear.

(Yoshii et al., 2005)

Co-expression of BacA\p35^Scer\UAS.cHa partially restores hemolymph trehalose levels in larvae expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^Akh.PL. Co-expression of BacA\p35^Scer\UAS.cHa partially reduces the resistance to starvation-induced death seen in flies expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^Akh.PL.

(Lee and Park, 2004)

The electroantennogram (EAG) rhythm in response to ethyl acetate (measured on the second day of constant darkness conditions) in flies co-expressing rpr^Scer\UAS.cZa and W^Scer\UAS.cZa under the control of Scer\GAL4^P2.4.Pdf is similar to that of wild-type flies.

(Tanoue et al., 2004)

Third instar larvae co-expressing both rpr^Scer\UAS.cZa and W^Scer\UAS.cZa under the control of Scer\GAL4¹¹¹⁸ have no obvious defects in the Bolwig's nerve axonal projections although the lateral neurons are completely missing.

(Malpel et al., 2002)

Co-expression of BacA\p35^Scer\UAS.cHa completely blocks cell death caused by expression of rpr^Scer\UAS.cZa under the control of Scer\GAL4^en-e16E. Animals expressing both BacA\p35^Scer\UAS.cHa and rpr^Scer\UAS.cZa under the control of Scer\GAL4^en-e16E survive to adulthood with largely normal wings.

(Ryoo et al., 2002)

Co-expression of rpr^Scer\UAS.cZa and morgue^EP2367 under the control of Scer\GAL4^Gp150-52A in embryos in the central nervous system midline results in a dramatic loss of midline glia and neurons.

(Wing et al., 2002)

Co-expression of W^Scer\UAS.cZa and rpr^Scer\UAS.cZa (when driven by Scer\GAL4^Gp150-52A) results in ectopic midline cell death. The addition of th^{Scer\UAS.T:Ivir\HA1} suppresses this phenotype, sometimes to wild-type.

(Wing et al., 2001)

The locomotor activity defects of flies expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^P2.4.Pdf are partially suppressed by coexpression of BacA\p35^Scer\UAS.cHa.

(Renn et al., 1999)

When grim^Scer\UAS.cNa and rpr^Scer\UAS.cZa are coexpressed, all the midline glia and some VUM neurons are eliminated. When grim^Scer\UAS.cNa and W^Scer\UAS.cZa are coexpressed, all the midline glia and VUM neurons are eliminated.

(Wing et al., 1998)

Mediated expression of both rpr^Scer\UAS.cZa and W^Scer\UAS.cZa causes a striking loss of glial cells, significant defects in axon guidance scaffold are detected. VUM neurons exhibit greatly reduced sensitivity: cells are normal in position, number and morphology. Two copies of each construct completely eliminates all midline glia and dramatic loss of VUM neurons. Results indicate all midline glia are capable of undergoing cell death and suggests the midline neurons and glia may have different sensitivities to rpr and W expression. Simultaneous expression of BacA\p35^Scer\UAS.cHa causes no ectopic cell death demonstrating that midline cell death induced by rpr and W require the functions or one or more caspases.

(Zhou et al., 1997)

Xenogenetic Interactions

Statement

Reference

Expression of BacA\p35^{Scer\UAS.P\T.cBa} rescues the eye phenotypes seen when rpr^Scer\UAS.cZa is expressed under the control of Scer\GAL4^GMR.PF at 24[o]C. In females, eye size is increased compared to wild type (but is still small compared to the expression of BacA\p35^{Scer\UAS.P\T.cBa} alone), whereas in males the eye size seen is comparable to that seen in wild type. Expression of BacA\p35^{Scer\UAS.P\T.cBa} eliminates eye roughness in both males and females.

Expression of Zzzz\E4orf4^Scer\UAS.cPa partially suppresses the semi-lethality seen when rpr^Scer\UAS.cZa is expressed under the control of Scer\GAL4^GMR.PF at 18[o]C. 27.4% of pupae eclose (compared to 0.5%). The reduction in eye size is also partially suppressed.

(Pechkovsky et al., 2013)

Co-expression of BacA\p35^Scer\UAS.cHa suppresses the male-male courtship seen in males expressing rpr^Scer\UAS.cZa under the control of Scer\GAL4^IFa.PT.

(Terhzaz et al., 2007)

Coexpression of BacA\p35^Scer\UAS.cHa rescues the loss of R7 neurons caused by Scer\GAL4^Rh3.PP-mediated expression of rpr^Scer\UAS.cZa.

(Kanuka et al., 2005)

rpr^Scer\UAS.cZa; BacA\p35^Scer\UAS.cHa; Scer\GAL4^αTub84B.PZ somatic clones in the wing disc cause significant overgrowth of this disc. This overgrowth in suppressed by puc^Scer\UAS.cMa.

(Ryoo et al., 2004)

Complementation and Rescue Data

Comments

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Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

rpr^Scer\UAS.cZa

rpr^UAS.cMa

rpr^UAS.cZa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Zhou

(Zhou et al., 1997)

Secondary FlyBase IDs

FBal0082291

References (89)

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