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General Information
Symbol
Scer\GAL4SIFa.PT
Species
S. cerevisiae
Name
IFamide promoter construct of Terhaz
FlyBase ID
FBal0246774
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
SIFa-GAL4, SIFa2-Gal4
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A 1482bp fragment from immediately 5' of the SIFa coding region regulates expression of a GAL4 driver.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Associated Sequence Features
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Expression of Scer\GAL4SIFa.PT is found in a subset of four Scer\GAL4Kurs58 positive pars intercerebralis (PI) neurons. GFP reconstitution across synaptic partners (GRASP) shows a connection between Scer\GAL4SIFa.PT positive PI neurons and Scer\GAL4Clk.1.5 positive DN1p neurons.

Scer\GAL4SIFa.PT drives expression in 4 cells in the pars intercerebralis that extend processes throughout most of the brain neuropils, including most glmeruli of the antennal lobe. The cells labelled in the pars intercerebralis by Scer\GAL4SIFa.PT also express SIFa.

 
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Thermogenetic activation (using TrpA1UAS.(B).cKa at 29[o]C) of Scer\GAL4SIFa.PT cells in sated flies significantly increases preference for appetitive odors and proboscis extension reflex (PER) in response to sucrose, compared to controls; activation has no affect on PER of starved flies or avoidance of aversive odors in sated flies, compared to controls; activation significantly increases short-term and long-term food intake of sated flies, compared to controls, with no effect on locomotor activity; activation of a subset (restricted using Scer\GAL80tsh-GAL80) of Scer\GAL4SIFa.PT cells also significantly increases short-term food intake, compared to controls.

Inhibition (using Ork1Δ-C.UAS.EGFP) of Scer\GAL4SIFa.PT cells, in combination with a Gal80[ts] transgene to restrict expression to during the feeding experiment, does not affect food intake of starved flies, compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Scer\GAL4IFa.PT
Scer\GAL4SIFa.PT
Name Synonyms
IFamide promoter construct of Terhaz
Secondary FlyBase IDs
    References (14)